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ID FKN16 preliminary; DNA; SYN; 8676 BP.
XX
AC ATCC37002;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector fd fKN 16 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC fKN16 from fd-tet
RC [fBH16 from fKN16 & linker]
RA Nelson F.K., Friedman S.M., Smith G.P.;
RT "Filamentous phage DNA cloning vectors: a noninfective mutant with
RT a nonpolar deletion in gene III";
RL Virology 108:338-350(1981).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC Provided as lyophilized viral DNA. When reconstituted with 50 ul of
CC sterile distilled water, the DNA concentration is about 10x10(11)
CC ssDNA molecules/ml in 1% SDS, 10 mM Tris pH8, 10 mM HCl, 1 mM EDTA.
CC (ATCC staff)
CC This vector contains a deletion in the phage gene III. It exhibits a
CC higher level of biological containment than fd-tet (ATCC 37000).
CC (ATCC staff)
CC fd bacteriophage vector. (ATCC staff)
CC Medium is 1592 SM buffer.
CC NM (fKN 16)
CC CM (yes)
CC NA (filamentous ss-DNA)
CC TP (linear)
CC ST ()
CC TY (phage)
CC SP (ATCC)
CC HO (bacteriophage fd)(E.coli K802)(E.coli C600)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (fd-tet)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. fd-tet remove Sau3A/MboI-BamHI 507bp
FT \ 2341..2848, gene III/9100bp
FT -> fKN16 9100bp"
FT - 1..2340
FT /note="fd-tet 1..2340 2340bp
FT MboI = Sau3AI = ^GATC
FT BamHI = G^GATCC"
FT - 2341..8676
FT /note="fd-tet 2848..9183 6336bp"
FT misc_binding 0..0
FT /note="SIT unique HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage fd"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ Sequence 8676 BP; 2189 A; 1704 C; 1773 G; 3010 T; 0 other;
aatagtggac tcttgttcca aactggaaca acactcacaa ctaactcggc ctattctttt
gatttataag gatttttgtc attttctgct tactggttaa aaaataagct gatttaacaa
atatttaacg cgaaatttaa caaaacatta acgtttacaa tttaaatatt tgcttataca
atcatcctgt ttttggggct tttctgatta tcaaccgggg tacatatgat tgacatgcta
gttttacgat taccgttcat cgattctctt gtttgctcca gactttcagg taatgacctg
atagcctttg tagacctctc aaaaatagct accctctccg gcatgaattt atcagctaga
acggttgaat atcatattga cggtgatttg actgtctccg gcctttctca cccgtttgaa
tctttgccta ctcattactc cggcattgca tttaaaatat atgagggttc taaaaatttt
tatccctgcg ttgaaattaa ggcttcacca gcaaaagtat tacagggtca taatgttttt
ggtacaaccg atttagcttt atgctctgag gctttattgc ttaattttgc taactctctg
ccttgcttgt acgatttatt ggatgttaac gctactacca ttagtagaat tgatgccacc
ttttcagctc gcgccccaaa tgaaaatata gctaaacagg ttattgacca tttgcgaaat
gtatctaatg gtcaaactaa atctactcgt tcgcagaatt gggaatcaac tgttacatgg
aatgaaactt ccagacaccg tactttagtt gcatatttaa aacatgttga actacagcac
cagattcagc aattaagctc taagccatcc gcaaaaatga cctcttatca aaaggagcaa
ttaaaggtac tgtctaatcc tgacctgttg gaatttgctt ccggtctggt tcgctttgag
gctcgaattg aaacgcgata tttgaagtct ttcgggcttc ctcttaatct ttttgatgca
attcgctttg cttctgacta taatagacag ggtaaagacc tgatttttga tttatggtca
ttctcgtttt ctgaactgtt taaagcattt gagggggatt caatgaatat ttatgacgat
tccgcagtat tggacgctat ccagtctaaa cattttacaa ttaccccctc tggcaaaact
tcctttgcaa aagcctctcg ctattttggt ttctatcgtc gtctggttaa tgagggttat
gatagtgttg ctcttaccat gcctcgtaat tccttttggc gttatgtatc tgcattagtt
gagtgtggta ttcctaaatc tcaattgatg aatctttcca cctgtaataa tgttgttccg
ttagttcgtt ttattaacgt agatttttcc tcccaacgtc ctgactggta taatgagcca
gttcttaaaa tcgcataagg taattcaaaa tgattaaagt tgaaattaaa ccgtctcaag
cgcaatttac tacccgttct ggtgtttctc gtcagggcaa gccttattca ctgaatgagc
agctttgtta cgttgatttg ggtaatgaat atccggtgct tgtcaagatt actctcgacg
aaggtcagcc agcgtatgcg cctggtctgt acaccgtgca tctgtcctcg ttcaaagttg
gtcagttcgg ttctcttatg attgaccgtc tgcgcctcgt tccggctaag taacatggag
caggtcgcgg atttcgacac aatttatcag gcgatgatac aaatctccgt tgtactttgt
ttcgcgcttg gtataatcgc tgggggtcaa agatgagtgt tttagtgtat tctttcgcct
ctttcgtttt aggttggtgc cttcgtagtg gcattacgta ttttacccgt ttaatggaaa
cttcctcatg aaaaagtctt tagtcctcaa agcctccgta gccgttgcta ccctcgttcc
gatgctgtct ttcgctgctg agggtgacga tcccgcaaaa gcggcctttg actccctgca
agcctcagcg accgaatata tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc
aactatcggt atcaagctgt ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac
aattaaaggc tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta
ttcgcaattc ctttagttgt tcctttctat tctcactccg ctgaaactgt tgaaagttgt
ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga caaaacttta
gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt
ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
ttaatgaata atttccgtca atatttacct tctttgcctc agtcggttga atgtcgccct
tatgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttcgacg
tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttactttcc
ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
ggcttaactc aattcttgtg ggttatctct ctgatattag cgcacaatta ccctctgatt
ttgttcaggg cgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
attgggataa ataaatatgg ctgtttattt tgtaactggc aaattaggct ctggaaagac
gctcgttagc gttggtaaga ttcaggataa aattgtagct gggtgcaaaa tagcaactaa
tcttgattta aggcttcaaa acctcccgca agtcgggagg ttcgctaaaa cgcctcgcgt
tcttagaata ccggataagc cttctatttc tgatttgctt gctattggtc gtggtaatga
ttcctacgac gaaaataaaa acggtttgct tgttcttgat gaatgcggta cttggtttaa
tacccgttca tggaatgaca aggaaagaca gccgattatt gattggtttc ttcatgctcg
taaattggga tgggatatta tttttcttgt tcaggattta tctattgttg ataaacaggc
gcgttctgca ttagctgaac acgttgttta ttgtcgccgt ctggacagaa ttactttacc
ctttgtcggc actttatatt ctcttgttac tggctcaaaa atgcctctgc ctaaattaca
tgttggtgtt gttaaatatg gtgattctca attaagccct actgttgagc gttggcttta
tactggtaag aatttatata acgcatatga cactaaacag gctttttcca gtaattatga
ttcaggtgtt tattcatatt taacccctta tttatcacac ggtcggtatt tcaaaccatt
aaatttaggt cagaagatga aattaactaa aatatatttg aaaaagtttt ctcgcgttct
ttgtcttgcg ataggatttg catcagcatt tacatatagt tatataaccc aacctaagcc
ggaggttaaa aaggtagtct ctcagaccta tgattttgat aaattcacta ttgactcttc
tcagcgtctt aatctaagct atcgctatgt tttcaaggat tctaagggaa aattaattaa
tagcgacgat ttacagaagc aaggttattc catcacatat attgatttat gtactgtttc
aattaaaaaa ggtaattcaa atgaaattgt taaatgtaat taattttgtt ttcttgatgt
ttgtttcatc atcttctttt gctcaagtaa ttgaaatgaa taattcgcct ctgcgcgatt
tcgtgacttg gtattcaaag caaacaggtg aatctgttat tgtctcacct gatgttaaag
gtacagtgac tgtatattcc tctgacgtta agcctgaaaa tttacgcaat ttctttatct
ctgttttacg tgctaataat tttgatatgg ttggctcaat tccttccata attcagaaat
ataacccaaa tagtcaggat tatattgatg aattgccatc atctgatatt caggaatatg
atgataattc cgctccttct ggtggtttct ttgttccgca aaatgataat gttactcaaa
catttaaaat taataacgtt cgcgcaaagg atttaataag ggttgtagaa ttgtttgtta
aatctaatac atctaaatcc tcaaatgtat tatctgttga tggttctaac ttattagtag
ttagcgcccc taaagatatt ttagataacc ttccgcaatt tctttctact gttgatttgc
caactgacca gatattgatt gaaggattaa ttttcgaggt tcagcaaggt gatgctttag
atttttcctt tgctgctggc tctcagcgcg gcactgttgc tggtggtgtt aatactgacc
gtctaacctc tgttttatct tctgcgggtg gttcgttcgg tatttttaac ggcgatgttt
tagggctatc agttcgcgca ttaaagacta atagccattc aaaaatattg tctgtgcctc
gtattcttac gctttcaggt cagaagggtt ctatttctgt tggccagaat gtccctttta
ttactggtcg tgtaactggt gaatctgcca atgtaaataa tccatttcag acggttgagc
gtcaaaatgt tggtatttct atgagtgttt ttcccgttgc aatggctggc ggtaatattg
ttttagatat aaccagtaag gccgatagtt tgagttcttc tactcaggca agtgatgtta
ttactaatca aagaagtatt gcgacaacgg ttaatttgcg tgatggtcag actcttttgc
tcggtggcct cactgattac aaaaacactt ctcaagattc tggtgtgccg ttcctgtcta
aaatcccttt aatcggcctc ctgtttagct cccgttctga ttctaacgag gaaagcacgt
tgtacgtgct cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
ttcgctttct tcccttcctt tctcgccacg ttctccggct ttccccgtca agctctaaat
cgggggatct atgattccct ttgtcaacag caatggatca ctgaaaatgg ttcaatgatc
acattaagtg gtattcaata ttttcatgaa atgggaattg acgttccttc caaacattca
cgtaaaatct gttgtgcgtg tttagattgg agtgaacgcc gtttccattt aggtgggtac
gttggagccg cattattttc gctttatgaa tctaaagggt ggttaactcg acatcttggt
taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt ttaagaccca ctttcacatt
taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg
caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag
taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta
aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt
ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac
ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt
tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc
tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat
acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga
ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag
acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc
cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat tggtaattac
gttactcgat gccatgggga ttggccttat catgccagtc ttgccaacgt tattacgtga
atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt
aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc
agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg ctttttcaag
tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag ctactggggc
tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga agtggttcgg
ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg gtggttttgc
aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata ttgtcacttt
ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata cagataccga
agtaggggtt gagacgcaat cgaattcggt atacatcact ttatttaaaa cgatgcccat
tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa cggtgtgggt
gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt cattagcggg
tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag ccactaaatg
gggcgaaaaa acggcagtac tgctcgaatt tattgcagat agtagtgcat ttgccttttt
agcgtttata tctgaaggtt ggttagattt ccctgtttta attttattgg ctggtggtgg
gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc atgagcaagg
tgctttacag ggattattgg tgagccttac caatgcaacc ggtgttattg gcccattact
gtttactgtt atttataatc attcactacc aatttgggat ggctggattt ggattattgg
tttagcgttt tactgtatta ttatcctgct atcgatgacc ttcatgttaa cccctcaagc
tcaggggagt aaacaggaga caagtgctta gttatttcgt caccaaatga tgttattccg
cgaaatataa tgaccctctt gataacccaa gagggcattt tttacgataa agaagattta
gcttcaaata aaacctatct attttattta tctttcaagc tcaataaaaa gccgcggtaa
atagcaataa attggccttt tttatcggca agctctttta ggtttttcgc atgtattgcg
atatgcataa accagccatt gagtaagttt ttaagcacat cactatcata agctttaagt
tggttctctt ggatcaattt gctgacaatg gcgtttacct taccagtaat gtattcaagg
ctaatttttt caagttcatt ccaaccaatg ataggcatca cttcttggat agggataagg
tttttattat tatcaataat ataatcaaga taatgttcaa atatactttc taaggcagac
caaccatttg ttaaatcagt ttttgttgtg atgtaggcat caatcataat taattgctgc
ttataacagg cactgagtaa ttgtttttta tttttaaagt gatgataaaa ggcacctttg
gtcaccaacg cttttcccga gatcccttta gggttccgat ttagtgcttt acggcacctc
gacctccaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
gtttttcgcc ctttgacgtt ggagtccacg ttcttt
//