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ID M13BM20 preliminary; circular DNA; SYN; 7286 BP.
XX
AC IG0305;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector M13BM20 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-7249
RC M13mp18 from M13mp8
RC M13mp19 from M13mp9
RC pUC18 from pUC8
RC pUC19 from pUC9
RA Yanisch-Perron C., Vieira J., Messing J.;
RT "Improved M13 phage cloning vectors and host strains: nucleotide
RT sequences of the M13mp18 and pUC19 vectors";
RL Gene 33:103-119(1985).
XX
CC M13 is not lytic; the phage extrude through the cell wall.
CC The replicative RF I form in the cell is double-stranded and circular,
CC and acts like a plasmid.
CC The phage form outside the cell is linear and single-stranded.
CC NM (M13BM20)
CC CM (yes)
CC NA (filamentous ss-DNA)(ds-DNA)
CC TP (linear)(circular)
CC ST ()
CC TY (phage)
CC SP (Boehringer)
CC HO (E.coli JM105)(E.coli JM109)
CC CP ()
CC FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC FN (labeled strand-specific DNA)
CC SE (color blue/white)
CC PA (M13mp18)
CC BR (M13BM21)
CC OF ()
CC OR (E.coli JM103)
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. M13mp18 remove EcoRI-HindIII 51bp
FT \ 6231..6282, MCS/7198bp
FT 2. oligo EcoRI-HindIII 88bp, MCS
FT -> M13BM20 7286bp"
FT CDS join(6850..7181,1..831)
FT /note="GEN bacteriophage M13 gene II (rf replication
FT and nicking)"
FT CDS 496..831
FT /note="GEN bacteriophage M13 gene X"
FT CDS 843..1106
FT /note="GEN bacteriophage M13 gene V (single stranded
FT binding protein)"
FT CDS 1108..1209
FT /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT CDS 1206..1304
FT /note="GEN bacteriophage M13 gene IX (minor coat
FT protein)"
FT misc_binding 1270..1270
FT /note="SIT unique SnaBI"
FT CDS 1301..1522
FT /note="GEN bacteriophage M13 gene VIII (major coat
FT protein)"
FT CDS 1579..2853
FT /note="GEN bacteriophage M13 gene III (adsorption
FT protein)"
FT misc_binding 1752..1752
FT /note="SIT unique BsmI"
FT CDS 2856..3194
FT /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT CDS 3196..4242
FT /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT CDS 4220..5500
FT /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage M13"
FT misc_binding 5615..5615
FT /note="SIT unique NaeI"
FT misc_binding 5721..5721
FT /note="SIT unique DraIII"
FT rep_origin >5615..<5914
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT from pBR322 bases 2354..2373"
FT misc_binding 5914..5914
FT /note="SIT unique AvaII"
FT misc_binding 6002..6002
FT /note="SIT unique NarI"
FT promoter 0..0
FT /note="PRO E. coli lac operator region"
FT CDS <6228..<6935
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 forward -40"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 universal"
FT misc_binding 6228..6293
FT /note="MCS EcoRI-SmaI-BamHI-MluI-BfrI-NotI-EclXI-
FT KspI-KpnI-ApaI-SalI-XbaI-SacI-PstI-SphI-EcoRV-NcoI-
FT HindIII"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 reverse"
FT misc_binding 6408..6408
FT /note="SIT unique PvuI"
FT misc_binding 6437..6437
FT /note="SIT unique BglI"
FT misc_binding 6501..6501
FT /note="SIT unique SauI"
FT misc_binding 0..0
FT /note="SIT unique MstII"
FT misc_binding 6935..6935
FT /note="SIT unique BglII"
XX
SQ Sequence 7286 BP; 1773 A; 1551 C; 1545 G; 2417 T; 0 other;
aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcccgg
ggatccacgc gtcttaaggc ggccgcggta ccgggcccgt cgactctaga gctctgcagg
catgcgatat cccatggaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
cttcctgagg ccgatacggt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
cagacgcgaa ttatttttga tggcgttcct attggttaaa aaatgagctg atttaacaaa
aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaatattt gcttatacaa
tcttcctgtt tttggggctt ttctgattat caaccggggt acatatgatt gacatgctag
ttttacgatt accgttcatc gattctcttg tttgctccag actctcaggc aatgacctga
tagcctttgt agatctctca aaaatagcta ccctctccgg cattaattta tcagctagaa
cggttgaata tcatattgat ggtgatttga ctgtctccgg cctttctcac ccttttgaat
ctttacctac acattactca ggcattgcat ttaaaatata tgagggttct aaaaattttt
atccttgcgt tgaaataaag gcttctcccg caaaagtatt acagggtcat aatgtttttg
gtacaaccga tttagcttta tgctctgagg ctttattgct taattttgct aattctttgc
cttgcctgta tgatttattg gatgtt
//