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ID M13MP19 preliminary; circular DNA; SYN; 7250 BP.
XX
AC L08821; VB0019; IG0006;
XX
DT 20-NOV-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector M13mp19 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-7249
RC M13mp18 from M13mp8
RC M13mp19 from M13mp9
RC pUC18 from pUC8
RC pUC19 from pUC9
RA Yanisch-Perron C., Vieira J., Messing J.;
RT "Improved M13 phage cloning vectors and host strains: nucleotide
RT sequences of the M13mp18 and pUC19 vectors";
RL Gene 33:103-119(1985).
XX
RN [2]
RP 1-7250
RC M13mp18 from M13mp8
RC M13mp19 from M13mp9
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
RN [3]
RC M13mp18 from M13mp10
RC M13mp19 from M13mp11
RA Norrander J., Kempe T., Messing J.;
RT "Construction of improved M13 vectors using
RT oligodeoxynucleotide-directed mutagenesis";
RL Gene 26:101-106(1983).
XX
CC M13 is not lytic; the phage extrude through the cell wall.
CC The replicative RF I form in the cell is double-stranded and
CC circular, and acts like a plasmid.
CC The phage form outside the cell is linear and single-stranded.
CC NM (M13mp19)
CC CM (yes)
CC NA (filamentous ss-DNA)(ds-DNA)
CC TP (linear)(circular)
CC ST ()
CC TY (phage)
CC SP (Boehringer)(Bio-Rad)(BRL)(IBI)(NEB)(Pharmacia)(Sigma)
CC HO (E.coli JM101)(E.coli JM103)(E.coli JM105)(E.coli CJ236)
CC HO (E.coli JM107)(E.coli JM109)(E.coli DH5alphaF')(E.coli NM522)
CC CP ()
CC FN (cloning oriented)(sequencing dideoxy)(in vitro mutagenesis)
CC FN (labeled strand-specific DNA)
CC SE (color blue/white)
CC PA (M13mp9)(M13mp11)
CC BR (M13mp18)
CC OF (pUC19)(pUC19c)(PhageScript SK)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. M13mp11 7244bp, MCS
FT 2. oligo SphI-KpnI 12bp gcatgcggtacc
FT -> M13mp19 7250bp"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene II (rf replication
FT and nicking)"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene X"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene V (single stranded
FT binding protein)"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene IX (minor coat
FT protein)"
FT misc_binding 0..0
FT /note="SIT unique SnaBI"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene VIII (major coat
FT protein)"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene III (adsorption
FT protein)"
FT misc_binding 0..0
FT /note="SIT unique BsmI"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT CDS 0..0
FT /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage M13 intergenic region
FT (M13IG)"
FT misc_binding 0..0
FT /note="SIT unique NaeI"
FT misc_binding 0..0
FT /note="SIT unique DraIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT from pBR322 bases 2354..2373"
FT misc_binding 0..0
FT /note="SIT unique AvaII"
FT misc_binding 0..0
FT /note="SIT unique NarI"
FT promoter 0..0
FT /note="PRO E. coli lac operator region"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 forward -40"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 universal"
FT misc_binding 6234..6290
FT /note="MCS unique HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT SmaI-KpnI-SacI-EcoRI"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 reverse"
FT misc_binding 0..0
FT /note="SIT unique BglI"
FT misc_binding 0..0
FT /note="SIT unique SauI"
FT misc_binding 0..0
FT /note="SIT unique MstII"
FT misc_binding 0..0
FT /note="SIT unique BglII"
FT misc_feature 1..5868
FT /note="from bacteriophage M13"
FT misc_feature 5869..6231
FT /note="from E. coli lac operon"
FT misc_feature 6234..6290
FT /note="MCS M13MP19/pUC19 polylinker"
FT misc_feature 6291..6711
FT /note="from E. coli lac operon"
FT misc_feature 6712..7250
FT /note="from bacteriophage M13"
XX
SQ Sequence 7250 BP; 1768 A; 1540 C; 1531 G; 2411 T; 0 other;
aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc
gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
ccggaaagct ggctggagtg cgatcttcct gaggccgata cggtcgtcgt cccctcaaac
tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc
aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt tcctattggt
taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga ttatcaaccg
gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct cttgtttgct
ccagactctc aggcaatgac ctgatagcct ttgtagatct ctcaaaaata gctaccctct
ccggcattaa tttatcagct agaacggttg aatatcatat tgatggtgat ttgactgtct
ccggcctttc tcaccctttt gaatctttac ctacacatta ctcaggcatt gcatttaaaa
tatatgaggg ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag
tattacaggg tcataatgtt tttggtacaa ccgatttagc tttatgctct gaggctttat
tgcttaattt tgctaattct ttgccttgcc tgtatgattt attggatgtt
//