Return to this vector's summary.
ID PAMP10 preliminary; circular DNA; SYN; 4122 BP.
XX
AC IG1311;
XX
DT 01-OCT-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pAMP10 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC [pAMP1, pAMP2, pAMP10 from pSPORT1 & oligo]
RC [pAMP18 from pUC18 & oligo]
RC [pAMP19 from pUC19 & oligo]
RA Eckert K.A., Kunkle T.A.;
RT "DNA polymerase fidelity and the polymerase chain reaction";
RL PCR Meth. Appl. 1:17-24(1991).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pAMP10)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (BRL)
CC HO (E.coli)
CC CP (high)
CC FN (cloning)(expression)(transcription)
CC SE ()
CC PA (pSPORT1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSPORT1 remove NotI-SalI 21bp 233..254,
FT \ MCS 4088bp
FT 2. oligo NotI-XmaIII-SalI 30bp
FT \ ggccgcctactactactaatgatgatgatg
FT -> pAMP10 4118bp"
FT - 1..236
FT /note="pSPORT1 1..236 236bp
FT NotI = GC^GGCC GC
FT \ ggccgcctactactactaatgatgatgatg"
FT - 237..266
FT /note="ggccgcctactactactaatgatgatgatg 30bp
FT \ ggccgcctactactactaatgatgatgatg
FT SalI = G^TCGAC"
FT - 267..4122
FT /note="pSPORT1 254..4109 3856bp"
FT CDS complement(0..0)
FT /note="REP E. coli lacZ gene"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT misc_binding 125..381
FT /note="MCS AatII-SphI-MluI-SunI-SnaBI-HindIII-BamHI-
FT XbaI-NotI-XmaIII-SalI/AccI-SmaI-EcoRI-Kpn2I-RsrII-
FT PinAI-KpnI-Sse8387I-PstI"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT CDS complement(0..0)
FT /note="REP E. coli lacI repressor gene"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
XX
SQ Sequence 4122 BP; 1007 A; 1066 C; 1063 G; 986 T; 0 other;
cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata
gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gcggccggcc
gcctactact actaatgatg atgatgtcga cccgggaatt ccggaccggt acctgcaggc
gtaccagctt tccctatagt gagtcgtatt agagcttggc gtaatcatgg tcatagctgt
ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttgacggcgg gatataacat
gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
ctgacccgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
cgccattcga tggtgtcaac gtaaatgccg cttcgccttc gcgcgcgaat tgcaagctct
gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
caccacaccc gccgcgctta atgcgccgct acagggcgcg tc
//