Return to this vector's summary.
ID PAA113M preliminary; circular DNA; SYN; 9186 BP.
XX
AC M20189;
XX
DT 25-APR-1990 (Rel. 5, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli cosmid vector pAA113M - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-9186
RC pAA113M from pAA113X & pZ152, M13 ori
RA Ahmed A.;
RT "A vector for sequencing long (40-kb) DNA fragments";
RL Gene 75:315-321(1989).
XX
RN [2]
RC pAA113M
RA Ahmed A.;
RT ;
RL Submitted (28-SEP-1988) to GenBank on computer-readable media by:
RL Ahmed A., .
XX
RN [3]
RC delta113 from pAA3.7
RC pAA113X from delta113
RA Ahmed A.;
RT "A simple and rapid procedure for sequencing long (40-kb) DNA
RT fragments";
RL Gene 61:363-372(1987).
XX
CC The cos site is used to clone long segments.
CC The IS1 is used to subdivide the cloned fragment by deletions.
CC The galKT is used to select such deletions.
CC The unique restriction sites are used to further shorten the fragment
CC for supercoil or M13 sequencing.
CC It contains a normal plasmid origin of replication and also
CC the M13 ori of replication to obtain double- or single-stranded DNA.
CC NM (pAA113M)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (sequencing up to 40000 bp)(supercoiling)
CC SE (color red/white)
CC PA (M13)(lambda)(E.coli)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pAA3.7 remove IS1-L to cos site, galS gene
FT -> delta113
FT 1. delta113 remove BglII-HpaI, cos/galK
FT fill in:
FT -> pAA113X 8749bp
FT 1. pAA113X large ScaI-BamHI
FT 2. pZ152 large ScaI-BamHI, M13 ori
FT -> pAA113M 9186bp"
FT CDS 3787..4977
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT CDS 0..0
FT /note="GEN E. coli galactokinase gene (galKT)"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cos region"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT rep_origin 0..0
FT /note="ORI bacteriophage M13 intergenic region"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_feature 0..0
FT /note="IS1 region"
FT misc_binding 0..0
FT /note="MCS"
XX
SQ Sequence 9186 BP; 2051 A; 2446 C; 2453 G; 2236 T; 0 other;
caattactgc aatgccctcg taattaagtg aatttacaat atcgtcctgt tcggagggaa
gaacgcggga tgttcattct tcatcacttt taattgatgt atatgctctc ttttctgacg
ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgct
caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg
ttctgcgggt tctgttcttc gttgacatga ggttgccccg tattcagtgt cgctgatttg
tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat
aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc
gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca
taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa
gcgaggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg
gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac
tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat
gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag
ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatcaaca gtcggtacgg
ctgaccatcg ggtgccagtg cgggagtttc gttcagcact gtcctgctcc ttgtgatggt
ttacaaacgt aaaaagtctc tttaatacct gtttttgctt catattgttc agcgacacgt
tgctgtacgg caggcaccag ctcttccggg atcagcgcga cgatacagcc gccaaatccg
ccgccggtca tgcgtacgcc acctttgtcg ccaatcacag ctttgacgat ttctaccaga
gtgtcaattt gcggcacggt gatttcgaaa tcatcgcgca tagaggcatg agactccgcc
atcaactcgc ccatacgttt caggtcgcct tgctccagcg cgctggcagc ttcaacggtg
cgggcgtttt cagtcagtat atgacgcacg cgttttgcca cgatcgggtc cagttcatgc
gcaacagcgt tgaactcttc aatggtgaca tcacgcaggg ctggctgctg gaagaaacgc
gcaccggttt cgcactgttc acgacgggtg ttgtattcgc tgccaaccag ggtacgtttg
aagttactgt tgatgatgac gacagccaca cctttgggca tggaaactgc tttggtcccc
agtgagcggc aatcgatcag caaggcatga tctttcttgc cgagcgcgga aattagctga
tccatgatcc cgcagttaca gcctacaaac tggttttctg cttcctgacc gttaagcgcg
atttgtgcgc cgtccagcgg cagatgataa agctgctgca atacggttcc gaccgcgact
tccagtgaag cggaagaact taacccggca ccctgcggca cattgccgtg atcaaccatg
tccacgccgc cgaagctgtt gttacgcagt tgcagatgtt tcaccacgcc acgaacgtag
ttagcccatt gatagttttc atgtgcgaca atgggcgcat cgagggaaaa ctcgtcgagc
tgattttcat aatcggctgc catcacgcga actttacggt catcgcgtgg tgcacaactg
atcacggttt gataatcaat cgcgcagggc agaacgaaac cgtcgttgta gtcggtgtgt
tcaccaatca aattcacgcg gccaggcgcc tgaatggtgt gagtggcagg gtagccaaat
gcgttggcaa acagagattg tgttttttct ttcagactca tttcttacac tccggattcg
cgaaaatgga tatcgctgac tgcgcgcaaa cgctctgctg cctgttctgc ggtcaggtct
cgctgggtct ctgccagcat ttcataacca accataaatt tacgtacggt ggcggacggc
agcagaggcg gataaaagtg cgcgtgcagc tgccagtgtt gattctcttc gccattaaat
ggcgcgccgt gccagcccat agagtagggg aaggagcact ggaagaggtt gtcataacga
ctggtcagct ttttcaacgc cagcgccaga tcgctgcgct gggcgtcggt caaatcggtg
atccgtaaaa cgtgggcttt gggcagcagt agcgtttcga acggccaggc agcccagtaa
ggcacgacgg ctaaccagtg ttcggtttcg acaacggtac ggctaccgtc tgccagctcg
cgctgaacat aatccaccag cattggtgat ttctgttcgg caaaatattc tttttgcagg
cggtcttcgc gctcagcttc gttaggcagg aagctatttg cccaaatctg accgtgcgga
tgcgggttag agcagcccat cgccgcgcct ttgttttcaa aaacctgcac ccatgggtac
gttttcccca gttctgcggt ttgctcctgc caggttttga cgatttccgt caatgctgca
acgctgagct ctggcagcgt tttactgtga tccggtgaaa agcagatcac ccggctggtg
ccgcgcgcgc tctggcaacg catcagcgga tcgtgacttt ctggcgcatc tggcgtgtca
gacatcaaag ccgcaaagtc attagtgaaa acgtaagtcc cggtgtaatc ggggttttta
tcgcctgtca cccgcacatt acctgcgcag aggaagcaat ctggatcgtg cgcaggtaac
acctgtttgg ctggcgtttc ctgcgccccc tgccagggcg ttagccgggt gcggtgaaac
cagaatccat tgcccggtga gcggttgtag cggcgatgtg gatgatcaac gggattaaat
tgcgtcatgg tcgttcctta atcgggatat ccctgtggat ggcgtgactg ccagtgccag
gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcagt tcagttcacg gtcggctttg
ctggcgtccg ccagtaggcc ggaaggtcgc cctcgcgagc ggtgcaaaat gataattaac
cggtttgccg cacgtttgct gaaggcatta accacgtcca gcacgctgtt gcctacgcca
gcgccgaggt tgtagatgtg tacgcctggc ttgttcgcca gtttttccat cgccacgacg
tgaccgtccg ccagatccat tacgtggatg taatcgcgta cgccagtacc atcttcggtc
ggataatcgt taccaaaaat cgccagcgag tcgcgacggc ctacagcaac ctgggcgatg
tatggcatca ggttattcgg aatgccttgc ggatcttcgc ccatatcgcc cgacggatgc
gcgccaaccg ggttgaagta gcgcacgagg gcaatgctcc agtccggctg ggctttttgc
agatcggtga ggatctgttc caccatcagc ttgcttttgc cgtaagggct ttgcggtgtg
ccggtcggga agctataatg cggtagttta tcacagttaa attgctaacg cagtcaggca
ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc
gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc
ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac
ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca
tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
agattatcaa aaaggatctt cacctagatc cttttaaatt gtaaacgtta atattttgtt
aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg ttccagtttg
gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg ggtcgaggtg
ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
acagggcgcg taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcgg taatgactcc aacttattga tagtgtttta tgttcagata
atgcccgatg actttgtcat gcagctccac cgattttgag aacgacagcg acttccgtcc
cagccgtgcc aggtgctgcc tcagattcag gttatgccgc tcaattcgct gcgtatatcg
cttgctgatt acgtgcagct ttcccttcag gcgggattca tacagcggcc agccatccgt
catccatatc accacgtcaa agggtgacag caggctcata agacgcccca gcgtcgccat
agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa
cagccagcgc tggcgcgatt tagccccgac atagccccac tgttcgtcca tttccgcgca
gacgatgacg tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt
aagtgacgta aaatcgtgtt gaggccaacg cccataatgc gggctgttgc ccggcatcca
acgccattca tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa
gtgaactgca gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg
gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
atcacc
//