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ID PAN562H preliminary; circular DNA; SYN; 6114 BP.
XX
AC Z32690;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pAN56-2H - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-850
RC pABpyrGNot from pBluescript & linker & pAB94-11, pyrG* gene
RC pAN52-7NotI from pAN52-1NotI & A.niger glaA gene promoter
RC pGUS54 from pABpyrGNot & pNOM102, E. coli uidA gene
RC pGUS64 from pAN52-7NotI & pNOM102, E. coli uidA gene
RC plasmid from pMTL25P & pAN52-6NotI, glaA gene promoter
RC pAN4-1 from pAN4
RC pAN4-cos1 from pAN4 & lambda, cos
RC pAB6-15 from plasmid & pAN4-1
RC pAB6-18 from plasmid & pAN4-cos1
RA Verdoes J.C., Punt P.J., Stouthamer A.H., Van Den Hondel C.A.M.J.J.;
RT "The effect of multiple copies of the upstream region of the
RT Aspergillus niger glucoamylase-encoding gene on expression";
RL Gene 145:179-187(1994).
XX
RN [2]
RP 1-6114
RC pAN56-2H, pAN series from gpdA gene
RA Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA Van Den Hondel C.A.M.J.J.;
RT "Vectors for efficient production of homologous and heterologous
RT proteins in filamentous fungi";
RL Unpublished (1994).
XX
RN [3]
RP 1-6114
RC pAN56-2H
RA Punt P.;
RT ;
RL Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL Rijkswik, The Netherlands.
XX
CC NCBI gi: 474930
CC NM (pAN56-2H)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pAN56-1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pTZ19R 2863bp
FT 2. Aspergillus niger, gLAA promoter
FT 3. Aspergillus niger, GLAA2 gene
FT 4. Aspergillus nidulans, trpC gene terminator
FT 5. lambda, cos
FT -> pAN56-1 6100bp
FT 1. pAN56-1
FT -> pAN56-2H 6114bp"
FT promoter 36..781
FT /note="PRO A. niger gLAA gene;
FT EXPERIMENTAL [1] [2]"
FT misc_feature 2134..2134
FT /note="A. niger gLAA gene major
FT transcription start site; EXPERIMENTAL [1]"
FT misc_RNA 851..2719
FT /note="A.niger glucoamylase gene (encoding GII form
FT only) GLAA2 gene; EXPERIMENTAL [2];
FT #XOO712 with several modifications; see other
FT features; this region contains 4 introns"
FT sig_peptide 815..904
FT /note="glaa secretion signal sequence
FT identical to that of the published glaa gene;
FT EXPERIMENTAL [2]"
FT misc_feature 905..922
FT /note="PRO_PEPTIDE sequence for putative propeptide,
FT contains dibasic processing site (LYS-ARG) [2]"
FT misc_feature 925..2617
FT /note="this region was taken from accession X00712.
FT GLAA gene present in PAN56-1 is from another A.niger
FT (N402) and could therefore have minor deviations from
FT the sequence given here [2]"
FT terminator 2751..3474
FT /note="TER A. nidulans trpC gene terminator region
FT (containing CDS); EXPERIMENTAL [2],[3];
FT contains the 3'end of the trpC
FT transcript including 50 codons; two major
FT transcription ends have been identified"
FT misc_difference 3011..3021
FT /note="replace(3011..3021,gccttncaggc);
FT introduction of one base at this position generates
FT an BlgI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 3220..3228
FT /note="replace(3220..3228,cagcncctg);
FT introduction of one base at this position generates
FT an AlwNI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 3308..3318
FT /note="replace(3308..3318,gaaatcanttc);
FT introduction (actual sequence could be deletion as
FT well) of a base at this position results in the
FT elimination of an XmnI site which was shown to be
FT absent by restriction digestion [3]"
FT mutation 3345..3352
FT /note="replace(3345..3352,catgcatg);
FT A NcoI site present at this position was removed by
FT digestion, treatment with klenow polymerase and
FT religation [2]"
FT polyA_site 3001..3011
FT /note="partial"
FT polyA_site 3274..3284
FT /note="partial"
FT misc_feature 3474..6114
FT /note="from pUC18 HindIII-EcoRI [2]"
FT misc_feature 1..6114
FT /note="fungal expression/secretion vector pAN56-1;
FT EXPERIMENTAL [2]"
XX
SQ Sequence 6114 BP; 1429 A; 1617 C; 1545 G; 1520 T; 3 other;
gcggccgctg tgcagaattc gagctcggta cccggggatc cgaactccaa ccggggggag
tacattgagt ggccgcagtg gaaggaatcg cggcagttga tgaatttcgg agcgaacgac
gccagtctcc ttacggatga tttccgcaac gggacatatg agttcatcct gcagaatacc
gcggcgttcc acatctgatg ccattggcgg aggggtccgg acggtcagga acttagcctt
atgagatgaa tgatggacgt gtctggcctc ggaaaaggat atatggggat cataatagta
ctagccatat taatgaaggg catataccac gcgttggacc tgcgttatag cttcccgtta
gttatagtac catcgttata ccagccaatc aagtcaccac gcacgaccgg ggacggcgaa
tccccgggaa ttgaaagaaa ttgcatccta ggccagtgag gccagcgatt ggccacctct
ccaagcacac agggccattc tgcagcgctg gtggattcat cgcaatttcc cccggcccgg
cccgacaccg ctataggctg gttctcccac accatcggag attcgtcgcc taatgtctcg
tccgttcaca agctgaagag cttgaagtgg cgagatgtct ctgcaggaat tcaagctaga
tgctaagcga tattgcatgg caatatgtgt tgatgcatgt gcttcttcct tcagcttccc
ctcgtgcaga tgaggtttgg ctataaattg aagtggttgg tcggggttcc gtgaggggct
gaagtgcttc ctccctttta gacgcaactg agagcctgag cttcatcccc agcatcatta
cacctcagca atgtcgttcc gatctcttct cgccctgagc ggccttgtct gcacagggtt
ggcaaatgtg atttccaagc gcgcgacctt ggattcatgg ttgagcaacg aagcgaccgt
ggctcgtact gccatcctga ataacatcgg ggcggacggt gcttgggtgt cgggcgcgga
ctctggcatt gtcgttgcta gtcccagcac ggataacccg gactgtatgt ttcgagctca
gatttagtat gagtgtgtca ttgattgatt gatgctgact ggcgtgtcgt ttgttgtaga
cttctacacc tggactcgcg actctggtct cgtcctcaag accctcgtcg atctcttccg
aaatggagat accagtctcc tctccaccat tgagaactac atctccgccc aggcaattgt
ccagggtatc agtaacccct ctggtgatct gtccagcggc gctggtctcg gtgaacccaa
gttcaatgtc gatgagactg cctacactgg ttcttgggga cggccgcagc gagatggtcc
ggctctgaga gcaactgcta tgatcggctt cgggcagtgg ctgcttgtat gttctccacc
cccttgcgtc tgatctgtga catatgtagc tgactggtca ggacaatggc tacaccagca
ccgcaacgga cattgtttgg cccctcgtta ggaacgacct gtcgtatgtg gctcaatact
ggaaccagac aggatatggt gtgtttgttt tattttaaat ttccaaagat gcgccagcag
agctaacccg cgatcgcaga tctctgggaa gaagtcaatg gctcgtcttt ctttacgatt
gctgtgcaac accgcgccct tgtcgaaggt agtgccttcg cgacggccgt cggctcgtcc
tgctcctggt gtgattctca ggcacccgaa attctctgct acctgcagtc cttctggacc
ggcagcttca ttctggccaa cttcgatagc agccgttccg gcaaggacgc aaacaccctc
ctgggaagca tccacacctt tgatcctgag gccgcatgcg acgactccac cttccagccc
tgctccccgc gcgcgctcgc caaccacaag gaggttgtag actctttccg ctcaatctat
accctcaacg atggtctcag tgacagcgag gctgttgcgg tgggtcggta ccctgaggac
acgtactaca acggcaaccc gtggttcctg tgcaccttgg ctgccgcaga gcagttgtac
gatgctctat accagtggga caagcagggg tcgttggagg tcacagatgt gtcgctggac
ttcttcaagg cactgtacag cgatgctgct actggcacct actcttcgtc cagttcgact
tatagtagca ttgtagatgc cgtgaagact ttcgccgatg gcttcgtctc tattgtggta
agtctacgct agacaagcgc tcatgttgac agagggtgcg tactaacaga agtaggaaac
tcacgccgca agcaacggct ccatgtccga gcaatacgac aagtctgatg gcgagcagct
ttccgctcgc gacctgacct ggtcttatgc tgctctgctg accgccaaca accgtcgtaa
ctccgtcgtg cctgcttctt ggggcgagac ctctgccagc agcgtgcccg gcacctgtgc
ggccacatct gccattggta cctacagcag tgtgactgtc acctcgtggc cgagtatcgt
ggctactggc ggcaccacta cgacggctac ccccactgga tccggcagcg tgacctcgac
cagcaagacc accgcgactg ctagcaagac cagcaccagt acgtcatcaa cctcctgtac
cactcccacg gcgccgtaaa gatctgctag aggatctggt tacagcttga gatccactta
acgttactga aatcatcaaa cagcttgacg aatctggata taagatcgtt ggtgtcgatg
tcagctccgg agttgagaca aatggtgttc aggatctcga taagatacgt tcatttgtcc
aagcagcaaa gagtgccttc tagtgattta atagctccat gtcaacaaga ataaaacgcg
ttttcgggtt tacctcttcc agatacagct catctgcaat gcattaatgc attgactgca
acctagtaac gccttncagg ctccggcgaa gagaagaata gcttagcaga gctattttca
ttttcgggag acgagatcaa gcagatcaac ggtcgtcaag agacctacga gactgaggaa
tccgctcttg gctccacgcg actatatatt tgtctctaat tgtactttga catgctcctc
ttctttactc tgatagcttg actatgaaaa ttccgtcacc agcncctggg ttcgcaaaga
taattgcatg tttcttcctt gaactctcaa gcctacagga cacacattca tcgtaggtat
aaacctcgaa atcanttcct actaagatgg tatacaatag taaccatgca tggttgccta
gtgaatgctc cgtaacaccc aatacgccgg ccgaaacttt tttacaactc tcctatgagt
cgtttaccca gaatgcacag gtacacttgt ttagaggtaa tccttctttc tagagcttgg
cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgcctgatgc ggtattttct
ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aatt
//