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ID CARNEGIE3 preliminary; circular DNA; SYN; 3624 BP.
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AC M30841; ATCC37240;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Drosophila/E.coli plasmid vector Carnegie 3 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pCIW1 from pUC8 & p6.1
RC pCIW2 from pCIW1
RC pCIW3 from pCIW2
RC pCIW4 from pCIW3 & linker
RC pV9, pV10, pV11 from pCIW4
RC pV1 from pCIW4 & pUC8
RC Carnegie 1 from pCIW4 & pUC8
RC Carnegie 2 from Carnegie 1 & linker
RC Carnegie 3 from Carnegie 1 & linker
RC Carnegie 4 from Carnegie 1 & M13mp10
RC Carnegie 20 or car20 from Carnegie 2 & Drosophila rosy gene
RA Rubin G.M., Spradling A.C.;
RT "Vectors for P element-mediated gene transfer in Drosophila";
RL Nucleic Acids Res. 11:6341-6351(1983).
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CC Created by Moore, July 1995, under contract with NCBI.
CC This is a plasmid vector containing a non-autonomous P element from
CC Drosophila melanogaster. It should be used with a helper plasmid such
CC as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
CC Medium is 1227 LB plus ampicillin.
CC NM (Carnegie 3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli K-12)(Drosophila melanogaster)(E.coli)(E.coli HB101)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (Carnegie 1)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. Carnegie 1 SmaI 3616bp 596..596, MCS
FT KpnI linker 8bp cggtaccg
FT -> Carnegie 3 3624bp"
FT - 1..595
FT /note="Carnegie 1 1..595 595bp
FT SmaI = CCC^GGG
FT KpnI = cGGTAC^Cg"
FT - 596..603
FT /note="cggtaccg 8bp
FT KpnI = cGGTAC^Cg
FT SmaI = CCC^GGG"
FT - 604..3624
FT /note="Carnegie 1 596..3616 3021bp"
FT misc_binding 0..0
FT /note="MCS HindIII-PstI-SalI-BamHI-KpnI-EcoRI"
FT misc_binding 0..0
FT /note="SIT unique HindIII-PstI-SalI-BamHI-KpnI-EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 3624 BP; 976 A; 860 C; 836 G; 952 T; 0 other;
catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttccccggta
ccgggggatc cgtcgacctg cagccaagct ttgcgtactc gcaaattatt aaaaataaaa
ctttaaaaat aatttcgtct aattaatatt atgagttaat tcaaacccca cggacatgct
aagggttaat caacaatcat atcgctgtct cactcagact caatacgaca ctcagaatac
tattcctttc actcgcactt attgcaagca tacgttaagt ggatgtctct tgccgacggg
accaccttat gttatttcat catggtctgg ccattctcat cgtgagcttc cgggtgctcg
catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
actgcttgtt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa
aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt
atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt
acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata
ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc
acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt
gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggtgcgg tatttcacac
cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
tgctcgtcag gggggcggag cctatggaaa aacgccttct tcttgaactc gggctcggtg
ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg
ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc
acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg
ccgctggact acgtgggtct ggcc
//