Return to this vector's summary.
ID PACYC184 preliminary; circular DNA; SYN; 4245 BP.
XX
AC X06403; ATCC37033;
XX
DT 02-APR-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pACYC184 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-4244
RC pACYC184
RA Rose R.E.;
RT ;
RL Submitted (15-DEC-1987) on tape to the EMBL Data Library by:
RL Rose R.E., Calgene Inc., 1920 Fifth St., Davis, CA 95616, USA.
XX
RN [2]
RP 1-944, 946-4245
RC pACYC184 from pACYC175
RA Rose R.E.;
RT "The nucleotide sequence of pACYC184";
RL Nucleic Acids Res. 16:355-355(1988).
XX
RN [3]
RC pACYC139 from p15A & pSC105
RC pACYC140 from p15A & pSC105
RC pACYC142 from pACYC140 & pSC204, Tn3
RC pACYC175 from pACYC144
RC pACYC177 from pACYC175
RC pACYC160 from p15A & pSC101
RC pACYC161 from pACYC160 & pSC212
RC pACYC175 from pACYC161 & pKT002
RC pACYC184 from pACYC175
RA Chang A.C., Cohen S.N.;
RT "Construction and characterization of amplifiable multicopy DNA
RT cloning vehicles derived from the P15A cryptic miniplasmid";
RL J. Bacteriol. 134:1141-1156(1978).
XX
RN [4]
RC from lambda
RC from pBR328
RC from pACYC177
RC from pACYC184
RA Li J.K., Tu J.;
RT "Ssp RFI, a novel class-II restriction endonucleasse from
RT Synechococcus RF-1 recognizing 5'TT/CGAA-3'";
RL Nucleic Acids Res. 19:4770-4770(1991).
XX
CC The sequence is circular; however, the sequence begins with a unique
CC EcoRI site.
CC Bases 426-706, 1153-1583, and 2918 to 3735 were determined
CC while the rest of the sequence was taken from the
CC published sequence of pSC101, Tn9, and p15A.
CC Restriction digests of the clone give the following sizes (kb):
CC EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. (ATCC staff)
CC Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC NM (pACYC184)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (NEB)(ATCC)
CC HO (E.coli PM191)(E.coli C600 chi1776)(E.coli)
CC CP (low)
CC FN (cloning)
CC SE ()
CC PA (Tn9)(pSC101)(p15A)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. p15A HincII-HincII 1100bp
FT reverse transcriptase fill in:reverse transcriptase
FT \ fill in
FT 2. pSC101 EcoRI 9263bp 9262..9262
FT -> pACYC160 10400bp
FT 1. pSC101 EcoRI 9263bp 9262..9262
FT 2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT -> pSC105 16200bp
FT 1. R1
FT -> R1-19
FT 1. R1-19, Tn3/Tn4 amp gene
FT -> pSC50
FT 1. pSC105 16200bp
FT 2. pSC50, Tn3 amp gene
FT -> pSC211
FT 1. pSC211
FT -> pSC212 [no tet]
FT 1. pACYC160
FT transposition
FT 2. pSC212
FT -> pACYC161 3000bp
FT 1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT 2. pVH51 EcoRI 3847bp 3846..3846
FT -> pML21 10747bp
FT 1. pML21 10747bp
FT 2. Tn9 HhaI-HhaI 1364bp, cat gene/#V00622
FT \ pBR328 3170..4534
FT -> pKT002
FT 1. pACYC161 HindIII 3000bp, tet gene/pSC101 30..30
FT 2. pKT002 HindIII-PstI 9000bp, cat gene
FT -> pACYC175 12000bp
FT 1. pACYC175 remove small AluI-HaeIII
FT -> pACYC184 4245bp"
FT CDS complement(3804..4244)
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (Cmr)"
FT CDS complement(1..219))
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (Cmr)"
FT misc_binding 1..1
FT /note="SIT unique EcoRI"
FT misc_feature 443..583
FT /note="TRN part of IS1 from Tn9"
FT rep_origin 581..1492
FT /note="ORI E. coli miniplasmid p15A"
FT misc_feature complement(3530..4245, 1..585)
FT /note="TRN Tn9 HaeII fragment 1300bp"
FT misc_binding 594..594
FT /note="SIT unique Bst1107I"
FT misc_binding 635..635
FT /note="SIT unique XmnI"
FT misc_binding 832..832
FT /note="SIT unique SacII"
FT conflict 944..946
FT /note="replace(944..946,tc); ref. [1] & [2]"
FT misc_binding 959..959
FT /note="SIT unique BsiI"
FT misc_binding 1405..1405
FT /note="SIT unique AseI"
FT misc_binding 1424..1424
FT /note="SIT unique XbaI"
FT misc_binding 1446..1446
FT /note="SIT unique EarI"
FT misc_feature 1494..3275
FT /note="pSC101 fragment"
FT misc_binding 1517..1517
FT /note="SIT unique ClaI"
FT misc_binding 1523..1523
FT /note="SIT unique HindIII"
FT CDS 1580..2770
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT misc_binding 1632..1632
FT /note="SIT unique SfcI"
FT misc_binding 1679..1679
FT /note="SIT unique EcoRV"
FT misc_binding 1869..1869
FT /note="SIT unique BamHI"
FT misc_binding 1983..1983
FT /note="SIT unique BspHI"
FT misc_binding 2056..2056
FT /note="SIT unique SphI"
FT misc_binding 2116..2116
FT /note="SIT unique EcoNI"
FT misc_binding 2145..2145
FT /note="SIT unique SalI"
FT misc_binding 2433..2433
FT /note="SIT unique EagI"
FT misc_binding 2466..2466
FT /note="SIT unique NruI"
FT misc_binding 2539..2539
FT /note="SIT unique ApaBI"
FT misc_binding 2557..2557
FT /note="SIT unique BspMI"
FT misc_binding 2919..2919
FT /note="SIT unique AvaI"
FT misc_binding 3175..3175
FT /note="SIT unique DrdII"
FT misc_binding 3203..3203
FT /note="SIT unique Eam1105I"
FT misc_binding 3441..3441
FT /note="SIT unique BsaBI"
FT misc_binding 3541..3541
FT /note="SIT unique BclI"
FT misc_binding 3591..3591
FT /note="SIT unique Bsu36I"
FT misc_binding 3695..3695
FT /note="SIT unique DrdI"
FT misc_binding 3698..3698
FT /note="SIT unique Tth111I"
FT misc_binding 3830..3830
FT /note="SIT unique ScaI"
FT misc_binding 3944..3944
FT /note="SIT unique NcoI"
FT misc_binding 4233..4233
FT /note="SIT unique BsrDI"
XX
SQ Sequence 4245 BP; 956 A; 1167 C; 1094 G; 1028 T; 0 other;
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct
tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga
tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg
gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc
aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta
ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat
gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa
atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg
gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
aacactatcc catatcacca gctcaccgtc tttcattgcc atacg
//