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ID LORIC preliminary; circular DNA; SYN; 6604 BP.
XX
AC IG0003; ATCC37370;
XX
DT 20-NOV-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli cosmid vector Loric - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC [pPZ74 from pBR325 & pIJ303]
RC [pBRNeo from pBR322 & pRZ112, neo gene]
RC lambda NEO from lambda & pBRNeo
RC plasmid from lambda NEO
RC PiCos from pideltaplac & MUA-10
RC Loric from PiCos & plasmid
RA Little P.F., Cross S.H.;
RT "A cosmid vector that facilitates restriction enzyme mapping";
RL Proc. Natl. Acad. Sci. U.S.A. 82:3159-3163(1985).
XX
RN [2]
RC pRZ121, pRZ123, pRZ124, pRZ129 from pRZ102
RC pRZ141, pRZ143 from pRZ102
RC pRZ149, pRZ154 from pRZ143 & pRZ112
RC pRZ152 from pRZ112 & pRZ102
RC pRZ172, pRZ174 from pRZ112 & pRZ102
RC pRZ161, pRZ164, pRZ165, pRZ166 from pRZ102
RA Rothstein S.J., Jorgensen R.A., Postle K., Reznikoff W.S.;
RT "The inverted repeats of Tn5 are functionally different";
RL Cell 19:795-805(1980).
XX
RN [3]
RC piBP from piVX & human beta-globin gene
RC piBdeltaP from piBP
RC piBPSV from piBdeltaP & pPO
RC piSVHP, piSVHS from piBPSV
RC piSVHPBP from pSV0d & piBdeltaP
RC piSVHPdeltaBP from piSVHP & SV40
RC piSV from piSVHPBP
RC piSVHPplac, pSVHSplac from SV40 ori/enhancer & piSV & piVX
RC lambda HbetaG1 from Charon 4A & human beta-globin gene
RC piSVbeta from lambda HbetaG1 & piSVHPBP, human beta-globin gene
RC [pideltaplac from lacZ gene & human beta-globin gene]
RA Little P.F., Treisman R., Bierut L., Seed B., Maniatis T.;
RT "Plasmid vectors for the rapid isolation and transcriptional
RT analysis of human beta-globin gene alleles";
RL Mol. Biol. Med. 1:473-488(1983).
XX
RN [4]
RC pPO from pBR322 & SV40 ori
RA Hanahan D.;
RT ;
RL Unpublished (1983).
XX
CC ATCC size is 6270 bp.
CC Molecule is derived from the bacteriophage lambda origin of
CC replication (oriC) and may be more stable than certain cosmids.
CC Recombinants can be efficiently packaged to yield 100-300 ug
CC DNA/liter.
CC Medium is 1236 LB plus kanamycin.
CC NM (Loric)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (ATCC)
CC HO (E.coli MM294)(E.coli)
CC CP ()
CC FN (mapping site)
CC SE ()
CC PA (lambda)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 4361bp
FT transposition
FT 2. Tn5 5818bp
FT -> pRZ102 10179bp
FT 1. pRZ102 remove pBR322 sequences
FT -> pRZ112
FT 1. pRZ112 HincII-HincII 2499bp 188..2687, neo gene
FT BamHI linker 10bp nnggatccnn:BamHI linker 10bp
FT \ nnggatccnn
FT HindIII-BamHI 1493bp 1196..2689, neo/kan gene
FT 2. pBR322 remove HindIII-BamHI 346bp 30..376, 4015bp
FT -> pBR-neo 5508bp
FT 1. lambda remove HindIII-BamHI 4272bp 37460..41732,
FT \ 44230bp
FT \ HindIII 23131 25158 27480 36896 37460 44142;
FT \ BamHI 5506 22347 27973 34500 41733
FT 2. pBRNeo HindIII-BamHI 1493bp 1196..2689, neo/kan
FT -> lambda NEO 44000bp
FT 1. E. coli, lacZ gene
FT 2. human beta-globin gene
FT -> pideltaplac
FT 1. pideltaplac PstI
FT 2. MUA-10 PstI-PstI 403bp, lambda cos
FT \ lambda HindII-HindII 403bp 48299..48502..200, cos
FT -> PiCos
FT 1. PiCos EcoRI-EcoRI 630bp, lambda cos
FT Klenow:Klenow
FT 2. lambda NEO HindIII-BamHI 1493bp 1196..2689, neo/kan
FT Klenow:Klenow
FT -> Loric 6604bp"
FT misc_binding 0..0
FT /note="SIT unique BamHI-XbaI-HindIII-ClaI-SacII"
FT CDS 4631..5426
FT /note="ANT E. coli neomycin phosphotransferase II gene
FT (NPTII), neomycin resistance gene (neo)
FT kanamycin resistance gene (kan)"
FT rep_origin 6155..6557
FT /note="ORI bacteriophage lambda cos region"
XX
SQ Sequence 6604 BP; 1447 A; 1716 C; 1665 G; 1769 T; 7 other;
cgttgatttt ttaatgcaga atatgcagtt accgagatgt tccggtattt gcaaatcgaa
tggttgttgc ttccaccatg cgaggatatc ttccttctca aagtctgaca gttcagcaag
atatctgatt ccaggctttg gctttagccg cttcggttca tcagctctga tgccaatcca
cgtggtgtaa ttccctcgcc cgaaatggtc atcacagtat ttggtgaagg gaacgagttt
taatctgtca gtgcagaacg cgccgccgac gtatggagtg ccatatttct ttaccatatc
gataaatggc ttcagaacag gcattcgcgt ctgaatatcc tttggttccc ataccgtata
accatttggc tgtccaagct ccgggttgat atcaacctgc aatacggtga gcggtatatc
ccagaacttc acaacttccc tgacaaaccg atatgtcatt ggatgttcac aacctgtatc
catgaaaacg taatgcacgt ctttacctgc ccgtcgcttt tgctccatta gccagagcaa
atatgctgac gtcctgccac cggagaaact aacgacattt atcatgcagc cctgtctccc
catctcgctt tccactccag agccagtctc gcttcgtctg accacttaac gccacgctct
gtaccgaatg cctgtataag ctctaatagc tccgcaaatt cgcctacacg catcctgctg
gttgactggc ctattaccac aaagccattc ccggcaaggt taggaacaac atcctgctgc
tttaatgctg cggtaaacac acacttccag ctttctgcat ccagccagcg accatgccat
tcaacctgac gagagacgtc acctaagcag gcccatagct tcctgttttg gtctaagctg
cggttgcgtt cctgaatggt tactacgatt ggtttggttg ggtctggaag gatttgctgt
actgcgtgaa tagcgttttg ctgatgtgct ggagatcgaa tttcaaaggt tagttttttc
atgacttccc tctcccccaa ataaaaaggc ctgcgattac cagcaggcct gttattagct
cagtaatgta gatggtcatc ttttaactcc atataccgcc aatacccgtt tcatcgcggc
actctggcga cactccttaa aaaccaggtt cgtgctcatc tttccttccc gttcttccct
ggtagcaaac cggtaataca ccgttcgcca gaccttacct tcgataacca gaagacctgc
ccgtgccatt ttagccgcgg cctgatttat gctggttact gttgcgcctg ttagcgcggc
aacgtccggc gcacagaagc tattatgcgt ccccaggtaa tgaataattg cctctttgcc
cgtcatacac ttgctccttt cagtccgaac ttagctttga tttctgcgat cttcgccaga
gcctgtgcac gatttagagg tctaccgccc atgacaggaa gttgttttac tggttcaggg
atcgcctcac cacggttaat tctcgcagtc atatggacaa gctcatctgc ggccttacgg
cgtaattccg catcagtaag cgcattggcc cgcatgttct gatacaggtt ggtaaccagc
cagtagtgcg cgtttgattt ccacggataa gactccgcat ccggatacag gcctcgcttc
cggcaatact cgtaaaccat atcaaccagc tcgctgacgt ttggcagtcc ggcggtaacg
gatgcttctt cccggcacca tgcaacaaac tgcccgggtg atggcagaaa tggtcgattc
tgccgacggg ctacgcgcat tcctgcgtta acctgttcca tcgtggtgat cccgttttcc
cgaaaagcca gaacccactg gcgacggatt tcgttcactt cgttctggtc acggttagcc
aggctcgccg ggaaagttgc cagtaactgg ctgaacacac cgttgatgat ctgcgctacc
tgctgtacct gcggcttttc gtcgtactgt tccggcatgt tgttggcgat ccgacgcatc
tgctcacggt caaagttaac catctgtgcg gcgatgtttt tcatagatcc accccgtaaa
tccagtctgt gtttgtcagg tcgagttttg gtttgctggc tgtcacgcct gcctgttgct
tgttacggtt gatttcgagt tgggtccact tatcgcggag tttggccggg ctcagcacgt
taccggacca gaagttgtcc tggcatgccc agcggaacag cacacacatg tcgcggtggt
tacgtccgtc acgttcacgc atcaggcgga tatcgttagc ccacccagca aaattcggtt
ttctggctga tggtgcgata gtcttcacca tgtcaaacat ccactctgcg gcggtcaggt
cttctgctgt cccccacttg ctgccgctct gaattgcagc atccggtttc accacagaaa
ggtcgttttc tggctggtca gaggattcgc cagaattctc tgacgaataa tcttttcttt
tttcttttgt aatagtgtct tttgtgtccc cctgttttga gggatagcaa tcccccaatt
tgagggatgt tttatccctc gttttagggg attttccctc gttttgaggg atgcaccatt
ctgagatgtt tttatttggt ccaaacatgc cgccttgctg cttgataata ttcattctga
cgagttctaa cttggcttca ttgcaccgtt tgacaggtaa ctttgtaatc tcgctaagtt
gagaatcggt gattctgtcc attggtttat tccacccata ggttttacgc agaatggcaa
gcagcacttt aaactgtcgc ttggtcagat ctgcgcccga ataagcctca agcagcatat
ttgatagtct ggcgtaacca tcatcgagat ctgccacatt acgctcctgt ccggcaaagt
tacctctgcc gaagttgagt atttttgctg tatttgtcat aatgactcct gttgatagat
ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
ttttttattg gtgagaatcg cagcaacttg tcgcgccaat cgagccatgt cgtcgtcaac
gaccccccat tcaagaacag caagcagcat tgagaacttt ggaatccagt ccctcttcca
cctgctgatc tgcgacttat caacgcccac agcttccgct gtcttctcag ttccaagcat
tgcgattttg ttaagcaacg cactctcgat tcgtagagcc tcgttgcgtt tgtttgcacg
aaccatatgt aagtatttcc ttagataaca attgattgaa tgtatgcaaa taaatgcata
caccataggt gtggtttaat ttgatgccct ttttcagggc tggaatgtgt aagagcgggg
ttatttatgc tgttgttttt ttgttactcg ggaagggctt tacctcttcc gcataaacgc
ttccatcagc gtttatagtt aaaaaaatct ttcggcctgc atgaatggcc ttgttgatcg
cgctttgata tacgccgaga tctttagctg tcttggtttg cccaaagcgc attgcataat
ctttcagggt tatgcgttgt tccatacaac ctccttagta catgcaacca ttatcaccgc
cagaggtaaa atagtcaaca cgcacggtgt tagatattta tcccttgcgg tgatagattt
aacgtatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc
ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag
acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa
atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc
cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta
gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc
ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg
attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc
caagctagct tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa
cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca ctactgggct
atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
gcgccatctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc
atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
caggacgagc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
atctccagtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
tcgagcgagc acgtactcgg ctggaagccg gtcttgtcga tcaggatgat ctggacgaag
agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
gccatcacgc gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc tgcctgaggc tggacgacct
cgcggagttc taccggcagt gcaaatccgt cggcatccag gaaaccagca gcggctatcc
gcgcatccat gcccccgaac tgcaggagtg gggaggcacg atggccgctt tggtcgaccg
gacgggacgc tcctgcgcct gatacagaac gaattgcttg caggcatctc atgagtgtgt
cttcccgttt tccgcctgag gtcactgcgt ggatggagcg ctggcgcctg ctgcgcgacg
gcgagctgct caccacccac tcgagctgga tacttcgcgt ccgccagggg gacatgccgg
cgatgctgaa ggtcgcccgc attcccgatg aagaggccgg ttaccgcctg ttgacctggt
gggacgggca gggcgccccc cgactcttcg cctcggcggc gggcgctctg ctcatggagc
gcgcgtccgg ggccggggac cttgcacaga tagcgtggtc cggccaggac gacgaggctt
gcaggatcaa ttcnggatcc nnttgacatg aggttgcccc gtattcagtg tcgctgattt
gtattgtctg aagttgtttt tacgttaagt tgatgcagat caattaatac gatacctgcg
tcataattga ttatttgacg tggtttgatg gcctccacgc acgttgtgat atgtagatga
taatcattat cactttacgg gtcctttccg gtgatccgac aggttacggg gcggcgacct
cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc
ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa
agcgaggctt tttggcctct gtcgtttcct ttctctgttt ttgtccgtgg aatgaacaat
ggaagtcaac aannntctag anaagcttac tcattaggca ccccaggctt tacactttat
gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
attc
//