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ID PAN524 preliminary; circular DNA; SYN; 5748 BP.
XX
AC Z32699;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pAN52-4 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 999-2572
RC M13mAN5-7B from M13mp11 & gpdA gene
RC from pAN5-41B
RC from pAN923-41B
RC from pAN923-42B
RA Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT "Functional elements in the promoter region of the Aspergillus
RT nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT dehydrogenase";
RL Gene 93:101-109(1990).
XX
RN [2]
RP 1-5748
RC pAN series from gpdA gene
RA Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA Van Den Hondel C.A.M.J.J.;
RT "Vectors for efficient production of homologous and heterologous
RT proteins in filamentous fungi";
RL Unpublished (1994).
XX
RN [3]
RP 1-5748
RC pAN52-4
RA Punt P.;
RT ;
RL Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL Rijkswik, The Netherlands.
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CC NCBI gi: 475163
CC NM (pAN52-4)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pAN52-4)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 EcoRI-HindIII 2635bp 451..2686..400
FT 2. A.nidulans HindIII-EcoRI 3110bp, gpdA gene
FT -> pAN52-4 5748bp"
FT promoter 1..2129
FT /note="PRO A. nidulans gpdA gene;
FT EXPERIMENTAL [1],[2]"
FT misc_feature 2130..2130
FT /note="A. nidulans gpdA gene major
FT transcription start site; EXPERIMENTAL [1]"
FT intron 2178..2293
FT /note="EXPERIMENTAL [1]"
FT sig_peptide 2302..2355
FT /note="gpdA secretion signal sequence very similar to
FT that of the A. niger glucoamylase gene #X00712;
FT EXPERIMENTAL [1] [2]"
FT CDS 2302..2391
FT /note="gpdA secretion signal sequence very similar to
FT that of the A. niger glucoamylase gene;
FT EXPERIMENTAL [2]"
FT misc_feature 2356..2375
FT /note="PRO_PEPTIDE sequence for putative propeptide,
FT contains dibasic processing site (LYS-ARG) [2]"
FT terminator 2392..3115
FT /note="TER A. nidulans trpC gene terminator region
FT (containing CDS); EXPERIMENTAL [2],[3];
FT contains the 3'end of the trpC
FT transcript including 50 codons; two major
FT transcription ends have been identified"
FT misc_difference 2652..2662
FT /note="replace(2652..2662,gccttncaggc);
FT introduction of one base at this position generates
FT an BlgI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 2861..2869
FT /note="replace(2861..2869,cagcncctg);
FT introduction of one base at this position generates
FT an AlwNI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 2949..2959
FT /note="replace(2949..2959,gaaatcanttc);
FT introduction (actual sequence could be deletion as
FT well) of a base at this position results in the
FT elimination of an XmnI site which was shown to be
FT absent by restriction digestion [3]"
FT mutation 2986..2993
FT /note="replace(2986..2993,catgcatg);
FT A NcoI site present at this position was removed by
FT digestion, treatment with klenow polymerase and
FT religation [2]"
FT polyA_site 2642..2652
FT /note="partial"
FT polyA_site 2915..2925
FT /note="partial"
FT misc_feature 3115..5748
FT /note="from pUC18 HindIII-EcoRI [2]"
FT misc_feature 1..5748
FT /note="fungal expression vector pAN52-2;
FT EXPERIMENTAL [2]"
XX
SQ Sequence 5748 BP; 1435 A; 1454 C; 1387 G; 1469 T; 3 other;
gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
cccgcttgag cagacatcac catgggcttc cgatctctac tcgccctgag cggcctcgtc
tgcaacgggt tggcaaatgt gatttccaag cgcgctggat ccgaagcttg agatccactt
aacgttactg aaatcatcaa acagcttgac gaatctggat ataagatcgt tggtgtcgat
gtcagctccg gagttgagac aaatggtgtt caggatctcg ataagatacg ttcatttgtc
caagcagcaa agagtgcctt ctagtgattt aatagctcca tgtcaacaag aataaaacgc
gttttcgggt ttacctcttc cagatacagc tcatctgcaa tgcattaatg cattgactgc
aacctagtaa cgccttncag gctccggcga agagaagaat agcttagcag agctattttc
attttcggga gacgagatca agcagatcaa cggtcgtcaa gagacctacg agactgagga
atccgctctt ggctccacgc gactatatat ttgtctctaa ttgtactttg acatgctcct
cttctttact ctgatagctt gactatgaaa attccgtcac cagcncctgg gttcgcaaag
ataattgcat gtttcttcct tgaactctca agcctacagg acacacattc atcgtaggta
taaacctcga aatcanttcc tactaagatg gtatacaata gtaaccatgc atggttgcct
agtgaatgct ccgtaacacc caatacgccg gccgaaactt ttttacaact ctcctatgag
tcgtttaccc agaatgcaca ggtacacttg tttagaggta atccttcttt ctagagcttg
gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
gtgagcggat aacaatttca cacaggaaac agctatgacc atgattac
//