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ID M13MP10 preliminary; circular DNA; SYN; 7244 BP.
XX
AC L08819; VB0016; IG0004; IG0005; M23239;
XX
DT 20-NOV-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector M13mp10 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-7244
RC M13mp10
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
RN [2]
RC M13mp8 from M13mp7 & pUC8
RC M13mp9 from M13mp7 & pUC9
RC M13mp10 from M13mp8 & linker
RC M13mp11 from M13mp9 & linker
RA Messing J., Vieira J.;
RT "A new pair of M13 vectors for selecting either DNA strand of
RT double-digest restriction fragments";
RL Gene 19:269-276(1982).
XX
CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Assembled from M13mp18
CC by W. Gilbert, Whitaker College, MIT and
CC by F. Pfeiffer, MPI, Martinsried
CC
CC M13 is not lytic; the phage extrude through the cell wall.
CC The replicative RF I form in the cell is double-stranded and
CC circular, and acts like a plasmid.
CC The phage form outside the cell is linear and single-stranded.
CC Amersham sequence is 7174 bp.
CC NM (M13mp10)
CC CM (yes)
CC NA (filamentous ss-DNA)(ds-DNA)
CC TP (linear)(circular)
CC ST ()
CC TY (phage)
CC SP (Amersham)
CC HO (E.coli JM101)(E.coli JM103)(E.coli JM105)
CC HO (E.coli JM107)(E.coli JM109)(E.coli DH5alphaF')
CC CP ()
CC FN (cloning)(sequencing dideoxy)(in vitro mutagenesis)
CC FN (labeled strand-specific DNA)
CC SE (color blue/white)
CC PA (M13mp7)(M13mp8)
CC BR (M13mp11)
CC OF (pUC12)(M13mp18)(M13mIC7)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. M13mp8 7229bp
FT 2. SstI/SacI-XbaI linker 12bp gagctctctaga
FT -> M13mp10 7244bp"
FT CDS join(6843..7174,1..831)
FT /note="GEN bacteriophage M13 gene II (rf replication
FT and nicking)"
FT CDS 496..831
FT /note="GEN bacteriophage M13 gene X"
FT CDS 843..1106
FT /note="GEN bacteriophage M13 gene V (single stranded
FT binding protein)"
FT CDS 1108..1209
FT /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT CDS 1206..1304
FT /note="GEN bacteriophage M13 gene IX (minor coat
FT protein)"
FT CDS 1301..1522
FT /note="GEN bacteriophage M13 gene VIII (major coat
FT protein)"
FT CDS 1579..2853
FT /note="GEN bacteriophage M13 gene III (adsorption
FT protein)"
FT CDS 2856..3194
FT /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT CDS 3196..4242
FT /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT CDS 4220..5500
FT /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT rep_origin complement(5488..5868)
FT /note="ORI bacteriophage M13 intergenic region
FT (M13IG)"
FT promoter 6095..6216
FT /note="PRO E. coli lac operator region"
FT CDS 6217..6705
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT misc_feature 5869..6230
FT /note="E. coli lac operon"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 -40"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 universal"
FT misc_feature 6231..6281
FT /note="MCS EcoRI-SacI-SmaI-BamHI-XbaI-SalI-PstI-
FT HindIII
FT pUC12 polylinker"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 reverse"
FT misc_feature 6284..6705
FT /note="E. coli lac operon"
FT misc_feature 6706..7244
FT /note="from phage M13"
XX
SQ Sequence 7244 BP; 1766 A; 1539 C; 1530 G; 2409 T; 0 other;
aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgagc
tcgcccgggg atcctctaga gtcgacctgc agcccaagct tggcactggc cgtcgtttta
caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
agctggctgg agtgcgatct tcctgaggcc gatacggtcg tcgtcccctc aaactggcag
atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg
ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttcctat tggttaaaaa
atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca accggggtac
atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt tgctccagac
tctcaggcaa tgacctgata gcctttgtag atctctcaaa aatagctacc ctctccggca
ttaatttatc agctagaacg gttgaatatc atattgatgg tgatttgact gtctccggcc
tttctcaccc ttttgaatct ttacctacac attactcagg cattgcattt aaaatatatg
agggttctaa aaatttttat ccttgcgttg aaataaaggc ttctcccgca aaagtattac
agggtcataa tgtttttggt acaaccgatt tagctttatg ctctgaggct ttattgctta
attttgctaa ttctttgcct tgcctgtatg atttattgga tgtt
//