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ID PAT153 preliminary; circular DNA; SYN; 3658 BP.
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AC L08853; VB0007; IG0014;
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DT 11-DEC-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pAT153 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-7232
RC pAT153
RA Gilbert W.;
RT "VecBase 3.0";
RL Unpublished (1991).
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RN [2]
RC pAT201, pAT203 from ColE1 & pACYC184
RC pAT202, pAT204, pAT205, pAT273, pAT277 from ColE1 & pACYC184
RC pDS4101 from ColK & Tn1
RC pDS4151, pDS4152, pDS4155, pDS4156 from pDS4101
RC pAT153 from pBR322
RA Twigg A.J., Sherratt D.J.;
RT "Trans-complementable copy-number mutants of plasmid ColE1";
RL Nature 283:216-218(1980).
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CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Assembled by F. Pfeiffer, MPI, Martinsried
CC pAT153 was constructed by removal of HaeII-fragments B
CC and H (pos. 1649-2353) from pBR322.
CC There is confliciting information on the structure of pAT153.
CC some 200 bases may be erraneuosly missing from this sequence.
CC pAT153 has properties comparable to pBR327.
CC The bom or Mob site is lost.
CC pAT153 has somewhat higher copy number than pBR322.
CC pAT153 is derived from pBR322 by deletion of a 622 bp
CC HaeII fragment, bases 1730-2352.
CC NM (pAT153)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Amersham)
CC HO (E.coli)
CC CP (15 copies/cell)
CC FN (cloning)(greater gene dosage effect)(not mobilizable)
CC SE ()
CC PA (pBR322)
CC BR (pBR327)
CC OF (pWT511)(pWT551)(pWT571)
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 remove HaeII-HaeII 703bp 1649..2352,
FT \ B frag/nic 3658bp
FT -> pAT153 3658bp"
FT CDS 86..1276
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT rep_origin 1908..1908
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(2693..3381)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 3658 BP; 812 A; 1011 C; 952 G; 883 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//