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ID LXSN preliminary; circular DNA; SYN; 5874 BP.
XX
AC M28248;
XX
DT 28-JAN-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Mouse Moloney murine sarcoma virus vector LXSN - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-5874
RC LN, LNSX, LNCX, LXSN from LNL6
RA Miller A.D.;
RT ;
RL Unpublished (1989).
XX
RN [2]
RC LN, LNSX, LNCX, LXSN from LNL6
RA Miller A.D., Rosman G.J.;
RT "Improved retroviral vectors for gene transfer and expression";
RL Biotechniques 7:980-990(1989).
XX
RN [3]
RC LXSN
RA Miller A.D.;
RT ;
RL Submitted (22-SEP-1989) to GenBank on computer-readable media by:
RL Miller A.D., Program in Molecular Medicine,
RL Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
XX
RN [4]
RC LN, LNSX, LNCX, LXSN from LNL6
RA Kuebbing D., Freidman S.;
RT ;
RL Unpublished (1991).
XX
CC retrovirus
CC NM (LXSN)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (mouse)
CC CP ()
CC FN (gene transfer)(expression)
CC SE ()
CC PA (LNL6)
CC BR (LN)(LNCX)(LNSX)
CC OF ()
CC OR (junction of neomycin and mouse genomic sequences)
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. MMLV, retroviral LTR/packaging signal
FT 2. linker EcoRI-HpaI-XhoI-BamHI 24bp
FT \ gaattcgttaacctcgagggatcc
FT 3. SV40 661bp, early promoter
FT 4. Tn5, neo gene
FT 5. MMLV, retroviral LTR/polyA
FT -> LXSN 5874bp"
FT misc_recomb 174..175
FT /note="mouse DNA end/Moloney murine sarcoma virus
FT (Mo-MuSV) DNA start"
FT LTR 175..763
FT /note="RPT Moloney murine sarcoma virus (Mo-MuSV)
FT 5' long terminal repeat"
FT misc_signal 833..1642
FT /note="extended packaging signal"
FT misc_recomb 1163..1164
FT /note="Moloney murine sarcoma virus (Mo-MuSV) DNA
FT end/Moloney murine leukemia virus (Mo-MuLV) DNA start"
FT mutation 1223..1225
FT /note="GAG atg start codon to TAG stop codon"
FT misc_recomb 1654..1655
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/SV40 (SV40) DNA start"
FT promoter 1655..2020
FT /note="PRO SV40 early gene"
FT misc_recomb 2020..2021
FT /note="SV40 DNA end/transposon Tn5
FT DNA start"
FT CDS 2066..2860
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)"
FT misc_recomb 2877..2878
FT /note="transposon Tn5 DNA end/Moloney murine
FT leukemia virus (Mo-MuLV) DNA start"
FT LTR 2920..3513
FT /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT 3' long terminal repeat"
FT misc_recomb 3582..3583
FT /note="Moloney murine leukemia virus (Mo-MuLV) DNA
FT end/plasmid pBR322 DNA start"
XX
SQ Sequence 5874 BP; 1351 A; 1611 C; 1515 G; 1397 T; 0 other;
gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt
gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
tttttggagg cctaggcttt tgcaaaaagc ttgggctgca ggtcgaggcg gatctgatca
agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgat
aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc
cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg
tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa
//