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ID M13MP18 preliminary; circular DNA; SYN; 7249 BP.
XX
AC X02513; M77815; M11454; VB0018;
XX
DT 02-JUL-1986 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector M13mp18 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-7249
RC M13mp18 from M13mp8
RC M13mp19 from M13mp9
RC pUC18 from pUC8
RC pUC19 from pUC9
RA Yanisch-Perron C., Vieira J., Messing J.;
RT "Improved M13 phage cloning vectors and host strains: nucleotide
RT sequences of the M13mp18 and pUC19 vectors";
RL Gene 33:103-119(1985).
XX
RN [2]
RC M13mp1 from M13 & pMG1106
RC M13mp2 from M13mp1
RC M13mp5 from M13mp2
RC M13mp6 from M13mp2
RC [M13am1, M13am2 from M13]
RC M13mp61 from M13mp6 & M13am1 & M13am2
RC M13mp62 from M13mp61
RC M13mp63 from M13mp62
RC mWU43 from M13mp2
RC mWJ22 from M13mp2
RC M13mp71 from M13mWJ43
RC M13mp7 from M13mp71 & M13mp63
RC M13mp8 from M13mp7 & pUC8
RC M13mp9 from M13mp7 & pUC9
RC [M13mp8c from M13mp8]
RC M13mp10 from M13mp8c & pUC12
RC M13mp11 from M13mp8c & pUC13
RC M13Ho176 from M13 & lambda h80dhis
RC M13Gori1 from M13 & phage G4
RC M13bla from M13 & Tn3
RC M13blacat from M13 & Tn3 & pACYC184
RC R199 from phage f1
RC R208 from R199
RC R209 from phage f1
RC R229 from phage f1
RC fd11 from phage fd & pKB252
RC fd101 from phage fd & pACYC177
RC fd103 from phage fd & pACYC177 & pACYC184
RC fd104 from phage fd & pACYC177
RC fd106 from phage fd & pACYC177 & pACYC184
RC fd109 from phage fd & pBR322
RC fd107 from phage fd & pBR322
RC fd-tet from phage fd & Tn10
RC fKN16 from fd-tet
RA Messing J.;
RT "New M13 Vectors for Cloning";
RL Meth. Enzymol. 101:20-78(1983).
XX
RN [3]
RC from M13 series
RA Sanger F., Nicklen S., Coulson A.R.;
RT "DNA sequencing with chain-terminating inhibitors";
RL Proc. Natl. Acad. Sci. U.S.A. 74:5463-5468(1977).
XX
RN [4]
RC from M13mp5
RC from M13mp7
RC from M13mp8
RC from M13mp9
RA Zoller M.J., Smith M.;
RT "Oligonucleotide-directed mutagenesis of DNA fragments cloned into
RT M13 vectors";
RL Meth. Enzymol. 100:468-500(1983).
XX
RN [5]
RC pHM235 from pBR325 & primer
RA Hu N.T., Messing J.;
RT "The making of strand-specific M13 probes";
RL Gene 17:271-277(1982).
XX
RN [6]
RC [M13mp2963 from M13mp2]
RC pHM232 from pBR325 & M13mp2963
RA Heidecker G., Messing J., Gronenborn B.;
RT "A versatile primer for DNA sequencing in the M13mp2 cloning
RT system";
RL Gene 10:69-73(1980).
XX
RN [7]
RC from M13mp18, M13 gene III
RC from M13mp19, M13 gene III
RA Ebright R., Dong Q., Messing J.;
RT "Corrected nucleotide sequence of M13mp18 gene III";
RL Gene 114:81-83(1992).
XX
RN [8]
RC M13mp18 from M13mp10
RC M13mp19 from M13mp11
RA Norrander J., Kempe T., Messing J.;
RT "Construction of improved M13 vectors using
RT oligodeoxynucleotide-directed mutagenesis";
RL Gene 26:101-106(1983).
XX
RN [9]
RC from M13mp18
RC from M13mp19
RA Hong G.F.;
RT "A method for sequencing single-stranded cloned DNA in both
RT directions";
RL Biosci. Rep. 1:243-252(1981).
XX
RN [10]
RC from pBR322, primers
RC from M13mp2, primers
RC from M13mp7, primers
RC from M13bla6, primers
RA Wallace R.B., Johnson M.J., Suggs S.V., Miyoshi K., Bhatt R.,
RA Itakura K.;
RT "A set of synthetic oligodeoxyribonucleotide primers for DNA
RT sequencing in the plasmid vector pBR322";
RL Gene 16:21-26(1981).
XX
RN [11]
RC M13mp5 from M13mp2
RC M13mp6 from M13mp2
RA Messing J.;
RT "Multipurpose cloning system based on the single-stranded DNA
RT bacteriophage M13";
RL Recombinant DNA Technical Bulletin NIH 2:43-48(1979).
XX
RN [12]
RP 1-7250
RC M13mp18 from M13mp8
RC M13mp19 from M13mp9
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
CC Size is 7250 for Boehringer.
CC M13 is not lytic; the phage extrude through the cell wall.
CC The replicative RF I form in the cell is double-stranded and circular,
CC and acts like a plasmid.
CC The phage form outside the cell is linear and single-stranded.
CC NCBI gi: 58253
CC NM (M13mp18)
CC CM (yes)
CC NA (filamentous ss-DNA)(ds-DNA)
CC TP (linear)(circular)
CC ST ()
CC TY (phage)
CC SP (Boehringer)(Bio-Rad)(BRL)(NEB)(Pharmacia)(Sigma)(USB)
CC HO (E.coli JM101)(E.coli JM103)(E.coli JM105)(E.coli CJ236)
CC HO (E.coli JM107)(E.coli JM109)(E.coli DH5alphaF')(E.coli NM522)
CC CP ()
CC FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC FN (labeled strand-specific DNA)
CC SE (color blue/white)
CC PA (M13mp8)
CC BR (M13mp19)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. M13mp10 7244bp, MCS
FT 2. oligo SphI-KpnI 12bp gcatgcggtacc
FT -> M13mp18 7249bp"
FT CDS join(6850..7181,1..831)
FT /note="GEN bacteriophage M13 gene II (rf replication
FT and nicking)"
FT CDS 496..831
FT /note="GEN bacteriophage M13 gene X"
FT CDS 843..1106
FT /note="GEN bacteriophage M13 gene V (single stranded
FT binding protein)"
FT CDS 1108..1209
FT /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT CDS 1206..1304
FT /note="GEN bacteriophage M13 gene IX (minor coat
FT protein)"
FT misc_binding 1270..1270
FT /note="SIT unique SnaBI"
FT CDS 1301..1522
FT /note="GEN bacteriophage M13 gene VIII (major coat
FT protein)"
FT CDS 1579..2853
FT /note="GEN bacteriophage M13 gene III (adsorption
FT protein)"
FT misc_binding 1752..1752
FT /note="SIT unique BsmI"
FT CDS 2856..3194
FT /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT CDS 3196..4242
FT /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT CDS 4220..5500
FT /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage M13 intergenic region
FT (M13IG)"
FT misc_binding 5615..5615
FT /note="SIT unique NaeI"
FT misc_binding 5721..5721
FT /note="SIT unique DraIII"
FT rep_origin >5615..<5914
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT from pBR322 bases 2354..2373"
FT misc_binding 5914..5914
FT /note="SIT unique AvaII"
FT misc_binding 6002..6002
FT /note="SIT unique NarI"
FT promoter 0..0
FT /note="PRO E. coli lac operator region"
FT CDS <6228..<6935
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 forward -40"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 universal"
FT misc_binding 6228..6293
FT /note="MCS unique EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT SalI-PstI-SphI-HindIII"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 reverse"
FT misc_binding 6408..6408
FT /note="SIT unique PvuI"
FT misc_binding 6437..6437
FT /note="SIT unique BglI"
FT misc_binding 6501..6501
FT /note="SIT unique SauI"
FT misc_binding 0..0
FT /note="SIT unique MstII"
FT misc_binding 6935..6935
FT /note="SIT unique BglII"
XX
SQ Sequence 7249 BP; 1768 A; 1538 C; 1533 G; 2410 T; 0 other;
aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttc
tcgtcagggc aagccttatt cactgaatga gcagctttgt tacgttgatt tgggtaatga
atatccggtt cttgtcaaga ttactcttga tgaaggtcag ccagcctatg cgcctggtct
gtacaccgtt catctgtcct ctttcaaagt tggtcagttc ggttccctta tgattgaccg
tctgcgcctc gttccggcta agtaacatgg agcaggtcgc ggatttcgac acaatttatc
aggcgatgat acaaatctcc gttgtacttt gtttcgcgct tggtataatc gctgggggtc
aaagatgagt gttttagtgt attctttcgc ctctttcgtt ttaggttggt gccttcgtag
tggcattacg tattttaccc gtttaatgga aacttcctca tgaaaaagtc tttagtcctc
aaagcctctg tagccgttgc taccctcgtt ccgatgctgt ctttcgctgc tgagggtgac
gatcccgcaa aagcggcctt taactccctg caagcctcag cgaccgaata tatcggttat
gcgtgggcga tggttgttgt cattgtcggc gcaactatcg gtatcaagct gtttaagaaa
ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt
ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct
attctcactc cgctgaaact gttgaaagtt gtttagcaaa accccataca gaaaattcat
ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggttgtc
tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat
gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt
ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta
ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa
accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc
agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc
aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt
atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgaag
atccattcgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg
ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg
gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg
attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg
aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg
ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg
gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt
taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt
ttgtctttag cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat
tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt
ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt
attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct
taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg
gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt
tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct
ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga
ttgggataaa taatatggct gtttattttg taactggcaa attaggctct ggaaagacgc
tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc
ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc
ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc ggtaatgatt
cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact tggtttaata
cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta catgctcgta
aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat aaacaggcgc
gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt actttacctt
ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct aaattacatg
ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt tggctttata
ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt aattatgatt
ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc aaaccattaa
atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt
gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa cctaagccgg
aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt gactcttctc
agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata
gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt actgtttcca
ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt
gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct gcgcgatttt
gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga tgtaaaaggt
actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt ctttatttct
gttttacgtg ctaataattt tgatatggtt ggttcaattc cttccataat tcagaagtat
aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca ggaatatgat
gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaact
tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt gtttgtaaag
tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct attagttgtt
agtgcaccta aagatatttt agataacctt cctcaattcc tttctactgt tgatttgcca
actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga tgctttagat
ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa tactgaccgc
ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg cgatgtttta
gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgccacgt
attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt cccttttatt
actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac gattgagcgt
caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt
ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt
actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac tcttttactc
ggtggcctca ctgattataa aaacacttct caagattctg gcgtaccgtt cctgtctaaa
atccctttaa tcggcctcct gtttagctcc cgctctgatt ccaacgagga aagcacgtta
tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
caggcggtga agggcaatca gctgttgccc gtctcgctgg tgaaaagaaa aaccaccctg
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg
ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
cggaaagctg gctggagtgc gatcttcctg aggccgatac ggtcgtcgtc ccctcaaact
ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca
atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt cctattggtt
aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
aatttaaata tttgcttata caatcttcct gtttttgggg cttttctgat tatcaaccgg
ggtacatatg attgacatgc tagttttacg attaccgttc atcgattctc ttgtttgctc
cagactctca ggcaatgacc tgatagcctt tgtagatctc tcaaaaatag ctaccctctc
cggcattaat ttatcagcta gaacggttga atatcatatt gatggtgatt tgactgtctc
cggcctttct cacccttttg aatctttacc tacacattac tcaggcattg catttaaaat
atatgagggt tctaaaaatt tttatccttg cgttgaaata aaggcttctc ccgcaaaagt
attacagggt cataatgttt ttggtacaac cgatttagct ttatgctctg aggctttatt
gcttaatttt gctaattctt tgccttgcct gtatgattta ttggatgtt
//