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ID CARNEGIE4 preliminary; circular DNA; SYN; 3631 BP.
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AC M30841; ATCC37241;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Drosophila/E.coli plasmid vector Carnegie 4 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pCIW1 from pUC8 & p6.1
RC pCIW2 from pCIW1
RC pCIW3 from pCIW2
RC pCIW4 from pCIW3 & linker
RC pV9, pV10, pV11 from pCIW4
RC pV1 from pCIW4 & pUC8
RC Carnegie 1 from pCIW4 & pUC8
RC Carnegie 2 from Carnegie 1 & linker
RC Carnegie 3 from Carnegie 1 & linker
RC Carnegie 4 from Carnegie 1 & M13mp10
RC Carnegie 20 or car20 from Carnegie 2 & Drosophila rosy gene
RA Rubin G.M., Spradling A.C.;
RT "Vectors for P element-mediated gene transfer in Drosophila";
RL Nucleic Acids Res. 11:6341-6351(1983).
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CC Created by Moore, July 1995, under contract with NCBI.
CC This is a plasmid vector containing a non-autonomous P element from
CC Drosophila melanogaster. It should be used with a helper plasmid such
CC as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
CC Medium is 1227 LB plus ampicillin.
CC NM (Carnegie 4)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli K-12)(Drosophila melanogaster)(E.coli)(E.coli HB101)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (Carnegie 1)(M13mp10)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. Carnegie 1 remove EcoRI-HindIII 30bp
FT \ 589..619, MCS/3586bp
FT 2. M13mp10 EcoRI-HindIII 45bp 6232..6277, MCS
FT -> Carnegie 4 3631bp"
FT - 1..588
FT /note="Carnegie 1 1..588 588bp
FT EcoRI = G^AATTC"
FT - 589..633
FT /note="M13mp10 6232..6276 45bp
FT HindIII = A^AGCTT"
FT - 634..3631
FT /note="Carnegie 1 619..3616 2998bp"
FT misc_binding 0..0
FT /note="MCS HindIII-PstI-SalI-XbaI-BamHI-SmaI-SstI-
FT EcoRI"
FT misc_binding 0..0
FT /note="SIT unique HindIII-PstI-SalI-XbaI-BamHI-SmaI-
FT SstI-EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 3631 BP; 978 A; 862 C; 837 G; 954 T; 0 other;
catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcgagctcg
cccggggatc ctctagagtc gacctgcagc ccaagctttg cgtactcgca aattattaaa
aataaaactt taaaaataat ttcgtctaat taatattatg agttaattca aaccccacgg
acatgctaag ggttaatcaa caatcatatc gctgtctcac tcagactcaa tacgacactc
agaatactat tcctttcact cgcacttatt gcaagcatac gttaagtgga tgtctcttgc
cgacgggacc accttatgtt atttcatcat ggtctggcca ttctcatcgt gagcttccgg
gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
cgaaataact gcttgttttt ttttttacca ttattaccat cgtgtttact gtttattgcc
ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa
tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa
aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg
ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca
ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc
accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggtgcggtat
ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccttcttct tgaactcggg
ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga
gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc
gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc
cgatctgccg ctggactacg tgggtctggc c
//