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ID PACYC177 preliminary; circular DNA; SYN; 3941 BP.
XX
AC X06402; L08776; ATCC37031;
XX
DT 02-APR-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pACYC177 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-3940
RC pACYC177
RA Rose R.E.;
RT ;
RL Submitted (15-DEC-1987) on tape to the EMBL Data Library by:
RL Rose R.E., Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA.
XX
RN [2]
RP 1-3940
RC pACYC177 from pACYC175
RA Rose R.E.;
RT "The nucleotide sequence of pACYC177";
RL Nucleic Acids Res. 16:356-356(1988).
XX
RN [3]
RP 1-1226, 1227-1227, 1228-3940
RC pACYC177
RA Mok Y.K.;
RT ;
RL Submitted (02-MAR-1991) on paper to the EMBL Data Library by:
RL Mok Y.K., .
XX
RN [4]
RP 1-3940
RC from p15A
RC from pACYC177
RC from pACYC184
RA Mok Y.K., Clark D.R., Kam K.M., Shaw P.C.;
RT "BsiY I, a novel thermophilic restriction endonuclease that
RT recognizes 5' ccnnnnnnngg 3' and the discovery of a wrongly
RT sequenced site in pACYC177";
RL Nucleic Acids Res. 19:2321-2323(1991).
XX
RN [5]
RC pACYC139 from p15A & pSC105
RC pACYC140 from p15A & pSC105
RC pACYC142 from pACYC140 & pSC204, Tn3
RC pACYC160 from p15A & pSC101
RC pACYC161 from pACYC160 & pSC212
RC pACYC175 from pACYC161 & pKT002
RC pACYC177 from pACYC175
RC pACYC184 from pACYC175
RA Chang A.C., Cohen S.N.;
RT "Construction and characterization of amplifiable multicopy DNA
RT cloning vehicles derived from the P15A cryptic miniplasmid";
RL J. Bacteriol. 134:1141-1156(1978).
XX
RN [6]
RP 1-3941
RC pACYC177
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
RN [7]
RC pKT007 from R6 & pML21
RC pKT002 from ColE1 & R6-5
RC pFC001 from ColE1 & R6-5
RC pFC012 from ColE1 & R6-5
RA Timmis K., Cabello F., Cohen S.N.;
RT "Properties and regulation of DNA replication origins and
RT associated functions in multi-origin replicons";
RL Plasmids 0:417-424(1977).
RL Mitsuhashi S., ed., Avicenum, Prague.
XX
RN [8]
RC pSC122 from pSC113
RC pSC137, pSC138, pSC140, pSC141 from pSC122 & F' lac
RC pSC135, pSC136, pSC139 from pSC122 & R6-5
RA Timmis K., Cabello F., Cohen S.N.;
RT "Cloning, isolation, and characterization of replication regions
RT of complex plasmid genomes";
RL Proc. Natl. Acad. Sci. U.S.A. 72:2242-2246(1975).
XX
RN [9]
RC from ColE1
RC from ColE2
RC from ColV
RC from ColIb
RA Helinski D.R., Herschman H.R.;
RT "Effect of Rec- mutations on the activity of colicinogenic factors";
RL J. Bacteriol. 94:700-706(1967).
XX
RN [10]
RC pSC204 from pSC201 & pSC50, Tn3
RC pSC301 from pSC101
RC pSC304 from pSC101 & Tn3
RA Kretschmer P.J., Cohen S.N.;
RT "Selected translocation of plasmid genes: frequency and regional
RT specificity of translocation of the Tn3 element";
RL J. Bacteriol. 130:888-899(1977).
XX
RN [11]
RC pSC211, pSC213, pSC215, pSC217, pSC220 from pSC105 & pSC50, Tn3
RC pSC221, pSC222 from pSC105 & pSC50, Tn3
RC PCC10 from pSC105 & Tn21
RC PCC25 from pSC105 & Tn21
RC PCC36 from pSC105 & Tn21
RC pACYC140 from pSC105
RC [pACYC144 from pSC105]
RC pSC212, pSC225, pSC226 from pSC211
RC pSC214, pSC219A, pSC219B, pSC223, pSC224 from pSC213
RC pSC208 from pSC220
RC pSC209 from pSC221
RA Nisen P.D., Kopecko D.J., Chou J., Cohen S.N.;
RT "DNA sequence deletion at the termini of a transposable
RT ampicillin-resisatnt element";
RL J. Mol. Biol. 117:975-998(1977).
XX
RN [12]
RC pSC113 from pSC101 & pI258
RA Chang A.C., Cohen S.N.;
RT "Genome construction between bacterial species in vitro:
RT replication and expression of Staphylococcus plasmid genes in
RT Escherichia coli";
RL Proc. Natl. Acad. Sci. U.S.A. 71:1030-1034(1974).
XX
CC The sequence is circular; however, the sequence starts with a HincII
CC site.
CC Bases 564-841, 1494-1721, and 2869-3039 were determined
CC while the rest of the sequence was taken from the published
CC sequences of Tn903, Tn3, and p15A.
CC Medium is 1227 LB plus ampicillin.
CC NM (pACYC177)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (NEB)(ATCC)
CC HO (E.coli 1200)(E.coli C600 chi1776)(E.coli)
CC CP (low)
CC FN (cloning)
CC SE ()
CC PA (Tn3)(Tn903)(p15A)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSC101 EcoRI 9263bp 9262..9262
FT 2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT -> pSC105 16163bp
FT 1. p15A HincII
FT 2. pSC105 EcoRI-SalI, Tn903 kan gene/#V00359
FT reverse transcriptase fill in:reverse transcriptase
FT \ fill in
FT -> pACYC140 4600bp
FT 1. R1
FT -> R1-19
FT 1. R1-19, Tn3/Tn4 amp gene
FT -> pSC50
FT 1. pSC105
FT -> pSC205
FT 1. pSC205
FT -> pSC201
FT 1. pSC201
FT 2. pSC50, Tn3 amp gene
FT -> pSC204
FT 1. pACYC140
FT transposition
FT 2. pSC204, Tn3 amp gene
FT -> pACYC142 10800bp
FT 1. pACYC142 remove HaeII-HaeII fragments and religate
FT -> pACYC177 3941bp [amp, kan]"
FT misc_feature complement(3698..3940)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp), from Tn3 [2]"
FT misc_feature complement(1..615)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp), from Tn3 [2]"
FT misc_binding 1..1
FT /note="SIT unique HincII"
FT misc_binding 18..18
FT /note="SIT unique BcgI"
FT misc_binding 62..62
FT /note="SIT unique ScaI"
FT misc_binding 299..299
FT /note="SIT unique PstI"
FT misc_binding 320..320
FT /note="SIT unique FspI"
FT misc_binding 421..421
FT /note="SIT unique BglI"
FT misc_binding 455..455
FT /note="SIT unique BpmI"
FT misc_binding 473..473
FT /note="SIT unique BsaI"
FT misc_binding 524..524
FT /note="SIT unique Pfl108I"
FT misc_binding 540..540
FT /note="SIT unique Eam1105I"
FT misc_binding 592..592
FT /note="SIT unique BanI"
FT misc_feature complement(615..3698)
FT /note="TRN Tn3 with beta-lactamase gene"
FT repeat_unit 728..765
FT /note="TRN Tn3 inverted repeat"
FT misc_feature complement(756..3026)
FT /note="TRN Tn3 fragment [2]"
FT rep_origin 766..1595
FT /note="ORI E. coli miniplasmid p15A [5]"
FT misc_binding 987..987
FT /note="SIT unique AlwNI"
FT misc_binding 1013..1013
FT /note="SIT unique NspI"
FT misc_binding 1096..1096
FT /note="SIT unique BspGI"
FT conflict 1226..1228
FT /note="replace(1226..1228,ag); ref. [1] & [2]"
FT rep_origin 1326..1328
FT /note="ORI E. coli miniplasmid p15A (start) [5]"
FT misc_binding 1338..1338
FT /note="SIT unique SacII"
FT misc_binding 1500..1500
FT /note="SIT unique SgrAI"
FT misc_binding 1505..1505
FT /note="SIT unique BsgI"
FT misc_binding 1576..1576
FT /note="SIT unique AccI"
FT misc_binding 1587..1587
FT /note="SIT unique NheI"
FT repeat_unit 1590..1817
FT /note="TRN Tn903 inverted repeat"
FT misc_feature 1595..3025
FT /note="TRN HaeII-HaeII fragment from Tn903 [2]"
FT misc_binding 1817..1817
FT /note="SIT unique DraIII"
FT misc_feature 1922..2734
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)
FT kanamycin resistance gene (kan), from Tn3 [2]"
FT misc_binding 1951..1951
FT /note="SIT unique XhoI"
FT misc_binding 2004..2004
FT /note="SIT unique BanII"
FT misc_binding 2008..2008
FT /note="SIT unique NruI"
FT misc_binding 2043..2043
FT /note="SIT unique ClaI"
FT misc_binding 2225..2225
FT /note="SIT unique SmaI"
FT misc_binding 2261..2261
FT /note="SIT unique EcoNI"
FT misc_binding 2363..2363
FT /note="SIT unique Esp3I"
FT misc_binding 2471..2471
FT /note="SIT unique HindIII"
FT misc_binding 2610..2610
FT /note="SIT unique PflMI"
FT misc_binding 2790..2790
FT /note="SIT unique BsmFI"
FT repeat_unit 2798..3025
FT /note="TRN Tn903 inverted repeat"
FT misc_feature 3025..3515
FT /note="TRN Tn3 repressor fragment"
FT misc_binding 3278..3278
FT /note="SIT unique BstEII"
FT misc_binding 3319..3319
FT /note="SIT unique BamHI"
FT misc_binding 3396..3396
FT /note="SIT unique DrdI"
FT misc_binding 3469..3469
FT /note="SIT unique FauI"
FT misc_binding 3495..3495
FT /note="SIT unique BbsI"
FT misc_binding 3504..3504
FT /note="SIT unique EcoO109I"
FT misc_binding 3562..3562
FT /note="SIT unique AatII"
FT misc_binding 3798..3798
FT /note="SIT unique Eco57I"
FT misc_binding 3813..3813
FT /note="SIT unique ApaLI"
XX
SQ Sequence 3941 BP; 1026 A; 893 C; 993 G; 1029 T; 0 other;
gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac
ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc
aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg
ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc
ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt
accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag
gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc
gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac
gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg
gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag
ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt
catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg
gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
gcacttttaa agttctgcta tgtggcgcgg tattatcccg t
//