Return to this vector's summary.
ID COLE1 preliminary; circular DNA; PRO; 6646 BP.
XX
AC J01566; M33100;
XX
DT 04-AUG-1986 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector Colicin E1 (ColE1) - complete.
XX
KW cloning vector.
XX
OS Escherichia coli
OC Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
OC Enterobacteriaceae; Escherichia.
XX
RN [1]
RP 1008-1370
RC pNT1 or pVH51 from pML21, ColE1 ori
RA Tomizawa J.I., Ohmori H., Bird R.E.;
RT "Origin of replication of colicin E1 plasmid DNA";
RL Proc. Natl. Acad. Sci. U.S.A. 74:1865-1869(1977).
XX
RN [2]
RP 1410-1599
RC ColE1 from E. coli, relaxation site
RA Bastia D.;
RT "Determination of restriction sites and the nucleotide sequence
RT surrounding the relaxation site of ColE1";
RL J. Mol. Biol. 124:601-639(1978).
XX
RN [3]
RP 623-832
RC ColE1 from E. coli P678-54, colicin gene
RC pAO100 or pVH51 from pML21, mini-ColE1 segment
RC pAO2 [6] from pAO100
RA Morita M., Oka A.;
RT "The structure of a transcriptional unit on colicin E1 plasmid";
RL Eur. J. Biochem. 97:435-443(1979).
XX
RN [4]
RP 1-1613, 6577-6646
RC pAO2, pAO3 from ColE1, ori
RC pAO7 from pI258
RA Oka A., Nomura N., Morita M., Sugisaki H., Sugimoto K.,
RA Takanami M.;
RT "Nucleotide sequence of small ColE1 derivatives: structure of the
RT regions essential for autonomous replication and colicin E1
RT immunity";
RL Mol. Gen. Genet. 172:151-159(1979).
XX
RN [5]
RP 1-192, 384-519, 6644-6646
RC ColE1 from E. coli, initiation/termination
RA Patient R.K.;
RT "Characterization of in vitro transcription initiation and
RT termination sites in Col E1 DNA";
RL Nucleic Acids Res. 6:2647-2665(1979).
XX
RN [6]
RP 4783-5353
RC ColE1 from E. coli, colicin E1 gene promoter
RA Ebina Y., Kishi F., Miki T., Kagamiyama H., Nakazawa T.,
RA Nakazawa A.;
RT "The nucleotide sequence surrounding the promoter region of colicin
RT E1 gene";
RL Gene 15:119-126(1981).
XX
RN [7]
RP 639-753
RC ColE1 from E. coli, replication
RA Tomizawa J.I.;
RT "Control of ColE1 plasmid replication: the process of binding of RNA
RT I to the primer transcript";
RL Cell 38:861-870(1984).
XX
RN [8]
RP 5046-5110
RC ColE1 from E. coli, colicin E1 gene promoter
RA Parker R.C.;
RT "Mitomycin C-induced bidirectional transcription from the colicin E1
RT promoter region in plasmid ColE1";
RL Biochim. Biophys. Acta 868:39-44(1986).
XX
RN [9]
RP 57-123
RC pXT5 from ColE1 & pBR322
RC pSZ7 from pXT5
RA Zhiang S., Yan L., Zubay G.;
RT "Regulation of gene expression in plasmid ColE1: delayed expression
RT of the kil gene";
RL J. Bacteriol. 170:5460-5467(1988).
XX
RN [10]
RC ColE1 from E. coli, replication sites
RA Inoue N., Uchida H.;
RT "Transcription and initiation of ColE1 DNA replication in
RT Escherichia coli K-12";
RL J. Bacteriol. 173:1208-1214(1991).
XX
RN [11]
RC ColE1
RA Lebowitz J.;
RT ;
RL Submitted (04-APR-1985) to GenBank on computer-readable media by:
RL Lebowitz J., .
XX
RN [12]
RC ColE1 from E. coli, relaxation sites
RC ColE2 from E. coli, relaxation sites
RA Lovett M.A., Guiney D.G., Helinski D.R.;
RT "Relaxation complexes of plasmids ColE1 and ColE2: unique site of
RT the nick in the open circular DNA of the relaxed complexes";
RL Proc. Natl. Acad. Sci. U.S.A. 71:3854-3857(1974).
XX
CC ColE1 plasmid makes Colicin E1, which inhibits other E. coli
CC strains. Cells with the plasmid have immunity to Colicin E1.
CC This is the L (light) strand.
CC The open reading
CC frames noted by [8] are named in FEATURES by the molecular mass of
CC their predicted products or by the names given by [8].
CC NM (ColE1)(Colicin E1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (L)(light)
CC TY (plasmid)
CC SP (Sigma)
CC HO (E.coli C600)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (colicin E1)
CC BR ()
CC OF (ColE1Amp)
CC OR (2 bp 3' to EcoRI site)
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. E. coli, colicin gene
FT -> ColE1 6646bp"
FT mRNA complement(<59..409)
FT /note="RNA E. coli imm mRNA [4],[8]"
FT CDS complement(59..400)
FT /note="GEN E. coli colicin E1 immunity protein (imm)"
FT misc_binding 0..0
FT /note="SIT AvaI"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT misc_binding 0..0
FT /note="SIT SacII"
FT misc_binding 0..0
FT /note="SIT ScaI"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT CDS 448..585
FT /note="GEN E. coli kil protein gene"
FT misc_RNA 641..>641
FT /note="RNA E. coli RNAII"
FT misc_RNA complement(645..751)
FT /note="RNA E. coli RNAI (3' end +/- 1 bp) [5]"
FT rep_origin 1197..1197
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT (+/- 1 bp) [1]"
FT mRNA 1449..>3936
FT /note="RNA E. coli mob mRNA [4] [8];
FT 5' end putative"
FT misc_feature complement(1465..1466)
FT /note="SIT E. coli relaxation (nic) cut site [2]"
FT CDS 1609..1746
FT /note="GEN E. coli mob1 protein gene;
FT (GTG start codon)"
FT CDS complement(1612..1803)
FT /note="GEN E. coli RNAI inhibition modulator protein
FT gene; (rom - GTG start codon)"
FT mRNA complement(<3943..4887)
FT /note="RNA E. coli exc mRNA [4],[8]"
FT CDS complement(3943..4362)
FT /note="GEN E. coli entry exclusion protein 2 (exc2)"
FT CDS complement(4443..4862)
FT /note="GEN E. coli entry exclusion protein 1 (exc1)"
FT mRNA join(5056..6646,1..>585)
FT /note="RNA E. coli ColE1-kil mRNA [8]"
FT misc_binding 5063..5093
FT /note="SIT LexA binding site [8]"
FT mRNA join(5056..6646,1..>585)
FT /note="RNA E. coli ColE1-kil mRNA [8]"
FT CDS join(5140..6646,1..62)
FT /note="GEN E. coli colicin E1 protein (cea)"
XX
SQ Sequence 6646 BP; 1760 A; 1421 C; 1798 G; 1667 T; 0 other;
ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag
cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc
gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt
gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt
tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat
aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt
caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc
agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag
tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca
gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac
gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat
ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
aagagtgggt acatctgaag gagtgacaca gagtgacaga gctggaaaca catttgctga
acgccttaga gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct
tcgtggaatt gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc
gggtcatgca gttgagtcag caggtgcaac acttgagcga gcagacagaa cgcttgagcc
agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt
gttatcccta gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc
gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgc
agtttttagg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag
gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa
ttcttggctg tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc
tttgtcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt
gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg
ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt
tatcatggcg ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta
aagtatttat tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc
gttcagcgac cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc
ctgagctggg ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc
atatcctgtg cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagt
tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc
ctttctggtg tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca
aactcccgtc gtccgtccct gccggtacgg taactgacaa ggcctccgcg catatcacgg
taaaactgtg aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc
ataaagtgac agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt
ctgagaacgg taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt
gaaagactgt ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa
atgcagcggc gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat
ttttaactta ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac
tataaagatg gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt
actcctgacg ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt
tctgcagcta ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca
gagcaggctg cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg
gatgcgctga ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca
cgtacgcctt cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca
gagcgtttgc gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa
aaggcttttc aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca
gaacgccagt tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa
gctaaagctg ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa
atggatggag agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat
gcagaaatga aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat
aaagaactgg atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac
cgtccttttt ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa
caaaaacagg taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag
atccagaagg ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat
gaagctgaag aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat
gctgttgatg caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat
tcgaaaatgg cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat
gaagctctcg ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc
gatcgtgatg ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat
ttagatcagt ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg
gtatctgata tcctaaaaat taaggataca ggtgactgga agccactatt tcttacatta
gagaagaaag ctgcagatgc aggggtgagt tatgttgttg ctttactttt tagcttgctt
gctggaacta cattaggtat ttggggtatt gctattgtta caggaa
//