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ID PAST18A preliminary; circular DNA; SYN; 3052 BP.
XX
AC X53121;
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DT 15-AUG-1990 (Rel. 5, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE C. elegans phagemid vector pAST18a - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-3052
RC pAST18a
RA Fire A.Z.;
RT ;
RL Submitted (17-MAY-1990) on tape to the EMBL Data Library by:
RL Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL 115 West University Parkway, Baltimore MD 21210, USA.
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RN [2]
RP 1-3052
RC pAST18a from pAST & pUC18
RC pAST18b from pAST & pUC18
RC pAST19a from pAST & pUC19
RC pAST19b from pAST & pUC19
RC pICT19h from pAST & pIC19H
RC pICT19r from pAST & pIC19R
RC pPD26.14 from pAST19b & linker
RA Fire A.Z., Kondo K., Waterson R.;
RT "Vectors for low copy transformation of C. elegans";
RL Nucleic Acids Res. 18:4269-4270(1990).
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RN [3]
RC pHTC1 from Drosophila heat shock promoter & Tc1 ORF
RC pM2B from pShZ1 & C.elegans myoC gene
RC pAST or pAst from pUC12 & pRW91, C.elegans sup-7 gene
RA Fire A.Z.;
RT "Integrative transformation of Caenorhabditis elegans";
RL EMBO J. 5:2673-2680(1986).
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RN [4]
RC pTC1 from Tc1 element
RA Greenwald I.;
RT "lin-12, a nematode homeotic gene, is homologous to a set of
RT mammalian proteins that includes epidermal growth factor";
RL Cell 43:583-590(1985).
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RN [5]
RC pShZ1 from Drosophila heat shock promoter & pCH110
RA Munro S.;
RT ;
RL Unpublished (1986).
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RN [6]
RC pRW91, C.elegans sup-7 locus
RA Bolten S., Powell-Abel P., Fischoff D., Waterston R.;
RT "The sup-7(st5) X gene of Caenorhabditis elegans encodes a
RT tRNATrpUAG amber suppressor";
RL Proc. Natl. Acad. Sci. U.S.A. 81:6784-6788(1984).
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CC pAST18a is made from C. elegans sup-7 sequence (K02459) and
CC pUC18 sequence (M11662).
CC NM (pAST18a)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (C.elegans)
CC CP (low)
CC FN (cloning)
CC SE ()
CC PA (pUC18)(C.elegans sup-7)
CC BR (pAST18b)(pAST19a)(pAST19b)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. C.elegans, sup-7 locus
FT -> pRW91
FT 1. pRW91 EcoRI-SalI 1300bp, C.elegans sup-7
FT 2. pUC12 remove EcoRI-SalI 30bp 231..261, MCS/2650bp
FT -> pAst 4000bp
FT 1. pUC18 SspI 2686bp 2504..2504
FT 2. pAst SspI-SspI 366bp, sup-7
FT -> pAST18A 3052bp"
FT misc_feature 1..2501
FT /note="from pUC18"
FT misc_feature 2502..2869
FT /note="C. elegans sup-7 locus"
FT misc_feature 2870..3052
FT /note="from pUC18"
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SQ Sequence 3052 BP; 797 A; 735 C; 745 G; 775 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
gcgacacgga aatgttgaat actcatactc ttcctttttc aatgacaaga gagaataaca
tgactgcttg gcgcaatggt agcgcgttcg actctagatc gaaaggttgg gcgttcgatc
cgctcagtgg tcaaattttt tgttttttaa gtagtaatat aatacaattt aattcaaaat
tacacgcaaa aatttaataa agtagtccaa aatgctaatc gtgtgaaaaa atgccttctg
gtaatgtatt tctcacacga aaatttgttg aaattggttt ttgacacaag cgaatgttgc
aattattttc ttgatttttc tacaaatttg aaaatcaaat ttcacgactg caagtataac
attaaaaaca atatacccaa gcccacaaaa aacgtattct gaaaattgta ttattgaagc
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//