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ID LORIST2 preliminary; circular DNA; SYN; 5693 BP.
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AC ATCC37531;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli cosmid vector Lorist2 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC LoristE6 from LoristB & oligo, trpA terminator
RC Lorist2 from LoristE6 & oligo, rrnC terminator
RA Gibson T.J., Coulson A.R., Sulston J.E., Little P.F.;
RT "Lorist2, a cosmid with transcriptional terminators insulating
RT vector genes from interference by promoters within the insert:
RT effect on DNA yield and cloned insert frequency";
RL Gene 53:275-281(1987).
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CC Created by Moore, July 1995, under contract with NCBI.
CC Transcription terminators somewhat insulate the vector genes from
CC insert transcription.
CC Cosmid vector for genomic library construction. Insert-end-specific
CC probes may be generated from T7 and SP6 promoters.
CC Restriction digests of the clone give the following sizes (kb):
CC BamHI--5.6; HindIII--5.8. (ATCC staff)
CC Medium is 1236 LB plus kanamycin.
CC NM (Lorist2)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (ATCC)
CC HO (E.coli 1046)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (LoristE6)
CC BR ()
CC OF (Lorist6)
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. LoristE6 NheI 5646bp 1185..1185,
FT \ LoristB 1153 or may be 4104 [5517bp]
FT 2. oligo NheI-rrnC-EcoRI-NheI 47bp
FT \ ctagaaatcatccttagcgaaagctaaggattttttttatctgaatt,
FT \ rrnC terminator
FT -> Lorist2 5693bp [5564bp]"
FT - 1..1184
FT /note="LoristE6 1..1184 1184bp
FT NheI = G^CTAGC"
FT - 1185..1231
FT /note="ctagaaatcatccttagcgaaagctaaggattttttttatctgaatt
FT \ 47bp
FT NheI = G^CTAGC"
FT - 1232..5693
FT /note="LoristE6 1185..5646 4462bp"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="MCS unique HindIII-BamHI"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT terminator 200..200
FT /note="TER E. coli rrnC"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT CDS 300..1000
FT /note="GEN lambda P gene"
FT CDS 1000..2000
FT /note="GEN lambda O gene"
FT CDS 2000..2400
FT /note="GEN lambda cII gene"
FT CDS 2500..2700
FT /note="GEN lambda cro gene"
FT promoter 2700..2700
FT /note="PRO lambda pR"
FT promoter 3400..3500
FT /note="PRO E. coli neo gene"
FT CDS 3500..4300
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT); neomycin resistance gene (neo)"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda cos"
FT terminator 0..0
FT /note="TER E. coli trpA"
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SQ Sequence 5693 BP; 1300 A; 1474 C; 1455 G; 1464 T; 0 other;
atcgatccgg gcaacgttgt tgccattgct gcaggggggg gggggggggg gggggggggt
tgacttccat tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa
gcctcgcttt cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat
taagttatga cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa
aacccgcgag gtcgccgccc gtaacctgtc ggatcaccgg aaaggacccg taaagtgata
atgattatca tctacatatc acaacgtgcg tggaggccat caaaccacgt caaataatca
attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag
acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtcaaccccc cccccccccc
cccccccccc ctgcaggcgg agaactggta ggtatggaag atctctagag aattagcccg
cctaatgagc gggctttttt tgaattcgag ctcgcccggg gatcgatcct ctagagtcct
gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
cagtacaatc tgctctgatg ccgcatagtt aagccagtat atacactccg ctatcgctac
gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggcc cagctggctt atcgaaatta
atacgactca ctatagggag accggaagct taggatccta tgtattctat agtgtcacct
aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagaa atcatcctta
gcgaaagcta aggatttttt ttatctgaat tctagccaga tccccgggcg agctgcggcc
tgatttatgc tggttactgt tgcgcctgtt agcgcggcaa cgtccggcgc acagaagcta
ttatgcgtcc ccaggtaatg aataattgcc tctttgcccg tcatacactt gctcctttca
gtccgaactt agctttgatt tctgcgatct tcgccagagc ctgtgcacga tttagaggtc
taccgcccat gacaggaagt tgttttactg gttcagggat cgcctcacca cggttaattc
tcgcagtcat atggacaagc tcatctgcgg ccttacggcg taattccgca tcagtaagcg
cattggcccg catgttctga tacaggttgg taaccagcca gtagtgcgcg tttgatttcc
acggataaga ctccgcatcc ggatacaggc ctcgcttccg gcaatactcg taaaccatat
caaccagctc gctgacgttt ggcagtccgg cggtaacgga tgcttcttcc cggcaccatg
caacaaactg cccgggtgat ggcagaaatg gtcgattctg ccgacgggct acgcgcattc
ctgcgttaac ctgttccatc gtggtgatcc cgttttcccg aaaagccaga acccactggc
gacggatttc gttcacttcg ttctggtcac ggttagccag gctcgccggg aaagttgcca
gtaactggct gaacacaccg ttgatgatct gcgctacctg ctgtacctgc ggcttttcgt
cgtactgttc cggcatgttg ttggcgatcc gacgcatctg ctcacggtca aagttaacca
tctgtgcggc gatgtttttc atagatccac cccgtaaatc cagtctgtgt ttgtcaggtc
gagttttggt ttgctggctg tcacgcctgc ctgttgcttg ttacggttga tttcgagttg
ggtccactta tcgcggagtt tggccgggct cagcacgtta ccggaccaga agttgtcctg
gcatgcccag cggaacagca cacacatgtc gcggtggtta cgtccgtcac gttcacgcat
caggcggata tcgttagccc acccagcaaa attcggtttt ctggctgatg gtgcgatagt
cttcaccatg tcaaacatcc actctgcggc ggtcaggtct tctgctgtcc cccacttgct
gccgctctga attgcagcat ccggtttcac cacagaaagg tcgttttctg gctggtcaga
ggattcgcca gaattctctg acgaataatc ttttcttttt tcttttgtaa tagtgtcttt
tgtgtccccc tgttttgagg gatagcaatc ccccaatttg agggatgttt tatccctcgt
tttaggggat tttccctcgt tttgagggat gcaccattct gagatgtttt tatttggtcc
aaacatgccg ccttgctgct tgataatatt cattctgacg agttctaact tggcttcatt
gcaccgtttg acaggtaact ttgtaatctc gctaagttga gaatcggtga ttctgtccat
tggtttattc cacccatagg ttttacgcag aatggcaagc agcactttaa actgtcgctt
ggtcagatct gcgcccgaat aagcctcaag cagcatattt gatagtctgg cgtaaccatc
atcgagatct gccacattac gctcctgtcc ggcaaagtta cctctgccga agttgagtat
ttttgctgta tttgtcataa tgactcctgt tgatagatcc agtaatgacc tcagaactcc
atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatcgca
gcaacttgtc gcgccaatcg agccatgtcg tcgtcaacga ccccccattc aagaacagca
agcagcattg agaactttgg aatccagtcc ctcttccacc tgctgatctg cgacttatca
acgcccacag cttccgctgt cttctcagtt ccaagcattg cgattttgtt aagcaacgca
ctctcgattc gtagagcctc gttgcgtttg tttgcacgaa ccatatgtaa gtatttcctt
agataacaat tgattgaatg tatgcaaata aatgcataca ccataggtgt ggtttaattt
gatgcccttt ttcagggctg gaatgtgtaa gagcggggtt atttatgctg ttgttttttt
gttactcggg aagggcttta cctcttccgc ataaacgctt ccatcagcgt ttatagttaa
aaaaatcttt cggcctgcat gaatggcctt gttgatcgcg ctttgatata cgccgagatc
tttagctgtc ttggtttgcc caaagcgcat tgcataatct ttcagggtta tgcgttgttc
catacaacct ccttagtaca tgcaaccatt atcaccgcca gaggtaaaat agtcaacacg
cacggtgtta gatatttatc ccttgcggtg atagatttaa cgtatgagca caaaaaagaa
accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa
gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc
aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct
tacaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta
cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt
tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga
tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt
tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctagcttc acgctgccgc
aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag
aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
gactgtggcc ggctgggtgt ggcgaccgct atcaggacat agcgttggct acccgtgata
ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccgggc tcgatcccct
cgcgagttgg ttcagctgct gcctgaggct ggacgacctc gcggagttct accggcagtg
caaatccgtc ggcatccagg aaaccagcag cggctatccg cgcatccatg cccccgaact
gcaggagtgg ggaggcacga tggccgcttt ggtcggatca attcgcgcga ccg
//