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ID BSBM preliminary; circular DNA; SYN; 3210 BP.
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AC ATCC67725;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector BSB- - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pcDpoly from pL1 & pcDV1 & BlueScribe M13+
RC pcDpolyB from pcDpoly & oligo
RC pXPRS+ or pCDpolyB+ from pcDpolyB & BlueScribe M13+
RC pXPRS- or pCDpolyB- from pcDpolyB & BlueScribe M13+
RC BSB+ from BlueScribe M13+ & oligo
RC BSB- from BlueScribe M13- & oligo
RA Pruitt S.C.;
RT "Expression vectors permitting cDNA cloning and enrichment for
RT specific sequences by hybridization/selection";
RL Gene 66:121-134(1988).
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CC Created by Moore, July 1995, under contract with NCBI.
CC Constructed from Bluescribe M13- by insertion of a synthetic BstXI
CC adapter between the PstI & XbaI sites of the polylinker region.
CC A plasmid vector containing convenient priming sites for sequence
CC analysis and synthesis of RNA in either direction from T3 and T7
CC promoters.
CC BSB+ (away from cloning site) and BSB- (toward cloning site) differ in
CC their orientation of the intergenic region from bacteriophage f1.
CC Contains the intergenic region from bacteriophage f1 permitting
CC synthesis of single-strand copies of the insert.
CC Restriction digests of the clone give the following sizes (kb):
CC NdeI/HindIII--2.45, 0.75; BamHI--3.2; NdeI--3.2; PvuII--2.65 +
CC smaller; PstI--uncut. (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NM (BSB-)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (ATCC)
CC HO (E.coli HB101)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (BlueScribe M13-)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. BlueScribe M13- remove XbaI-PstI 16bp
FT \ 909..925, MCS/3188bp
FT 2. oligo XbaI-BstXI-PstI 22bp ctagagccacccccctggtgca
FT -> BSB- 3210bp"
FT - 1..908
FT /note="BlueScribe M13- 1..908 908bp
FT XbaI = T^CTAGA
FT \ ctaga..."
FT - 909..930
FT /note="ctagagccacccccctggtgca 22bp
FT \ ...gtgca
FT PstI = CTGCA^G"
FT - 931..3210
FT /note="BlueScribe M13- 925..3204 2280bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-KpnI-SmaI-XbaI-BstXI-PstI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique BstXI-BamHI-KpnI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 3210 BP; 776 A; 813 C; 803 G; 818 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgac
gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
gcgaatttta acaaaatatt aacgtttaca atttcgccat tcgccattca ggctgcgcaa
ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg
gggatcctct agagccaccc ccctggtgca ggcatgcaag cttttgttcc ctttagtgag
ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
aaaataggcg tatcacgagg ccctttcgtc
//