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ID LNCX preliminary; circular DNA; SYN; 6620 BP.
XX
AC M28247;
XX
DT 28-JAN-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Mouse Moloney murine sarcoma virus vector LNCX - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-6620
RC LN, LNSX, LNCX, LXSN from LNL6
RA Miller A.D.;
RT ;
RL Unpublished (1989).
XX
RN [2]
RC LN, LNSX, LNCX, LXSN from LNL6
RA Miller A.D., Rosman G.J.;
RT "Improved retroviral vectors for gene transfer and expression";
RL Biotechniques 7:980-990(1989).
XX
RN [3]
RP 1-6620
RC LNCX
RA Miller A.D.;
RT ;
RL Submitted (22-SEP-1989) to GenBank on computer-readable media by:
RL Miller A.D., Program in Molecular Medicine,
RL Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
XX
RN [4]
RC LN, LNSX, LNCX, LXSN from LNL6
RA Kuebbing D., Freidman S.;
RT ;
RL Unpublished (1991).
XX
CC NCBI gi: 208846
CC NM (LNCX)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (mouse)
CC CP ()
CC FN (gene transfer)(expression)
CC SE ()
CC PA (LNL6)
CC BR (LN)(LNSX)(LXSN)
CC OF ()
CC OR (1 bp 5' to EcoRI site)
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. LNL6 remove 681bp,
FT \ MMLV LTR/packaging/neo/LTR/polyA/5464bp
FT 2. CMV 1138bp, immediate early promoter
FT 3. linker HindIII-HpaI-ClaI 18bp aagcttgttaacatcgat
FT -> LNCX 6620bp"
FT misc_recomb 144..145
FT /note="mouse DNA end/Moloney murine sarcoma virus
FT (Mo-MuSV) DNA start"
FT LTR 145..733
FT /note="RPT Moloney murine sarcoma virus (Mo-MuSV)
FT 5' long terminal repeat"
FT misc_signal 803..1612
FT /note="extended packaging signal"
FT misc_recomb 1133..1134
FT /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT mutation 1193..1195
FT /note="GAG atg start codon to TAG stop codon"
FT misc_recomb 1616..1617
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/transposon Tn5 DNA start"
FT CDS 1656..2450
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)"
FT misc_recomb 2799..2800
FT /note="transposon Tn5 DNA end/human cytomegalovirus
FT DNA start"
FT promoter 2800..3617
FT /note="PRO human cytomegalovirus immediate early gene"
FT misc_recomb 3617..3618
FT /note="human cytomegalovirus DNA end/Moloney murine
FT leukemia virus (Mo-MuLV) DNA start"
FT LTR 3666..4259
FT /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT 3' long terminal repeat"
FT misc_recomb 4328..4329
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/plasmid pBR322 DNA start"
XX
SQ Sequence 6620 BP; 1528 A; 1807 C; 1692 G; 1593 T; 0 other;
gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
ggagtgggga ggcacgatgg ccgctttggt cgaggcggat ccggccatta gccatattat
tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatccat
atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
ggcatgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
gcggccccaa gcttgttaac atcgataaaa taaaagattt tatttagtct ccagaaaaag
gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
cgaggccctt tcgtcttcaa
//