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ID PAN523 preliminary; circular DNA; SYN; 5670 BP.
XX
AC Z32689;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pAN52-3 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 999-2572
RC M13mAN5-7B from M13mp11 & gpdA gene
RC from pAN5-41B
RC from pAN923-41B
RC from pAN923-42B
RA Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT "Functional elements in the promoter region of the Aspergillus
RT nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT dehydrogenase";
RL Gene 93:101-109(1990).
XX
RN [2]
RP 1-5670
RC pAN series from gpdA gene
RA Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA Van Den Hondel C.A.M.J.J.;
RT "Vectors for efficient production of homologous and heterologous
RT proteins in filamentous fungi";
RL Unpublished (1994).
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RN [3]
RP 1-5670
RC pAN52-3
RA Punt P.;
RT ;
RL Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL Rijkswik, The Netherlands.
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CC NCBI gi: 474929
CC NM (pAN52-3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pAN52-3)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 EcoRI-HindIII 2635bp 451..2686..400
FT 2. A.nidulans HindIII-EcoRI 3040bp, gpdA gene
FT -> pAN52-3 5670bp"
FT promoter 1..2129
FT /note="PRO A. nidulans gpdA gene;
FT EXPERIMENTAL [1],[2]"
FT misc_feature 2130..2130
FT /note="A. nidulans gpdA gene major
FT transcription start site; EXPERIMENTAL [1]"
FT intron 2178..2293
FT /note="EXPERIMENTAL [1]"
FT CDS 2302..2313
FT /note="gpdA secretion signal sequence very similar to
FT that of the A. niger glucoamylase gene;
FT EXPERIMENTAL [1] [2]"
FT terminator 2305..3036
FT /note="TER A. nidulans trpC gene terminator region
FT (containing CDS); EXPERIMENTAL [2],[3];
FT contains the 3'end of the trpC
FT transcript including 50 codons; two major
FT transcription ends have been identified"
FT misc_difference 2574..2584
FT /note="replace(2574..2584,gccttncaggc);
FT introduction of one base at this position generates
FT an BlgI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 2783..2791
FT /note="replace(2783..2791,cagcncctg);
FT introduction of one base at this position generates
FT an AlwNI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 2871..2881
FT /note="replace(2871..2881,gaaatcanttc);
FT introduction (actual sequence could be deletion as
FT well) of a base at this position results in the
FT elimination of an XmnI site which was shown to be
FT absent by restriction digestion [3]"
FT mutation 2908..2915
FT /note="replace(2908..2915,catgcatg);
FT A NcoI site present at this position was removed by
FT digestion, treatment with klenow polymerase and
FT religation [2]"
FT polyA_site 2564..2574
FT /note="partial"
FT polyA_site 2837..2847
FT /note="partial"
FT misc_feature 3037..5670
FT /note="from pUC18 HindIII-EcoRI [2]"
FT misc_feature 1..5670
FT /note="fungal expression vector pAN52-3;
FT EXPERIMENTAL [2]"
XX
SQ Sequence 5670 BP; 1423 A; 1429 C; 1365 G; 1450 T; 3 other;
gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
cccgcttgag cagacatcac catggaagct tgagatccac ttaacgttac tgaaatcatc
aaacagcttg acgaatctgg atataagatc gttggtgtcg atgtcagctc cggagttgag
acaaatggtg ttcaggatct cgataagata cgttcatttg tccaagcagc aaagagtgcc
ttctagtgat ttaatagctc catgtcaaca agaataaaac gcgttttcgg gtttacctct
tccagataca gctcatctgc aatgcattaa tgcattgact gcaacctagt aacgccttnc
aggctccggc gaagagaaga atagcttagc agagctattt tcattttcgg gagacgagat
caagcagatc aacggtcgtc aagagaccta cgagactgag gaatccgctc ttggctccac
gcgactatat atttgtctct aattgtactt tgacatgctc ctcttcttta ctctgatagc
ttgactatga aaattccgtc accagcncct gggttcgcaa agataattgc atgtttcttc
cttgaactct caagcctaca ggacacacat tcatcgtagg tataaacctc gaaatcantt
cctactaaga tggtatacaa tagtaaccat gcatggttgc ctagtgaatg ctccgtaaca
cccaatacgc cggccgaaac ttttttacaa ctctcctatg agtcgtttac ccagaatgca
caggtacact tgtttagagg taatccttct ttctagagct tggcactggc cgtcgtttta
caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
cacacaggaa acagctatga ccatgattac
//