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ID CARNEGIE2 preliminary; circular DNA; SYN; 3622 BP.
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AC M30841; ATCC37239;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Drosophila/E.coli plasmid vector Carnegie 2 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pCIW1 from pUC8 & p6.1
RC pCIW2 from pCIW1
RC pCIW3 from pCIW2
RC pCIW4 from pCIW3 & linker
RC pV9, pV10, pV11 from pCIW4
RC pV1 from pCIW4 & pUC8
RC Carnegie 1 from pCIW4 & pUC8
RC Carnegie 2 from Carnegie 1 & linker
RC Carnegie 3 from Carnegie 1 & linker
RC Carnegie 4 from Carnegie 1 & M13mp10
RC Carnegie 20 from Carnegie 2 & Drosophila rosy gene
RA Rubin G.M., Spradling A.C.;
RT "Vectors for P element-mediated gene transfer in Drosophila";
RL Nucleic Acids Res. 11:6341-6351(1983).
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CC Created by Moore, July 1995, under contract with NCBI.
CC This is a plasmid vector containing a non-autonomous P element from
CC Drosophila melanogaster. It should be used with a helper plasmid such
CC as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
CC Medium is 1227 LB plus ampicillin.
CC NM (Carnegie 2)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli K-12)(Drosophila melanogaster)(E.coli)(E.coli HB101)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (Carnegie 1)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. Carnegie 1 SmaI 3616bp 596..596, MCS
FT HpaI linker 6bp gttaac
FT -> Carnegie 2 3622bp"
FT - 1..595
FT /note="Carnegie 1 1..595 595bp
FT SmaI = CCC^GGG
FT HpaI = GTT^AAC"
FT - 596..601
FT /note="gttaac 6bp
FT HpaI = GTT^AAC
FT SmaI = CCC^GGG"
FT - 602..3622
FT /note="Carnegie 1 596..3616 3021bp"
FT misc_binding 0..0
FT /note="MCS HindIII-PstI-SalI-BamHI-HpaI-EcoRI"
FT misc_binding 0..0
FT /note="SIT unique HindIII-PstI-SalI-BamHI-HpaI-EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 3622 BP; 977 A; 858 C; 834 G; 953 T; 0 other;
catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcccgttaa
cggggatccg tcgacctgca gccaagcttt gcgtactcgc aaattattaa aaataaaact
ttaaaaataa tttcgtctaa ttaatattat gagttaattc aaaccccacg gacatgctaa
gggttaatca acaatcatat cgctgtctca ctcagactca atacgacact cagaatacta
ttcctttcac tcgcacttat tgcaagcata cgttaagtgg atgtctcttg ccgacgggac
caccttatgt tatttcatca tggtctggcc attctcatcg tgagcttccg ggtgctcgca
tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa
agctaatgta attatatttg tgccaataaa aacaagatat gacctataga atacaagtat
ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac
acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt
ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac
gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt
tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg
catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
ctcgtcaggg gggcggagcc tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc
agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
gctggactac gtgggtctgg cc
//