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ID LNL6 preliminary; circular DNA; SYN; 6145 BP.
XX
AC M63653;
XX
DT 25-FEB-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Mouse Moloney leukemia retrovirus vector LNL6 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC LHL2 from hph gene & MoMuLV
RC pLNL from N2
RC LNL-XHC from pLNL & linker
RC LNL6 from N2
RA Bender M.A., Palmer T.D., Gelinas R.E., Miller A.D.;
RT "Evidence that the packaging signal of moloney murine leukemia virus
RT extends into the gag region";
RL J. Virol. 61:1639-1646(1987).
XX
RN [2]
RP 1-300
RC LNL6 from N2
RA Kuebbing D., Friedman S., Jacobson M.;
RT ;
RL Unpublished (1989).
XX
RN [3]
RP 301-6145
RC LNL6 from N2
RA Miller A.D.;
RT ;
RL Unpublished (1989).
XX
RN [4]
RP 1-6145
RC LNL6
RA Miller A.D.;
RT ;
RL Submitted (25-FEB-1992) by:
RL Miller A.D., Program in Molecular Medicine,
RL Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
XX
RN [5]
RC N2 from MoMuLV 5' LTR & Tn5 & MoMuLV 3'LTR
RC N3 from MoMuLV 5' LTR & Tn5 & MoMuLV 3'LTR
RC pXm5 from N2 & MMLV LTR/gag & neo gene
RA Keller G., Paige P., Gilboa E., Wagner E.F.;
RT "Expression of a foreign gene in myeloid and lymphoid cells
RT derived from multipotent haematopoietic precursors";
RL Nature 318:149-154(1985).
XX
RN [6]
RC LHL from MoMULV LTR & pLG90, hph gene
RC LHL2 from MoMUSV LTR & pLG90, hph gene
RC LSHL from MoMUSV LTR & pLG90, hph gene
RC N2 from MoMULV LTR & neo gene
RC LNSAL from N2 & ADA gene
RA Palmer T.D., Hock R.A., Osborne W.R., Miller A.D.;
RT "Efficient retrovirus-mediated transfer and expression of a human
RT adenosine deaminase gene in diploid skin fibroblasts from an
RT adenosine deaminase-deficient human";
RL Proc. Natl. Acad. Sci. U.S.A. 84:1055-1059(1987).
XX
RN [7]
RC SDHT from SFFV & pFR400, dhfr gene
RC N2 from neo gene & MoMULV
RC PAM from neo gene & MoMULV
RC LNL2 from Tn5 neo gene & MoMULV
RA Miller A.D., Trauber D.R., Buttimore C.;
RT "Factors involved in the production of helper virus-free
RT retrovirus vectors";
RL Somatic Cell Mol. Genet. 12:175-183(1986).
XX
CC NCBI gi: 208850
CC NM (LNL6)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (mouse)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (N2)(MoMSV)
CC BR ()
CC OF (LN)(LNCX)(LNSX)(LXSN)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. MoMuLV BglI-XhoI, 5' LTR
FT BAL31 nuclease
FT EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT 2. Tn5 BglII-BamHI 1541bp 1516..3057, neo gene
FT EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT 3. MoMuLV BamHI-PstI, 3' LTR
FT BAL31 nuclease
FT EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT -> N2
FT 1. MoMuLV remove 1039..7673, 1698bp
FT 2. Tn5 BglII-BamHI 1541bp 1516..3057, neo gene
FT -> N2 3239bp [MoMuLV LTR]
FT 1. N2 complement ATG
FT -> LNL6 6145bp"
FT misc_recomb 144..145
FT /note="mouse DNA end/Moloney murine sarcoma virus
FT (Mo-MuSV) DNA start"
FT LTR 145..733
FT /note="Moloney murine sarcoma virus (Mo-MuSV)
FT 5' long terminal repeat"
FT misc_signal 803..1612
FT /note="extended packaging signal"
FT misc_recomb 1133..1134
FT /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT mutation 1193..1195
FT /note="GAG atg start codon to TAG stop codon"
FT misc_recomb 1616..1617
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/transposon Tn5 DNA start"
FT CDS 1656..2450
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)"
FT misc_recomb 3051..3052
FT /note="transposon Tn5 DNA end/Moloney murine
FT leukemia virus (MoMuLV) DNA start"
FT LTR 3191..3784
FT /note="Moloney murine leukemia virus (Mo-MuLV)
FT 3' long terminal repeat"
FT misc_recomb 3853..3854
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/plasmid pBR322 DNA start"
XX
SQ Sequence 6145 BP; 1397 A; 1700 C; 1607 G; 1441 T; 0 other;
gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
ggagtgggga ggcacgatgg ccgctttggt cgacccggac gggacgctcc tgcgcctgat
acagaacgaa ttgcttgcag gcatctcatg agtgtgtctt cccgttttcc gcctgaggtc
actgcgtgga tggagcgctg gcgcctgctg cgcgacggcg agctgctcac cacccactcg
agggcgtgca gcgctgcaga ggccgagtgc agaactgctc caaagggacc tcaaggcttt
ccgagggaca ctaggctgac tccatcgagc cagtgtagag ataagcttat cgattagtcc
aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
tatcacgagg ccctttcgtc ttcaa
//