Return to this vector's summary.
ID EMBL3LFARM preliminary; circular DNA; SYN; 20067 BP.
XX
AC U02425; U02453; X58667; X58668; X06780; IG1003; ATCC37266;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector lambda EMBL3 left arm - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC from EMBL3
RC from EMBL4
RC from EMBL12
RA Frischauf A.M., Murray N.E., Lehrach H.;
RT "Lambda phage vectors - EMBL series";
RL Meth. Enzymol. 153:103-115(1987).
XX
RN [2]
RC EMBL1 from lambda 1059 & pBR322
RC EMBL2 from EMBL1
RC EMBL2Sal, EMBL2RI from EMBL2
RC [pUC2 from M13mp2]
RC pUC(S-Bg), pUC(R-Bg) from pUC2
RC EMBL3 from EMBL2Sal & EMBL2RI & pUC(S-Bg)
RC EMBL4 from EMBL2Sal & EMBL2RI & pUC(R-Bg)
RC EMBL3AamBam, EMBL3Sam7, EMBL3Aam, EMBL3Bam from EMBL3
RC EMBL3AamSam, EMBL3BamSam from EMBL3
RA Frischauf A.M., Lehrach H., Poustka A., Murray N.E.;
RT "Lambda replacement vectors carrying polylinker sequences";
RL J. Mol. Biol. 170:827-842(1983).
XX
RN [3]
RC from EMBL3
RC from EMBL4
RC from EMBL12
RA Karn J.M., Brenner S., Barnett L.;
RT "New bacteriophage lambda vectors with positive selection for
RT cloned inserts";
RL Meth. Enzymol. 101:3-19(1983).
XX
RN [4]
RC from EMBL3
RC from EMBL4
RC from EMBL12
RC from lambda gt series
RC from M13 series
RA Maniatis T., Fritsch E., Sambrook J.;
RT ;
RL Molecular Cloning 0:248-248(1989).
RL Maniatis T., Fritsch E., Sambrook J.;
RL 2nd Ed., Cold Spring Harbor Laboratory, New York.
XX
RN [5]
RC from EMBL3
RC from EMBL4
RC from EMBL12
RA Kaiser K., Murray N.E.;
RT "The use of phage lambda replacement vectors in the construction
RT of representative genomic DNA libraries";
RL DNA Cloning 1:1-47(1985).
RL Glover D.M., ed., IRL Press, Oxford.
XX
RN [6]
RC lambda from E. coli, gamma gene
RA Zissler J., Signer E., Schaefer F.;
RT "The role of recombination in growth of bacteriophage lambda I. the
RT gamma gene";
RL The Bacteriophage Lambda 0:455-468(1971).
RL Hershey A.D., ed., Cold Spring Harbor Laboratory, New York.
XX
RN [7]
RC from EMBL3, polylinker
RC from EMBL4, polylinker
RA Manninen I., Schulman A.H.;
RT "The lambda EMBL3 polylinker and surrounding region for PCR primers";
RL Biotechniques 14:174-174(1993).
XX
RN [8]
RC EMBL3, polylinker
RC EMBL4, polylinker
RA Goedert M., Rogers J., Wilson P.W.;
RT ;
RL Submitted (08-FEB-1988) to GenBank by:
RL Goedert M., Rogers J., Wilson P.W.,
RL Department of Physiology, University of Cambridge, Downing Street,
RL Cambridge CB2 3EG, UNITED KINGDOM.
XX
RN [9]
RC EMBL3, polylinker
RC EMBL4, polylinker
RA Schulman A.H.;
RT ;
RL Submitted (27-MAR-1991) to GenBank by:
RL Schulman A.H., Institute of Biotechnology, University of Helsinki,
RL Karvaamokuja 3, SF-00380 Helsinki, FINLAND.
XX
RN [10]
RP 1-20067, 1-9170
RC EMBL3 right/left arm
RA Kitts P.A.;
RT "CLONTECH Vectors On Disc version 1.1";
RL Unpublished (1993).
XX
RN [11]
RP 1-20067, 1-9170
RC EMBL3 right/left arm
RA Kitts P.A.;
RT ;
RL Submitted (07-OCT-1993) by:
RL Kitts P.A., CLONTECH Laboratories, Inc.,
RL 4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC EMBL3 size is between 43000 and 44000 bp.
CC left arm is 19300. right arm is 9200 bp. The stuffer is 14000 bp.
CC Phage with inserts have the Spi- phenotype. [3]
CC lambdaEMBL3 (ATCC 37266) and lambdaEMBL4 (ATCC 37267) differ only in
CC the orientation of the MCS surrounding the stuffer fragment.
CC Medium is 1592 SM buffer.
CC This sequence has been compiled from information in the sequence
CC databases, published literature and other sources; this vector has
CC not been completely sequenced. If you suspect there is an error in
CC this sequence, please contact CLONTECH's Technical Service
CC Department at (415) 424-8222 or (800) 662-2566, extension 3 or
CC E-mail CLONTECH@BIOTECHNET.COM.
CC NCBI gi: 413791
CC NCBI gi: 413819
CC NM (lambda EMBL3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)(linear)
CC ST ()
CC TY (phage)
CC SP (CLONTECH)(Promega)(Stratagene)(ATCC)
CC HO (E.coli LE392)(E.coli O358)(E.coli O359)(E.coli K803)
CC HO (E.coli NM538)(E.coli NM539)(E.coli KW251)
CC HO (E.coli XL-1-Blue MRA)(E.coli XL-1-Blue MRA P2)
CC CP ()
CC FN (cloning 8000-23000 bp)(chromosome walking)
CC SE ()
CC PA (lambda 1059)
CC BR (EMBL4)
CC OF ()
CC OR (E.coli Y1090)
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda 1059 remove middle HindIII-HindIII
FT \ 21011bp, lambda 23131..44142/27491bp
FT \ lambda 23131 25158 27480 36896 37460 44142
FT 2. E. coli HindIII-HindIII 5700bp, trpE gene
FT -> EMBL1 33200bp
FT 1. EMBL1 EcoRI, lambda 21227
FT \ lambda 21227 26105 31748 39169 44973
FT mutation
FT EcoRI, lambda 44973
FT mutation
FT -> EMBL2 33200bp [no EcoRI]
FT 1. M13mp2, MCS
FT -> pUC2
FT 1. pUC2 EcoRI 2200bp, pUC4 327 or 369
FT EcoRI-BglII-EcoRI linker 18bp gaattcagatctgaattc
FT EcoRI-EcoRI
FT -> pUC(R-Bg) 2200bp [EcoRI-BglII-EcoRI]
FT 1. pUC2 SalI 2200bp, pUC4 342 or 354
FT SalI-BglII-SalI linker 18bp gtcgacagatctgtcgac
FT SalI-SalI
FT -> pUC(S-Bg) 2200bp
FT \ [EcoRI-BamHI-SalI-BglII-SalI-BamHI-EcoRI]
FT 1. EMBL2 remove small BamHI-BamHI
FT \ 16841bp lambda 5506..22347/16359bp
FT \ lambda 5506 22347 27973 34500 41733
FT 2. pUC(R-Bg) BglII 2200bp
FT -> EMBLRI
FT 1. EMBL2 remove small BamHI-BamHI
FT \ 16841bp lambda 5506..22347/16359bp
FT \ lambda 5506 22347 27973 34500 41733
FT 2. pUC(S-Bg) BglII 2200bp
FT -> EMBLSal
FT 1. EMBLRI remove middle EcoRI-EcoRI 2200bp
FT 2. E. coli EcoRI-EcoRI
FT -> phage
FT 1. EMBLSal remove middle EcoRI-EcoRI 2200bp
FT 2. E. coli EcoRI-EcoRI
FT -> phage2
FT 1. EMBLRI middle EcoRI-EcoRI 2200bp
FT 2. phage2 remove middle EcoRI-EcoRI, 27000bp
FT -> EMBL3 29237bp
FT 1. EMBLSal middle EcoRI-EcoRI 2200bp
FT 2. phage remove middle EcoRI-EcoRI, 40800bp
FT -> EMBL4 43000bp"
FT misc_feature 0..0
FT /note="sequence to the left and right of polylinker"
FT misc_binding 0..0
FT /note="MCS double SalI-BamHI-EcoRI-EcoRI-BamHI-SalI
FT ggatctgggtcgacctgcaggtcaacggatccgttgacctgcaggtcgac
FT ccagatctgggtcgaccggtcgacccagatcc"
FT misc_binding 0..0
FT /note="SIT EcoRI-EcoRI, left and right arms,
FT ggatctgggtcgacctgcaggtcaacg gatccgttgacctgcaggtcgac
FT ccagatctgggtcgaccggtcgacccagatcc"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT misc_binding 0..0
FT /note="SIT KpnI"
FT misc_binding 0..0
FT /note="SIT KpnI"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT misc_feature 0..0
FT /note="lambda 2001 stuffer fragment (13700 bp)"
FT CDS 0..0
FT /note="GEN bacteriophage lambda red+"
FT CDS 0..0
FT /note="GEN bacteriophage lambda gam+"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT misc_binding 0..0
FT /note="SIT SalI"
FT misc_binding 0..0
FT /note="SIT SalI"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT misc_binding 0..0
FT /note="SIT SstI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT CDS 0..0
FT /note="GEN bacteriophage lambda Spi+ reporter gene"
XX
SQ Sequence 20067 BP; 4571 A; 5055 C; 6319 G; 4122 T; 0 other;
gggcggcgac ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg
ttcttcttcg tcataactta atgtttttat ttaaaatacc ctctgaaaag aaaggaaacg
acaggtgctg aaagcgaggc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt
ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggtg cgagtatccg
taccattcag aactggcagg aacagggaat gcccgttctg cgaggcggtg gcaagggtaa
tgaggtgctt tatgactctg ccgccgtcat aaaatggtat gccgaaaggg atgctgaaat
tgagaacgaa aagctgcgcc gggaggttga agaactgcgg caggccagcg aggcagatct
ccagccagga actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca
ggaactgaag aatgccagag actccgctga agtggtggaa accgcattct gtactttcgt
gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggctccccc tgtcggtgca
gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa
agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat
cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca
ggccggagcc acagaccgcc gttgaatggg cggatgctaa ttactatctc ccgaaagaat
ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga
tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca
aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac acccttatct
ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgactattc
gtgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac cgggataaca
cgctcaccat gaagcgtttc actaatgggc gtggcttctg gtgcctgggc ggtaaagcgg
caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt gctgcttttg
atgatgatat tgaacaggaa ggctctccga cgttcctggg tgacaagcgt attgaaggct
cggtctggcc aaagtccatc cgtggctcca cgccaaaagt gagaggcacc tgtcagattg
agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcattgcg
gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc aaatggacgc
cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgccagc
aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg
atggcattct ctggttttcg tcatccggtg aagagattga gccacctgac agtgtgacct
ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga
tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt aaacaccacg ctcggtgaga
cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag cggaaagagc
attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccagc
tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg
accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg
ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcgatatcc cgtatctgct
gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaaa catgggctgt
tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccggtggcc agcatgccac
gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc gcgaaagagc
agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc ggtgccgttc
acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg actgctgaag
agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc aaaaagcgac
gcaatgaggc actcgactgc ttcgtttatg cgctggcggc gctgcgcatc agtatttccc
gctggcagct ggatctcagt gcgctgctgg cgagcctgca ggaagaggat ggtgcagcaa
ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat gaatgacgcg
acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag gtaaacgggt
ggcaacagta cagaaagacg gacgaagggt ggagtttacg gccacttccg tgtctgacct
gaaaaaatat attgcagagc tggaagtgca gaccggcatg acacagcgac gcaggggacc
tgcaggattt tatgtatgaa aacgcccacc attcccaccc ttctggggcc ggacggcatg
acatcgctgc gcgaatatgc cggttatcac ggcggtggca gcggatttgg agggcagttg
cggtcgtgga acccaccgag tgaaagtgtg gatgcagccc tgttgcccaa ctttacccgt
ggcaatgccc gcgcagacga tctggtacgc aataacggct atgccgccaa cgccatccag
ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc
tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg
aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc
atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc
acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag
cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt
aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg
ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcctc gttcattcac
gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg
gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc cattgtgaag
gcgatgtatg ccgccaccat tgagagtgag ctggatacgc agtcagcgat ggattttatt
ctgggcgcga acagtcagga gcagcgggaa aggctgaccg gctggattgg tgaaattgcc
gcgtattacg ccgcagcgcc ggtccggctg ggaggcgcaa aagtaccgca cctgatgccg
ggtgactcac tgaacctgca gacggctcag gatacggata acggctactc cgtgtttgag
cagtcactgc tgcggtatat cgctgccggg ctgggtgtct cgtatgagca gctttcccgg
aattacgccc agatgagcta ctccacggca cgggccagtg cgaacgagtc gtgggcgtac
tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg
ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg cttcagtttt
caggaagccc gcagtgcctg ggggaactgc gactggatag gctccggtcg tatggccatc
gatggtctga aagaagttca ggaagcggtg atgctgatag aagccggact gagtacctac
gagaaagagt gcgcaaaacg cggtgacgac tatcaggaaa tttttgccca gcaggtccgt
gaaacgatgg agcgccgtgc agccggtctt aaaccgcccg cctgggcggc tgcagcattt
gaatccgggc tgcgacaatc aacagaggag gagaagagtg acagcagagc tgcgtaatct
cccgcatatt gccagcatgg cctttaatga gccgctgatg cttgaacccg cctatgcgcg
ggttttcttt tgtgcgcttg caggccagct tgggatcagc agcctgacgg atgcggtgtc
cggcgacagc ctgactgccc aggaggcact cgcgacgctg gcattatccg gtgatgatga
cggaccacga caggcccgca gttatcaggt catgaacggc atcgccgtgc tgccggtgtc
cggcacgctg gtcagccgga cgcgggcgct gcagccgtac tcggggatga ccggttacaa
cggcattatc gcccgtctgc aacaggctgc cagcgatccg atggtggacg gcattctgct
cgatatggac acgcccggcg ggatggtggc gggggcattt gactgcgctg acatcatcgc
ccgtgtgcgt gacataaaac cggtatgggc gcttgccaac gacatgaact gcagtgcagg
tcagttgctt gccagtgccg cctcccggcg tctggtcacg cagaccgccc ggacaggctc
catcggcgtc atgatggctc acagtaatta cggtgctgcg ctggagaaac agggtgtgga
aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca gccatcttcc
ggatgacgtc cgggagacac tgcagtcccg gatggacgca acccgccaga tgtttgcgca
gaaggtgtcg gcatataccg gcctgtccgt gcaggttgtg ctggataccg aggctgcagt
gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtta acagcaccga
tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa tcccgtctct caggagggcg
aatgaccaaa gagactcaat caacaactgt ttcagccact gcttcgcagg ctgacgttac
tgacgtggtg ccagcgacgg agggcgagaa cgccagcgcg gcgcagccgg acgtgaacgc
gcagatcacc gcagcggttg cggcagaaaa cagccgcatt atggcgatcc tcaactgtga
ggaggctcac ggacgcgaag aacaggcacg cgtgctggca gaaacccccg gtatgaccgt
gaaaacggcc cgccgcattc tggccgcagc accacagagt gcacaggcgc gcagtgacac
tgcgctggat cgtctgatgc agggggcacc ggcaccgctg gctgcaggta acccggcatc
tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga cgagcaaaga
aacctttacc cattaccagc cgcagggcaa cagtgacccg gctcataccg caaccgcgcc
cggcggattg agtgcgaaag cgcctgcaat gaccccgctg atgctggaca cctccagccg
taagctggtt gcgtgggatg gcaccaccga cggtgctgcc gttggcattc ttgcggttgc
tgctgaccag accagcacca cgctgacgtt ctacaagtcc ggcacgttcc gttatgagga
tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa cggaccgcgt ttgccggaac
ggcaatcagc atcgtttaac tttacccttc atcactaaag gccgcctgtg cggctttttt
tacgggattt ttttatgtcg atgtacacaa ccgcccaact gctggcggca aatgagcaga
aatttaagtt tgatccgctg tttctgcgtc tctttttccg tgagagctat cccttcacca
cggagaaagt ctatctctca caaattccgg gactggtaaa catggcgctg tacgtttcgc
cgattgtttc cggtgaggtt atccgttccc gtggcggctc cacctctgaa tttacgccgg
gatatgtcaa gccgaagcat gaagtgaatc cgcagatgac cctgcgtcgc ctgccggatg
aagatccgca gaatctggcg gacccggctt accgccgccg tcgcatcatc atgcagaaca
tgcgtgacga agagctggcc attgctcagg tcgaagagat gcaggcagtt tctgccgtgc
ttaagggcaa atacaccatg accggtgaag ccttcgatcc ggttgaggtg gatatgggcc
gcagtgagga gaataacatc acgcagtccg gcggcacgga gtggagcaag cgtgacaagt
ccacgtatga cccgaccgac gatatcgaag cctacgcgct gaacgccagc ggtgtggtga
atatcatcgt gttcgatccg aaaggctggg cgctgttccg ttccttcaaa gccgtcaagg
agaagctgga tacccgtcgt ggctctaatt ccgagctgga gacagcggtg aaagacctgg
gcaaagcggt gtcctataag gggatgtatg gcgatgtggc catcgtcgtg tattccggac
agtacgtgga aaacggcgtc aaaaagaact tcctgccgga caacacgatg gtgctgggga
acactcaggc acgcggtctg cgcacctatg gctgcattca ggatgcggac gcacagcgcg
aaggcattaa cgcctctgcc cgttacccga aaaactgggt gaccaccggc gatccggcgc
gtgagttcac catgattcag tcagcaccgc tgatgctgct ggctgaccct gatgagttcg
tgtccgtaca actggcgtaa tcatggccct tcggggccat tgtttctctg tggaggagtc
catgacgaaa gatgaactga ttgcccgtct ccgctcgctg ggtgaacaac tgaaccgtga
tgtcagcctg acggggacga aagaagaact ggcgctccgt gtggcagagc tgaaagagga
gcttgatgac acggatgaaa ctgccggtca ggacacccct ctcagccggg aaaatgtgct
gaccggacat gaaaatgagg tgggatcagc gcagccggat accgtgattc tggatacgtc
tgaactggtc acggtcgtgg cactggtgaa gctgcatact gatgcacttc acgccacgcg
ggatgaacct gtggcatttg tgctgccggg aacggcgttt cgtgtctctg ccggtgtggc
agccgaaatg acagagcgcg gcctggccag aatgcaataa cgggaggcgc tgtggctgat
ttcgataacc tgttcgatgc tgccattgcc cgcgccgatg aaacgatacg cgggtacatg
ggaacgtcag ccaccattac atccggtgag cagtcaggtg cggtgatacg tggtgttttt
gatgaccctg aaaatatcag ctatgccgga cagggcgtgc gcgttgaagg ctccagcccg
tccctgtttg tccggactga tgaggtgcgg cagctgcggc gtggagacac gctgaccatc
ggtgaggaaa atttctgggt agatcgggtt tcgccggatg atggcggaag ttgtcatctc
tggcttggac ggggcgtacc gcctgccgtt aaccgtcgcc gctgaaaggg ggatgtatgg
ccataaaagg tcttgagcag gccgttgaaa acctcagccg tatcagcaaa acggcggtgc
ctggtgccgc cgcaatggcc attaaccgcg ttgcttcatc cgcgatatcg cagtcggcgt
cacaggttgc ccgtgagaca aaggtacgcc ggaaactggt aaaggaaagg gccaggctga
aaagggccac ggtcaaaaat ccgcaggcca gaatcaaagt taaccggggg gatttgcccg
taatcaagct gggtaatgcg cgggttgtcc tttcgcgccg caggcgtcgt aaaaaggggc
agcgttcatc cctgaaaggt ggcggcagcg tgcttgtggt gggtaaccgt cgtattcccg
gcgcgtttat tcagcaactg aaaaatggcc ggtggcatgt catgcagcgt gtggctggga
aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggtgccgctg accacggcgt
ttaaacaaaa tattgagcgg atacggcgtg aacgtcttcc gaaagagctg ggctatgcgc
tgcagcatca actgaggatg gtaataaagc gatgaaacat actgaactcc gtgcagccgt
actggatgca ctggagaagc atgacaccgg ggcgacgttt tttgatggtc gccccgctgt
ttttgatgag gcggattttc cggcagttgc cgtttatctc accggcgctg aatacacggg
cgaagagctg gacagcgata cctggcaggc ggagctgcat atcgaagttt tcctgcctgc
tcaggtgccg gattcagagc tggatgcgtg gatggagtcc cggatttatc cggtgatgag
cgatatcccg gcactgtcag atttgatcac cagtatggtg gccagcggct atgactaccg
gcgcgacgat gatgcgggct tgtggagttc agccgatctg acttatgtca ttacctatga
aatgtgagga cgctatgcct gtaccaaatc ctacaatgcc ggtgaaaggt gccgggacca
ccctgtgggt ttataagggg agcggtgacc cttacgcgaa tccgctttca gacgttgact
ggtcgcgtct ggcaaaagtt aaagacctga cgcccggcga actgaccgct gagtcctatg
acgacagcta tctcgatgat gaagatgcag actggactgc gaccgggcag gggcagaaat
ctgccggaga taccagcttc acgctggcgt ggatgcccgg agagcagggg cagcaggcgc
tgctggcgtg gtttaatgaa ggcgataccc gtgcctataa aatccgcttc ccgaacggca
cggtcgatgt gttccgtggc tgggtcagca gtatcggtaa ggcggtgacg gcgaaggaag
tgatcacccg cacggtgaaa gtcaccaatg tgggacgtcc gtcgatggca gaagatcgca
gcacggtaac agcggcaacc ggcatgaccg tgacgcctgc cagcacctcg gtggtgaaag
ggcagagcac cacgctgacc gtggccttcc agccggaggg cgtaaccgac aagagctttc
gtgcggtgtc tgcggataaa acaaaagcca ccgtgtcggt cagtggtatg accatcaccg
tgaacggcgt tgctgcaggc aaggtcaaca ttccggttgt atccggtaat ggtgagtttg
ctgcggttgc agaaattacc gtcaccgcca gttaatccgg agagtcagcg atgttcctga
aaaccgaatc atttgaacat aacggtgtga ccgtcacgct ttctgaactg tcagccctgc
agcgcattga gcatctcgcc ctgatgaaac ggcaggcaga acaggcggag tcagacagca
accggaagtt tactgtggaa gacgccatca gaaccggcgc gtttctggtg gcgatgtccc
tgtggcataa ccatccgcag aagacgcaga tgccgtccat gaatgaagcc gttaaacaga
ttgagcagga agtgcttacc acctggccca cggaggcaat ttctcatgct gaaaacgtgg
tgtaccggct gtctggtatg tatgagtttg tggtgaataa tgcccctgaa cagacagagg
acgccgggcc cgcagagcct gtttctgcgg gaaagtgttc gacggtgagc tgagttttgc
cctgaaactg gcgcgtgaga tggggcgacc cgactggcgt gccatgcttg ccgggatgtc
atccacggag tatgccgact ggcaccgctt ttacagtacc cattattttc atgatgttct
gctggatatg cacttttccg ggctgacgta caccgtgctc agcctgtttt tcagcgatcc
ggatatgcat ccgctggatt tcagtctgct gaaccggcgc gaggctgacg aagagcctga
agatgatgtg ctgatgcaga aagcggcagg gcttgccgga ggtgtccgct ttggcccgga
cgggaatgaa gttatccccg cttccccgga tgtggcggac atgacggagg atgacgtaat
gctgatgaca gtatcagaag ggatcgcagg aggagtccgg tatggctgaa ccggtaggcg
atctggtcgt tgatttgagt ctggatgcgg ccagatttga cgagcagatg gccagagtca
ggcgtcattt ttctggtacg gaaagtgatg cgaaaaaaac agcggcagtc gttgaacagt
cgctgagccg acaggcgctg gctgcacaga aagcggggat ttccgtcggg cagtataaag
ccgccatgcg tatgctgcct gcacagttca ccgacgtggc cacgcagctt gcaggcgggc
aaagtccgtg gctgatcctg ctgcaacagg gggggcaggt gaaggactcc ttcggcggga
tgatccccat gttcaggggg cttgccggtg cgatcaccct gccgatggtg ggggccacct
cgctggcggt ggcgaccggt gcgctggcgt atgcctggta tcagggcaac tcaaccctgt
ccgatttcaa caaaacgctg gtcctttccg gcaatcaggc gggactgacg gcagatcgta
tgctggtcct gtccagagcc gggcaggcgg cagggctgac gtttaaccag accagcgagt
cactcagcgc actggttaag gcgggggtaa gcggtgaggc tcagattgcg tccatcagcc
agagtgtggc gcgtttctcc tctgcatccg gcgtggaggt ggacaaggtc gctgaagcct
tcgggaagct gaccacagac ccgacgtcgg ggctgacggc gatggctcgc cagttccata
acgtgtcggc ggagcagatt gcgtatgttg ctcagttgca gcgttccggc gatgaagccg
gggcattgca ggcggcgaac gaggccgcaa cgaaagggtt tgatgaccag acccgccgcc
tgaaagagaa catgggcacg ctggagacct gggcagacag gactgcgcgg gcattcaaat
ccatgtggga tgcggtgctg gatattggtc gtcctgatac cgcgcaggag atgctgatta
aggcagaggc tgcgtataag aaagcagacg acatctggaa tctgcgcaag gatgattatt
ttgttaacga tgaagcgcgg gcgcgttact gggatgatcg tgaaaaggcc cgtcttgcgc
ttgaagccgc ccgaaagaag gctgagcagc agactcaaca ggacaaaaat gcgcagcagc
agagcgatac cgaagcgtca cggctgaaat ataccgaaga ggcgcagaag gcttacgaac
ggctgcagac gccgctggag aaatataccg cccgtcagga agaactgaac aaggcactga
aagacgggaa aatcctgcag gcggattaca acacgctgat ggcggcggcg aaaaaggatt
atgaagcgac gctgaaaaag ccgaaacagt ccagcgtgaa ggtgtctgcg ggcgatcgtc
aggaagacag tgctcatgct gccctgctga cgcttcaggc agaactccgg acgctggaga
agcatgccgg agcaaatgag aaaatcagcc agcagcgccg ggatttgtgg aaggcggaga
gtcagttcgc ggtactggag gaggcggcgc aacgtcgcca gctgtctgca caggagaaat
ccctgctggc gcataaagat gagacgctgg agtacaaacg ccagctggct gcacttggcg
acaaggttac gtatcaggag cgcctgaacg cgctggcgca gcaggcggat aaattcgcac
agcagcaacg ggcaaaacgg gccgccattg atgcgaaaag ccgggggctg actgaccggc
aggcagaacg ggaagccacg gaacagcgcc tgaaggaaca gtatggcgat aatccgctgg
cgctgaataa cgtcatgtca gagcagaaaa agacctgggc ggctgaagac cagcttcgcg
ggaactggat ggcaggcctg aagtccggct ggagtgagtg ggaagagagc gccacggaca
gtatgtcgca ggtaaaaagt gcagccacgc agacctttga tggtattgca cagaatatgg
cggcgatgct gaccggcagt gagcagaact ggcgcagctt cacccgttcc gtgctgtcca
tgatgacaga aattctgctt aagcaggcaa tggtggggat tgtcgggagt atcggcagcg
ccattggcgg ggctgttggt ggcggcgcat ccgcgtcagg cggtacagcc attcaggccg
ctgcggcgaa attccatttt gcaaccggag gatttacggg aaccggcggc aaatatgagc
cagcggggat tgttcaccgt ggtgagtttg tcttcacgaa ggaggcaacc agccggattg
gcgtggggaa tctttaccgg ctgatgcgcg gctatgccac cggcggttat gtcggtacac
cgggcagcat ggcagacagc cggtcgcagg cgtccgggac gtttgagcag aataaccatg
tggtgattaa caacgacggc acgaacgggc agataggtcc ggctgctctg aaggcggtgt
atgacatggc ccgcaagggt gcccgtgatg aaattcagac acagatgcgt gatggtggcc
tgttctccgg aggtggacga tgaagacctt ccgctggaaa gtgaaacccg gtatggatgt
ggcttcggtc ccttctgtaa gaaaggtgcg ctttggtgat ggctattctc agcgagcgcc
tgccgggctg aatgccaacc tgaaaacgta cagcgtgacg ctttctgtcc cccgtgagga
ggccacggta ctggagtcgt ttctggaaga gcacgggggc tggaaatcct ttctgtggac
gccgccttat gagtggcggc agataaaggt gacctgcgca aaatggtcgt cgcgggtcag
tatgctgcgt gttgagttca gcgcagagtt tgaacaggtg gtgaactgat gcaggatatc
cggcaggaaa cactgaatga atgcacccgt gcggagcagt cggccagcgt ggtgctctgg
gaaatcgacc tgacagaggt cggtggagaa cgttattttt tctgtaatga gcagaacgaa
aaaggtgagc cggtcacctg gcaggggcga cagtatcagc cgtatcccat tcaggggagc
ggttttgaac tgaatggcaa aggcaccagt acgcgcccca cgctgacggt ttctaacctg
tacggtatgg tcaccgggat ggcggaagat atgcagagtc tggtcggcgg aacggtggtc
cggcgtaagg tttacgcccg ttttctggat gcggtgaact tcgtcaacgg aaacagttac
gccgatccgg agcaggaggt gatcagccgc tggcgcattg agcagtgcag cgaactgagc
gcggtgagtg cctcctttgt actgtccacg ccgacggaaa cggatggcgc tgtttttccg
ggacgtatca tgctggccaa cacctgcacc tggacctatc gcggtgacga gtgcggttat
agcggtccgg ctgtcgcgga tgaatatgac cagccaacgt ccgatatcac gaaggataaa
tgcagcaaat gcctgagcgg ttgtaagttc cgcaataacg tcggcaactt tggcggcttc
ctttccatta acaaactttc gcagtaaatc ccatgacaca gacagaatca gcgattctgg
cgcacgcccg gcgatgtgcg ccagcggagt cgtgcggctt cgtggtaagc acgccggagg
gggaaagata tttcccctgc gtgaatatct ccggtgagcc ggaggctatt tccgtatgtc
gccggaagac tggctgcagg cagaaatgca gggtgagatt gtggcgctgg tccacagcca
ccccggtggt ctgccctggc tgagtgaggc cgaccggcgg ctgcaggtgc agagtgattt
gccgtggtgg ctggtctgcc gggggacgat tcataagttc cgctgtgtgc cgcatctcac
cgggcggcgc tttgagcacg gtgtgacgga ctgttacaca ctgttccggg atgcttatca
tctggcgggg attgagatgc cggactttca tcgtgaggat gactggtggc gtaacggcca
gaatctctat ctggataatc tggaggcgac ggggctgtat caggtgccgt tgtcagcggc
acagccgggc gatgtgctgc tgtgctgttt tggttcatca gtgccgaatc acgccgcaat
ttactgcggc gacggcgagc tgctgcacca tattcctgaa caactgagca aacgagagag
gtacaccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg catggcgcgc
atctgccttt acggggattt acaacgattt ggtcgccgca tcgaccttcg tgtgaaaacg
ggggctgaag ccatccgggc actggccaca cagctcccgg cgtttcgtca gaaactgagc
gacggctggt atcaggtacg gattgccggg cgggacgtca gcacgtccgg gttaacggcg
cagttacatg agactctgcc tgatggcgct gtaattcata ttgttcccag agtcgccggg
gccaagtcag gtggcgtatt ccagattgtc ctgggggctg ccgccattgc cggatcattc
tttaccgccg gagccaccct tgcagcatgg ggggcagcca ttggggccgg tggtatgacc
ggcatcctgt tttctctcgg tgccagtatg gtgctcggtg gtgtggcgca gatgctggca
ccgaaagcca gaactccccg tatacagaca acggataacg gtaagcagaa cacctatttc
tcctcactgg ataacatggt tgcccagggc aatgttctgc ctgttctgta cggggaaatg
cgcgtggggt cacgcgtggt ttctcaggag atcagcacgg cagacgaagg ggacggtggt
caggttgtgg tgattggtcg ctgatgcaaa atgttttatg tgaaaccgcc tgcgggcggt
tttgtcattt atggagcgtg aggaatgggt aaaggaagca gtaaggggca taccccgcgc
gaagcgaagg acaacctgaa gtccacgcag ttgctgagtg tgatcgatgc catcagcgaa
gggccgattg aaggtccggt ggatggctta aaaagcgtgc tgctgaacag tacgccggtg
ctggacactg aggggaatac caacatatcc ggtgtcacgg tggtgttccg ggctggtgag
caggagcaga ctccgccgga gggatttgaa tcctccggct ccgagacggt gctgggtacg
gaagtgaaat atgacacgcc gatcacccgc accattacgt ctgcaaacat cgaccgtctg
cgctttacct tcggtgtaca ggcactggtg gaaaccacct caaagggtga caggaatccg
tcggaagtcc gcctgctggt tcagatacaa cgtaacggtg gctgggtgac ggaaaaagac
atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtgat gggtaacctg
ccgccgcgcc cgtttaatat ccggatgcgc aggatgacgc cggacagcac cacagaccag
ctgcagaaca aaacgctctg gtcgtcatac actgaaatca tcgatgtgaa acagtgctac
ccgaacacgg cactggtcgg cgtgcaggtg gactcggagc agttcggcag ccagcaggtg
agccgtaatt atcatctgcg cgggcgtatt ctgcaggtgc cgtcgaacta taacccgcag
acgcggcaat acagcggtat ctgggacgga acgtttaaac cggcatacag caacaacatg
gcctggtgtc tgtgggatat gctgacccat ccgcgctacg gcatggggaa acgtcttggt
gcggcggatg tggataaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg
ccggacggct ttggcggcac ggagccgcgc atcacctgta atgcgtacct gaccacacag
cgtaaggcgt gggatgtgct cagcgatttc tgctcggcga tgcgctgtat gccggtatgg
aacgggcaga cgctgacgtt cgtgcaggac cgaccgtcgg ataagacgtg gacctataac
cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
aaggaccgcc ataatgccgt tgaggtgaac tggattgacc cgaacaacgg ctgggagacg
gcgacagagc ttgttgaaga tacgcaggcc attgcccgtt acggtcgtaa tgttacgaag
atggatgcct ttggctgtac cagccggggg caggcacacc gcgccgggct gtggctgatt
aaaacagaac tgctggaaac gcagaccgtg gatttcagcg tcggcgcaga agggcttcgc
catgtaccgg gcgatgttat tgaaatctgc gatgatgact atgccggtat cagcaccggt
ggtcgtgtgc tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg
ctgccatcct ccggtaccgc gctgataagc ctggttgacg gaagtggcaa tccggtcagc
gtggaggttc agtccgtcac cgacggcgtg aaggtaaaag tgagccgtgt tcctgacggt
gttgctgaat acagcgtatg ggagctgaag ctgccgacgc tgcgccagcg actgttccgc
tgcgtgagta tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg
ccggaaaaag aggccatcgt ggataacggg gcgcactttg acggcgaaca gagtggcacg
gtgaatggtg tcacgccgcc agcggtgcag cacctgaccg cagaagtcac tgcagacagc
ggggaatatc aggtgctggc gcgatgggac acaccgaagg tggtgaaggg cgtgagtttc
ctgctccgtc tgaccgtaac agcggacgac ggcagtgagc ggctggtcag cacggcccgg
acgacggaaa ccacataccg cttcacgcaa ctggcgctgg ggaactacag gctgacagtc
cgggcggtaa atgcgtgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcc
gcaccggcag caccgtcgag gattgagctg acgccgggct attttcagat aaccgccacg
ccgcatcttg ccgtttatga cccgacggta cagtttgagt tctggttctc ggaaaagcag
attgcggata tcagacaggt tgaaaccagc acgcgttatc ttggtacggc gctgtactgg
atagccgcca gtatcaatat caaaccgggc catgattatt acttttatat ccgcagtgtg
aacaccgttg gcaaatcggc attcgtggag gccgtcggtc gggcgagcga tgatgcggaa
ggttacctgg attttttcaa aggcaagata accgaatccc atctcggcaa ggagctgctg
gaaaaagtcg agctgacgga ggataacgcc agcagactgg aggagttttc gaaagagtgg
aaggatgcca gtgataagtg gaatgccatg tgggctgtca aaattgagca gaccaaagac
ggcaaacatt atgtcgcggg tattggcctc agcatggagg acacggagga aggcaaactg
agccagtttc tggttgccgc caatcgtatc gcatttattg acccggcaaa cgggaatgaa
acgccgatgt ttgtggcgca gggcaaccag atattcatga acgacgtgtt cctgaagcgc
ctgacggccc ccaccattac cagcggcggc aatcctccgg ccttttccct gacaccggac
ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa ctccgggacg
ctcagtaatg tgacgatagc tgaaaactgt acgataaacg gtacgctgag ggcggaaaaa
atcgtcgggg acattgtaaa ggcggcgagc gcggcttttc cgcgccagcg tgaaagcagt
gtggactggc cgtcaggtac ccgtactgtc accgtgaccg atgaccatcc ttttgatcgc
cagatagtgg tgcttccgct gacgtttcgc ggaagtaagc gtactgtcag cggcaggaca
acgtattcga tgtgttatct gaaagtactg atgaacggtg cggtgattta tgatggcgcg
gcgaacgagg cggtacaggt gttctcccgt attgttgaca tgccagcggg tcggggaaac
gtgatcctga cgttcacgct tacgtccaca cggcattcgg cagatattcc gccgtatacg
tttgccagcg atgtgcaggt tatggtgatt aagaaacagg cgctgggcat cagcgtggtc
tgagtgtgtt acagaggttc gtccgggaac gggcgtttta ttataaaaca gtgagaggtg
aacgatgcgt aatgtgtgta ttgccgttgc tgtctttgcc gcacttgcgg tgacagtcac
tccggcccgt gcggaaggtg gacatggtac gtttacggtg ggctattttc aagtgaaacc
gggtacattg ccgtcgttgt cgggcgggga taccggtgtg agtcatctga aagggattaa
cgtgaagtac cgttatgagc tgacggacag tgtgggggtg atggcttccc tggggttcgc
cgcgtcgaaa aagagcagca cagtgatgac cggggaggat acgtttcact atgagagcct
gcgtggacgt tatgtgagcg tgatggccgg accggtttta caaatcagta agcaggtcag
tgcgtacgcc atggccggag tggctcacag tcggtggtcc ggcagtacaa tggattaccg
taagacggaa atcactcccg ggtatatgaa agagacgacc actgccaggg acgaaagtgc
aatgcggcat acctcagtgg cgtggagtgc aggtatacag attaatccgg cagcgtccgt
cgttgttgat attgcttatg aaggctccgg cagtggcgac tggcgtactg acggattcat
cgttggggtc ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg
gaaccggtgg gcttttttgt ggggtgaata tggcagtaaa gatttcagga gtcctgaaag
acggcacagg aaaaccggta cagaactgca ccattcagct gaaagccaga cgtaacagca
ccacggtggt ggtgaacacg gtgggctcag agaatccgga tgaagccgct tttttatact
aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca
gtcaaaataa aatcattatt tgatttcaat tttgtcccac tccctgcctc tgtcatcacg
atactgtgat gccatggtgt ccgacttatg cccgagaaga tgttgagcaa acttatcgct
tatctgcttc tcatagagtc ttgcagacaa actgcgcaac tcgtgaaagg taggcggatc
tgggtcgacc tgcaggtcaa cggatcc
//