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ID M13MC18 preliminary; circular DNA; SYN; 2514 BP.
XX
AC IG5086;
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DT 01-OCT-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phage vector M13mc18 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC lambda SK4 from EMBL3 & oligo
RC lambda SK6 from lambda 2001 & oligo
RC lambda SK10 from lambda SK9 & oligo
RC lambda SK20 from lambda SK10 & oligo
RC lambda SK21 from lambda SK9 & lambda GEM11
RC lambda SK22 from lambda SK9 & oligo
RC lambda SR9-B from lambda SK18 & oligo
RC lambda SK23 from lambda SK10 & EMBL3 & lambda SR9-B
RC lambda SK24 from lambda SK22 & EMBL3 & lambda SR9-B
RC lambda SK25 from lambda SK22 & oligo
RC lambda SK27 from lambda SK22
RC lambda SK40 from lambda SK10 & oligo
RC lambda SK41 from lambda SK10 & oligo
RC lambda SK16 from lambda SK15 & oligo
RC lambda SK17 from lambda SK15 & oligo
RC lambda SK28 from lambda SK17
RA Zabarovsky E.R., Klein G., Winberg G.;
RT "Lambda SK diphasmids: phage lambda vectors for genomic, jumping,
RT linking and cDNA libraries";
RL Gene 127:1-14(1993).
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RN [2]
RC placZ from pBR322 & Charon 4A, lacZ gene
RC phage EL3/4 from EMBL3 & EMBL4 & placZ
RC lambda SK3 from EMBL3 & phage EL3/4
RC phage SK-P from EMBL4 & linker
RC lambda SK5 from lambda SK3 & phage SK-P
RC lambda SK1, lambda SK2, lambda SK6 from pHC79 & M13mp18
RC lambda SK18, lambda SK18' from pHC79 & M13mp18
RC lambda SK2A from lambda SK18
RC lambda SK2deltaSR from lambda SK2
RC lambda SK9 from EMBL3 & lambda SK2deltaSR
RC lambda SK10 from lambda SK9 & oligo
RC lambda SK11 from lambda gt11 & oligo
RC lambda SK12 from lambda SK2A & phage EL3/4
RC lambda SK15 from lambda SK9 & pUC4K
RC lambda SK16 from lambda SK15 & oligo
RC lambda SK17 from lambda SK15 & oligo
RC lambda SK20 from lambda SK10 & oligo
RC lambda SK21 from lambda SK9 & pGEM-11Zf+
RC lambda SK22 from lambda SK9 & oligo
RC pSR9 from lambda SK2
RC lambda SK23 from lambda SK10 & oligo & pSR9
RC lambda SK24 from lambda SK22 & lambda SK23
RC M13mc18 from M13mp18 & pHC79
RC M13mc19 from M13mp19 & pHC79
RA Zabarovsky E.R., Winberg G.;
RT "Improved methods and new vectors for creating the clone libraries
RT of genes";
RL Sov. Sci. Rev. D. Physiochem. Biol. 11:1-65(1992).
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RN [3]
RC lambda pMYF15 from piAN7 & lambda L47.1AB
RC lambda pMYF17 from lambda pMYF15 & lambda gtWES.lambdaC
RC lambda pMYF131 from lambda pMYF17 & pUC19
RC lambda pSL5 from lambda pMYF131 & lambda L47.1
RC lambda pMK2 from lambda pMYF131 & ptl gene
RA Yankovsky N.K., Fonstein M.Y., Laskina S.Y., Bukanov N.O.,
RA Yakubovich N.V., Ermakova L.M., Rebentish B.A., Janulaitis A.A.,
RA Debabov V.G.;
RT "Phasmids as effective and simple tools for construction and
RT analysis of gene libraries";
RL Gene 81:203-210(1989).
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RN [4]
RC SK18 from lambda
RA Zabarovsky E.R., Winberg G., Boldog F., Turina O.T., Nurbekov M.K.,
RA Marcsek Z., Erlandsson R., Grigorieva A.Y., Stanbridge E.J.,
RA Sumegi J., Kisselev L.L., Klein G.;
RT "New generation of vectors for cloning and mapping of the human
RT genome";
RL Adv. Mol. Gen. 4:281-293(1991).
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RN [5]
RC [lambda 47.1c+ from lambda]
RC [lambda L47.1, lambda L47.1AB from lambda]
RC lambda pMYF11 from pBR322 & lambda 47.1c+
RA Yankovsky N.K., Fonstein M.Y., Debabov V.G.;
RT "Monomer circular phasmid DNA molecules can be effectively packaged
RT in vitro and used as vector DNA molecules";
RL Biotech 85 Europe 85:709-714(1985).
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CC Created by Moore, July 1995, under contract with NCBI.
CC MCS. oligonucleotide linker.
CC NM (M13mc18)
CC CM (yes)
CC NA (filamentous ss-DNA)(ds-DNA)
CC TP (linear)(circular)
CC ST ()
CC TY (phage)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC FN (labeled strand-specific DNA)
CC SE ()
CC PA (M13mp18)(pHC79)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pHC79 EcoRI-SalI 653bp 6523..6524..652,
FT \ lambda cos
FT Klenow:Klenow
FT 2. M13mp18 BalI-BglII 1853bp 5082..6935,
FT \ M13 ori/lacI/lacZ/MCS
FT Klenow:Klenow
FT -> M13mc18 2506bp"
FT - 1..657
FT /note="pHC79 6523..6524..655 653bp
FT SalI = G^TCGA C
FT BalI = TGG^CCA"
FT - 658..2514
FT /note="M13mp18 5082..6938 1857bp
FT BglII = A^GATC T
FT EcoRI = G^AATTC"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 2514 BP; 542 A; 683 C; 618 G; 671 T; 0 other;
aattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta
aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta
tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc
cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg
accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgacca
gaatgtccct tttattactg gtcgtgtgac tggtgaatct gccaatgtaa ataatccatt
tcagacgatt gagcgtcaaa atgtaggtat ttccatgagc gtttttcctg ttgcaatggc
tggcggtaat attgttctgg atattaccag caaggccgat agtttgagtt cttctactca
ggcaagtgat gttattacta atcaaagaag tattgctaca acggttaatt tgcgtgatgg
acagactctt ttactcggtg gcctcactga ttataaaaac acttctcaag attctggcgt
accgttcctg tctaaaatcc ctttaatcgg cctcctgttt agctcccgct ctgattccaa
cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc tgtagcggcg
cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
cggaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cgctggtgaa
aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc
ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatacggtc
gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
ggcgttccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt
tctgattatc aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg
attctcttgt ttgctccaga ctctcaggca atgacctgat agcctttgta gatc
//