Return to this vector's summary.
ID PACUW31 preliminary; circular DNA; SYN; 8532 BP.
XX
AC IG1023;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pAcUW31 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pAcRP19 from AcMNPV; [similar to pAcRP18]
RC pAcRP19.HA from pAcRP19 & A/PR/8/34 HA gene
RC pAcRP25-BglII from pAcRP25 & linker
RC pAcRP25-BglII-p10 from pAcRP25-BglII & pAcp10+1
RC pAcUW3 from pAcRP25-BglII-p10 & pAcUW2
RA Weyer U., Possee R.D.;
RT "A baculovirus dual expression vector derived from the Autographa
RT californica nuclear polyhedrosis virus polyhedrin and p10
RT promoters: co-expression of two influenza virus genes in insect
RT cells";
RL J. Gen. Virol. 72:2967-2974(1991).
XX
RN [2]
RC pBR322.BTV-10.7, pBR322.BTV-17.3 from pBR322 & BTV
RC pUC4.BTV-10.7 from pBR322.BTV-10.7 & pUC4K
RC pUC4.BTV-17.3 from pBR322.BTV-17.3 & pUC4K
RC pAcVC3.BTV-10.7 from pUC4.BTV-10.7 & pAcVC3
RC pAcVC3.BTV-10.7.BTV-17.3 from pUC4.BTV-17.3 & pAcVC3.BTV-10.7
RA French T.J., Roy P.;
RT "Synthesis of bluetongue virus (BTV) corelike particles by a
RT recombinant baculovirus expressing the two major structural core
RT proteins of BTV";
RL J. Virol. 64:1530-1536(1990).
XX
RN [3]
RC pAcUW31 from pAcUW3
RA Lopez-Ferber M., Possee R.D.;
RT ;
RL Unpublished (1992).
XX
RN [4]
RC pAcEcoRI from pUC8 & AcNPV
RC plasmid from pAcYM1 & pAcEcoRI
RC pAcVC2 from plasmid & LCMV N protein gene
RC [pAcVC3 from pAcVC2]
RA Emery V.C., Bishop D.H.;
RT "The development of multiple expression vectors for the high level
RT synthesis of eukaryotic proteins: expression of LCMV-N and AcNPV
RT polyhedrin protein by a recombinant baculovirus";
RL Protein Engineering 1:359-366(1987).
XX
CC NM (pAcUW31)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (CLONTECH)
CC HO (Spodoptera frugiperda IPLB-SF21)
CC CP ()
CC FN (expression)(transfer)
CC SE ()
CC PA (pAcUW3)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pAcRP25 EcoRV
FT BglII linker 6bp agatct
FT -> pAcRP25-BglII
FT 1. pAcRP25-BglII BglII
FT 2. pAcp10+1 BglII-BamHI 230bp, p10 promoter
FT -> pAcRP25-BglII-p10
FT 1. pAcRP25-BglII-p10 BglII-SnaBI 427bp, p10/polyh prom
FT 2. pAcUW2 remove BglII-SnaBI 1170bp, polyh
FT -> pAcUW3 10040bp [BamHI in polyh prom, BglII in p10]
FT 1. pAcUW3
FT -> pAcUW31 8532bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT transposon 0..0
FT /note="TRN AcMNPV"
FT misc_binding 235..235
FT /note="SIT SphI"
FT misc_binding 1256..1256
FT /note="SIT BstXI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 2552..2552
FT /note="SIT EcoRI"
FT misc_binding 2558..2558
FT /note="SIT BglII"
FT promoter 0..0
FT /note="PRO AcMNPV p10 gene"
FT misc_binding 2797..2797
FT /note="SIT XbaI"
FT promoter 0..0
FT /note="PRO AcMNPV polyhedrin gene"
FT misc_binding 2918..2918
FT /note="SIT BamHI"
FT polyA_signal 0..0
FT /note="PLA AcMNPV polyhedrin gene"
FT misc_binding 3352..3352
FT /note="SIT HindIII"
FT misc_binding 4398..4398
FT /note="SIT HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage M13"
FT misc_binding 5889..5889
FT /note="SIT DraIII"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 6804..6804
FT /note="SIT ScaI"
FT misc_binding 7764..7764
FT /note="SIT AlwNI"
XX
SQ Sequence 8532 BP; 2370 A; 1823 C; 1899 G; 2440 T; 0 other;
tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc
agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
ctgcaataaa caagttccga gtttgtcaga aagcagacca aacagcggtt ggaataatag
cgagaacaga gaaatagcgg caaaaataat acccgtatca cttttgctga tatggttgat
gtcatgtagc caaatcggga aaaacgggaa gtaggctccc atgataaaaa agtaaaagaa
aaagaataaa ccgaacatcc aaaagtttgt gttttttaaa tagtacataa tggatttcct
tacgcgaaat acgggcagac atggcctgcc cggttattat tatttttgac accagaccaa
ctggtaatgg tagcgaccgg cgctcagctg gaattcagat ctgtgattgt aaataaaatg
taatttacag tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa
ctataatata ttgtgttggg ttgaattaaa ggtcccggca tcctcaaatg cataatttca
tagtccccct tgttgtaagt gatgcgtatt tctgaatctt tgtaaaatag cacacaagac
tccaacgcgt ttggcgtttt attttcttgc tcgactctag aggatcgatc ccccggatct
gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
cgtaacagtt ttgtaataaa aaaacctata aatatcggat ccgggttatt agtacattta
ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata aagcttggac
atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg
cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt
agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg
tggtggagct gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg
cggaggcgga ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg
caacacagtc ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg
tctgagacga gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc
taaaggtgca gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat
cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg
cggtgccgcc ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg
cgcaggcgcc gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg
tggcaattca atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat
ttcgctatcg tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta
aagagattgt ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta
ctacagcatt gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt
caaaaacgtc gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg
tgttgtcgta aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa
attgatgcgc atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat
atctacgatt cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg
aaaactgcaa aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat
ctattttatc gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca
attttaacgc tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa
aaatctattt taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact
gtcccgattt atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca
atattattag acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca
acgacataaa actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg
attacggatt gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt
ttagtccgga aaaaattcga cacacaacta tgcacgtttc gtttgactgg tacgcggcgt
gttaacatac aagttgctaa ccggcggccg acacccattt gaaaaaagcg aagacgaaat
gttggacttg aatagcatga agcgtcgtca gcaatacaat gacattggcg ttttaaaaca
cgttcgtaac gttaacgctc gtgactttgt gtactgccta acaagataca acatagattg
tagactcaca aattacaaac aaattataaa acatgagttt ttgtcgtaaa aatgccactt
gttttacgag tagaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
tgattacgaa tt
//