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ID M13TG131 preliminary; circular DNA; SYN; 7265 BP.
XX
AC L08833; VB0055; IG0011; K01159;
XX
DT 20-NOV-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector M13tg131 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-7265
RC M13tg131
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
RN [2]
RC ptg130 from pBR322 & oligo
RC M13tg130 from M13tg109 & ptg130
RC M13tg115 from linker & M13tg110
RC M13tg119 from M13tg115 & M13mp701
RC M13tg120 from M13tg119 & M13mp701 & M13mp7
RC M13tg131 from M13tg130 & M13tg120
RA Kieny M.P., Lathe R.F., Lecocq J.P.;
RT "New versatile cloning and sequencing vectors based on
RT bacteriophage M13";
RL Gene 26:91-99(1983).
XX
CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Assembled from M13mp7 and M13tg131-Polylinker by F. Pfeiffer.
CC The BglII site in the polylinker region is not unique.
CC M13 is not lytic; the phage extrude through the cell wall.
CC The replicative RF I form in the cell is double-stranded and
CC circular, and acts like a plasmid.
CC The phage form outside the cell is linear and single-stranded.
CC A series of deletions and replacements into the polylinker
CC of M13mp7 creates the final polylinker.
CC Amersham vector is 7195 bp.
CC NM (M13tg131)
CC CM (yes)
CC NA (filamentous ss-DNA)(ds-DNA)
CC TP (linear)(circular)
CC ST ()
CC TY (phage)
CC SP (Amersham)
CC HO (E.coli JM101)(E.coli JM103)(E.coli JM105)
CC HO (E.coli JM107)(E.coli JM109)(E.coli DH5alphaF')
CC CP ()
CC FN (cloning)(sequencing dideoxy)(in vitro mutagenesis)
CC FN (labeled strand-specific DNA)
CC SE (color blue/white)
CC PA (M13mp7)
CC BR (M13tg130)
CC OF (pUC831)(pOM1)(pOM3)(pOM9)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. M13tg120 remove small PstI-EcoRI, 7200bp
FT 2. M13tg130 EcoRI-PstI 67bp 6232..6299, MCS
FT -> M13tg131 7265bp"
FT CDS join(6864..7195,1..831)
FT /note="GEN bacteriophage M13 gene II (rf replication
FT and nicking)"
FT CDS 496..831
FT /note="GEN bacteriophage M13 gene X"
FT CDS 843..1106
FT /note="GEN bacteriophage M13 gene V (single stranded
FT binding protein)"
FT CDS 1108..1209
FT /note="GEN bacteriophage M13 gene VII (morphogenesis)"
FT CDS 1206..1304
FT /note="GEN bacteriophage M13 gene IX (minor coat
FT protein)"
FT CDS 1301..1522
FT /note="GEN bacteriophage M13 gene VIII (major coat
FT protein)"
FT CDS 1579..2853
FT /note="GEN bacteriophage M13 gene III (adsorption
FT protein)"
FT CDS 2856..3194
FT /note="GEN bacteriophage M13 gene VI (morphogenesis)"
FT CDS 3196..4242
FT /note="GEN bacteriophage M13 gene I (morphogenesis)"
FT CDS 4220..5500
FT /note="GEN bacteriophage M13 gene IV (morphogenesis)"
FT rep_origin complement(5488..5868)
FT /note="ORI bacteriophage M13 intergenic region
FT (M13IG)"
FT promoter 6095..6216
FT /note="PRO E. coli lac operator region"
FT CDS 6217..6699
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 -40"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 universal"
FT primer_bind 0..0
FT /note="PRI bacteriophage M13 reverse"
FT misc_feature 1..6232
FT /note="from M13mp7"
FT misc_feature 6233..6302
FT /note="MCS (BglII)-PstI-SalI-BamHI-HindIII-XbaI-
FT KpnI-SphI-EcoRV-SstI-SmaI-EcoRI"
FT misc_feature 6306..7265
FT /note="from M13mp7"
XX
SQ Sequence 7265 BP; 1772 A; 1543 C; 1534 G; 2416 T; 0 other;
aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gcagatctgc
aggtcgacgg atcccaagct tcttctagag gtaccgcatg cgatatcgag ctctcccggg
aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatacggtc
gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
ggcgttccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt
tctgattatc aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg
attctcttgt ttgctccaga ctctcaggca atgacctgat agcctttgta gatctctcaa
aaatagctac cctctccggc attaatttat cagctagaac ggttgaatat catattgatg
gtgatttgac tgtctccggc ctttctcacc cttttgaatc tttacctaca cattactcag
gcattgcatt taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg
cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat
gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg
atgtt
//