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ID LORISTB preliminary; circular DNA; SYN; 5614 BP.
XX
AC M15423;
XX
DT 16-JUL-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli cosmid vector LoristB - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-5614
RC pUCSP from pUC12 & pSP64, Sp6 promoter
RC pUCT7 from pUC12 & pT7-2, T7 promoter
RC Lorus from Loric & pUCSP
RC Lordel from Lorus
RC LordelO from Lordel
RC LordelB from LordelO
RC LdBp12 from LordelB & pUC12
RC Loris from LdBp12
RC pUCT7Bdelta from pUCT7
RC LorspT7 from Loris & pUCT7Bdelta
RC LoristB from LorspT7
RA Cross S.H., Little P.F.;
RT "A cosmid vector for systematic chromosome walking";
RL Gene 49:9-22(1986).
XX
CC NM (LoristB)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (chromosome walking)
CC SE ()
CC PA (lambda)(Sp6)(T7)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC12 remove BamHI-HindIII 27bp 249..276,
FT \ MCS/2653bp
FT 2. pSP64 HindIII-Sau3AI/MboI 256bp 2751..2999..8,
FT \ Sp6 promoter
FT -> pUCSP 2909bp
FT 1. Loric ClaI-HindIII 6192bp 300..6492
FT :Klenow
FT 2. pUCSP HindIII-EcoRI 274bp 231..249 - 276,
FT \ Sp6 promoter
FT :Klenow
FT -> Lorus 6466bp
FT 1. Lorus remove SspI-SstII/SacII 1411bp,
FT \ Loric 1279..2690/5193bp
FT T4 DNA polymerase
FT -> Lordel 5193bp
FT 1. Lordel BamHI 5193bp, Loric 6076..6076
FT Klenow
FT -> LordelO 5193bp [ClaI]
FT 1. LordelO HindIII-HindIII 5181bp,
FT \ Loric 6504..6604..6492
FT Klenow
FT EcoRI 5181bp, Loric 2494..2494
FT BamHI linker 6bp ggatcc
FT -> LordelB 5187bp
FT 1. LordelB XbaI 5187bp, Loric 6497..6497
FT Klenow
FT 2. pUC12 EcoRI 2680bp 231..231
FT Klenow
FT -> LdBp12 7867bp
FT 1. LdBp12 remove HindIII-HindIII, pUC12 ori
FT \ pUC12 276..276
FT -> Loris 7800bp [SstI]
FT 1. pUC12 remove HindIII-HaeII 168bp 276..444,
FT \ MCS/2512bp
FT 2. pT7-2 HindIII-HaeII 202bp 239..441, T7 promoter
FT -> pUCT7 2714bp
FT 1. pUCT7 BamHI 2714bp 249..249
FT Klenow
FT -> pUCT7Bdelta 2714bp
FT 1. Loris HindIII 7800bp, pUC12 276..276
FT 2. pUCT7Bdelta HindIII 2714bp 276..276
FT -> LorspT7 10500bp
FT 1. LorspT7 remove SstI/SacI-SstI/SacI,
FT \ pUC12 ori/pUC12 241
FT -> LoristB 5614bp"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cos region"
XX
SQ Sequence 5614 BP; 1279 A; 1460 C; 1440 G; 1435 T; 0 other;
atcgatccgg gcaacgttgt tgccattgct gcaggggggg gggggggggg gggggggggt
tgacttccat tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa
gcctcgcttt cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat
taagttatga cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa
aacccgcgag gtcgccgccc gtaacctgtc ggatcaccgg aaaggacccg taaagtgata
atgattatca tctacatatc acaacgtgcg tggaggccat caaaccacgt caaataatca
attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag
acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtcaaccccc cccccccccc
cccccccccc ctgcaggcgg agaactggta ggtatggaag atctctagag aattcgagct
cgcccgggga tcgatcctct agagtcctga tgcggtattt tctccttacg catctgtgcg
gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
gccagtatat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
gcgaggccca gctggcttat cgaaattaat acgactcact atagggagac cggaagctta
ggatcctatg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
gcagggtcat cgctagccag atccccgggc gagctgcggc ctgatttatg ctggttactg
ttgcgcctgt tagcgcggca acgtccggcg cacagaagct attatgcgtc cccaggtaat
gaataattgc ctctttgccc gtcatacact tgctcctttc agtccgaact tagctttgat
ttctgcgatc ttcgccagag cctgtgcacg atttagaggt ctaccgccca tgacaggaag
ttgttttact ggttcaggga tcgcctcacc acggttaatt ctcgcagtca tatggacaag
ctcatctgcg gccttacggc gtaattccgc atcagtaagc gcattggccc gcatgttctg
atacaggttg gtaaccagcc agtagtgcgc gtttgatttc cacggataag actccgcatc
cggatacagg cctcgcttcc ggcaatactc gtaaaccata tcaaccagct cgctgacgtt
tggcagtccg gcggtaacgg atgcttcttc ccggcaccat gcaacaaact gcccgggtga
tggcagaaat ggtcgattct gccgacgggc tacgcgcatt cctgcgttaa cctgttccat
cgtggtgatc ccgttttccc gaaaagccag aacccactgg cgacggattt cgttcacttc
gttctggtca cggttagcca ggctcgccgg gaaagttgcc agtaactggc tgaacacacc
gttgatgatc tgcgctacct gctgtacctg cggcttttcg tcgtactgtt ccggcatgtt
gttggcgatc cgacgcatct gctcacggtc aaagttaacc atctgtgcgg cgatgttttt
catagatcca ccccgtaaat ccagtctgtg tttgtcaggt cgagttttgg tttgctggct
gtcacgcctg cctgttgctt gttacggttg atttcgagtt gggtccactt atcgcggagt
ttggccgggc tcagcacgtt accggaccag aagttgtcct ggcatgccca gcggaacagc
acacacatgt cgcggtggtt acgtccgtca cgttcacgca tcaggcggat atcgttagcc
cacccagcaa aattcggttt tctggctgat ggtgcgatag tcttcaccat gtcaaacatc
cactctgcgg cggtcaggtc ttctgctgtc ccccacttgc tgccgctctg aattgcagca
tccggtttca ccacagaaag gtcgttttct ggctggtcag aggattcgcc agaattctct
gacgaataat cttttctttt ttcttttgta atagtgtctt ttgtgtcccc ctgttttgag
ggatagcaat cccccaattt gagggatgtt ttatccctcg ttttagggga ttttccctcg
ttttgaggga tgcaccattc tgagatgttt ttatttggtc caaacatgcc gccttgctgc
ttgataatat tcattctgac gagttctaac ttggcttcat tgcaccgttt gacaggtaac
tttgtaatct cgctaagttg agaatcggtg attctgtcca ttggtttatt ccacccatag
gttttacgca gaatggcaag cagcacttta aactgtcgct tggtcagatc tgcgcccgaa
taagcctcaa gcagcatatt tgatagtctg gcgtaaccat catcgagatc tgccacatta
cgctcctgtc cggcaaagtt acctctgccg aagttgagta tttttgctgt atttgtcata
atgactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
gctcggttgc cgccgggcgt tttttattgg tgagaatcgc agcaacttgt cgcgccaatc
gagccatgtc gtcgtcaacg accccccatt caagaacagc aagcagcatt gagaactttg
gaatccagtc cctcttccac ctgctgatct gcgacttatc aacgcccaca gcttccgctg
tcttctcagt tccaagcatt gcgattttgt taagcaacgc actctcgatt cgtagagcct
cgttgcgttt gtttgcacga accatatgta agtatttcct tagataacaa ttgattgaat
gtatgcaaat aaatgcatac accataggtg tggtttaatt tgatgccctt tttcagggct
ggaatgtgta agagcggggt tatttatgct gttgtttttt tgttactcgg gaagggcttt
acctcttccg cataaacgct tccatcagcg tttatagtta aaaaaatctt tcggcctgca
tgaatggcct tgttgatcgc gctttgatat acgccgagat ctttagctgt cttggtttgc
ccaaagcgca ttgcataatc tttcagggtt atgcgttgtt ccatacaacc tccttagtac
atgcaaccat tatcaccgcc agaggtaaaa tagtcaacac gcacggtgtt agatatttat
cccttgcggt gatagattta acgtatgagc acaaaaaaga aaccattaac acaagagcag
cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta
tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt
aatggcatca atgcattaaa tgcttataac gccgcattgc ttacaaaaat tctcaaagtt
agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt
agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca
gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc
acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc
gcaccaacag gctccaagcc aagctagctt cacgctgccg caagcactca gggcgcaagg
gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
tggcgaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
ctcatgctgg agttcttcgc ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc
tgcctgaggc tggacgacct cgcggagttc taccggcagt gcaaatccgt cggcatccag
gaaaccagca gcggctatcc gcgcatccat gcccccgaac tgcaggagtg gggaggcacg
atggccgctt tggtcggatc aattcgcgcg accg
//