Return to this vector's summary.
ID PAC360 preliminary; circular DNA; SYN; 9828 BP.
XX
AC IG1022; A13228; A13229; A13230;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pAc360 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pAc101, pAc311, pAc360 from AcMNPV
RC pAc373 from AcMNPV
RC pAc373-CAT from pAc373
RC pAc401, pAc409, pAc436, pAc461 from pAc311
RC pAc461-RI-, pAc461-RI/PstI, pAc461-PstI from pAc461
RC pAc510 from AcMNPV
RC pAc610, pAc611 from pAc461
RC pAc700, pAc701, pAc702 from pAc373
RC pAcRP6, pAcRP18 from AcMNPV
RC pAcYM1 from AcMNPV
RC pVL77 from pUC19
RC pVL101 from pAc401
RC pVL106 from pAc436
RC pAcC4, pAcC5 from AcMNPV
RA Luckow V.A., Summers M.D.;
RT "Trends in the development of baculovirus expression vectors";
RL Biotechnology 6:47-55(1988).
XX
RN [2]
RC pI8 from pUC8 & AcNPV EcoRI-1
RC pAc101 from pI8
RC pB' from pUC8 & AcNPV EcoRI-1
RC pB'-mutant from pB' & pUC8
RC pAc311 from pAc101 & pB'-mutant
RC pAc360 from pAc101 & pB'-mutant
RC pAc380 from pAc101 & pB'-mutant
RA Smith G.E., Summers M.D., Fraser M.J.;
RT "Production of human beta interferon in insect cells infected
RT with a baculovirus expression vector";
RL Mol. Cell. Biol. 3:2156-2165(1983).
XX
RN [3]
RC pIL-2-2b from interleukin 2 gene
RC from pAc373 & IL-2 gene
RC from pAc380 & IL-2 gene
RA Smith G.E., Ju G., Ericson B.L., Moschera J., Lahm H., Chizzonite R.,
RA Summers M.D.;
RT "Modification and secretion of human interleukin 2 produced in
RT insect cells by a baculovirus expression vector";
RL Proc. Natl. Acad. Sci. U.S.A. 82:8404-8408(1985).
XX
RN [4]
RC 39-50, 81-268, 83-174, 86-139 from pBR322 & LCMV WE S
RC B-13 from 39-50 & 81-268
RC 86-139-1 from pBR322 & 86-139
RC A-3 from 83-174 & 86-139-1
RC Y-1 from B-13 & A-3
RC Y-1-A from pBR322 & Y-1
RC pAcRP1-LCM WE G, pAcRP1-LCM WE N from pAcRP1 & Y-1-A
RC pAcRP5-LCM WE G, pAcRP5-LCM WE N from pAcRP5 & Y-1-A
RC pAcRP6-LCM WE G, pAcRP6-LCM WE N from pAcRP6 & Y-1-A
RC pAcRP5-LCM WE S from pAcRP5-LCM WE G & pAcRP5-LCM WE N
RC YN6, YN12, YN13 from pAcRP5-LCM WE S
RC YG5 from pAcRP5-LCM WE G
RC A251 from pAcRP1-LCM WE N
RA Matsuura Y., Possee R.D., Bishop D.H.L.;
RT "Expression of the S-coded genes of lymphocytic choriomeningitis
RT arenavirus using a baculovirus vector";
RL J. Gen. Virol. 67:1515-1529(1986).
XX
RN [5]
RC pEVIV from pUC8 & AcMNPV, polyhedrin gene
RC pEV/55 from pEVIV
RC pEV/cc from pEV/55
RA Miller D.W., Safer P., Miller L.K.;
RT "An insect baculovirus host-vector system for high-level
RT expression of foreign genes";
RL Genet. Eng. 8:277-298(1986).
XX
CC NM (pAc360)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(insect)
CC CP ()
CC FN (expression)(transfer)
CC SE ()
CC PA ()
CC BR ()
CC OF (pVL1392)(pVL1393)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC8 EcoRI 2665bp 231..231, MCS
FT 2. AcNPV EcoRI-EcoRI, polyhedrin gene EcoRI fragment 1
FT -> pI8
FT 1. pI8 PstI-SmaI, in pUC8 part
FT S1 nuclease
FT BamHI, 3' to polyhedrin gene
FT S1 nuclease
FT -> pAc101 [unique BamHI in polyhedrin]
FT 1. pUC8 remove EcoRI-BamHI 10bp 231.241, MCS/2655bp
FT 2. AcNPV EcoRI-BamHI, EcoRI fragment 1 0..4210
FT -> pB'
FT 1. pB' BamHI
FT BAL31 nuclease
FT Klenow:Klenow
FT BamHI linker 8bp cggatccg:BamHI linker 8bp cggatccg
FT BamHI-EcoRI
FT 2. pUC8 remove EcoRI-BamHI 10bp 231.241, MCS/2655bp
FT -> pB'-mutant
FT 1. pAc101 XhoI-BamHI 2310bp, EcoRI frag 1 1900..4210
FT 2. pB'-mutant XhoI-BamHI
FT -> pAc360 9828bp"
FT transposon 0..0
FT /note="TRN AcMNPV"
FT misc_binding 868..868
FT /note="SIT SacII"
FT misc_binding 1395..1395
FT /note="SIT ApaI"
FT misc_binding 1901..1901
FT /note="SIT XhoI"
FT misc_binding 3998..3998
FT /note="SIT EcoRV"
FT promoter 0..0
FT /note="PRO AcMNPV polyhedrin gene"
FT misc_binding 4128..4128
FT /note="SIT BamHI"
FT misc_binding 4588..4588
FT /note="SIT KpnI"
FT misc_binding 7194..7194
FT /note="SIT EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
XX
SQ Sequence 9828 BP; 2679 A; 2153 C; 2210 G; 2786 T; 0 other;
aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
aaacctataa atatgccgga ttattcatac cgtcccacca tcgggccgga tcctttcctg
ggacccggca agaaccaaaa actcactctc ttcaaggaaa tccgtaatgt taaacccgac
acgatgaagc ttgtcgttgg atggaaagga aaagagttct acagggaaac ttggacccgc
ttcatggaag acagcttccc cattgttaac gaccaagaag tgatggatgt tttccttgtt
gtcaacatgc gtcccactag acccaaccgt tgttacaaat tcctggccca acacgctctg
cgttgcgacc ccgactatgt acctcatgac gtgattagga tcgtcgagcc ttcatgggtg
ggcagcaaca acgagtaccg catcagcctg gctaagaagg gcggcggctg cccaataatg
aaccttcact ctgagtacac caactcgttc gaacagttca tcgatcgtgt catctgggag
aacttctaca agcccatcgt ttacatcggt accgactctg ctgaagagga ggaaattctc
cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact
ggtccggcgt attaaaacac gatacattgt tattagtaca tttattaagc gctagattct
gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt aacatcgggc
gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg
ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca
ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat
aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt
ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc
tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga
tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt
tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt
ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata
atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc
tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta
taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc
gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag
ctcgccgcac gccgataaca agccttttca tttttactac agcattgtag tggcgagaca
cttcgctgtc gtcgacgtac atgtatgctt tgttgtcaaa aacgtcgttg gcaagcttta
aaatatttaa aagaacatct ctgttcagca ccactgtgtt gtcgtaaatg ttgtttttga
taatttgcgc ttccgcagta tcgacacgtt caaaaaattg atgcgcatca attttgttgt
tcctattatt gaataaataa gattgtacag attcatatct acgattcgtc atggccacca
caaatgctac gctgcaaacg ctggtacaat tttacgaaaa ctgcaaaaac gtcaaaactc
ggtataaaat aatcaacggg cgctttggca aaatatctat tttatcgcac aagcccacta
gcaaattgta tttgcagaaa acaatttcgg cgcacaattt taacgctgac gaaataaaag
ttcaccagtt aatgagcgac cacccaaatt ttataaaaat ctattttaat cacggttcca
tcaacaacca agtgatcgtg atggactaca ttgactgtcc cgatttattt gaaacactac
aaattaaagg cgagctttcg taccaacttg ttagcaatat tattagacag ctgtgtgaag
cgctcaacga tttgcacaag cacaatttca tacacaacga cataaaactc gaaaatgtct
tatatttcga agcacttgat cgcgtgtatg tttgcgatta cggattgtgc aaacacgaaa
actcacttag cgtgcacgac ggcacgttgg agtattttag tccggaaaaa attcgacaca
caactatgca cgtttcgttt gactggtacg cggcgtgtta acatacaagt tgctaaccgg
cggccgacac ccatttgaaa aaagcgaaga cgaaatgttg gacttgaata gcatgaagcg
tcgtcagcaa tacaatgaca ttggcgtttt aaaacacgtt cgtaacgtta acgctcgtga
ctttgtgtac tgcctaacaa gatacaacat agattgtaga ctcacaaatt acaaacaaat
tataaaacat gagtttttgt cgtaaaaatg ccacttgttt tacgagtaga attgaattcg
taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgcc
//