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ID PBACPAK1 preliminary; circular DNA; SYN; 5461 BP.
XX
AC IG1033;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pBacPAK1 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pBacPAK1, pBacPAK8, pBacPAK9 from AcNPV
RA Kitts P.A., Possee R.D.;
RT "A method for producing recombinant baculovirus expression vectors
RT at high frequency";
RL Biotechniques 14:810-817(1993).
XX
CC NM (pBacPAK1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (CLONTECH)
CC HO (Spodoptera frugiperda IPLB-SF21)
CC CP ()
CC FN (transfer)(expression)
CC SE ()
CC PA (pAcYM1)(pAcCL29)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. AcNPV, polyhedrin gene
FT 2. pBR322, ori/amp gene
FT 3. M13, ori
FT -> pBacPAK1 5461bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT transposon 0..0
FT /note="TRN AcMNPV"
FT misc_binding 314..314
FT /note="SIT BstXI"
FT misc_binding 546..546
FT /note="SIT MluI"
FT misc_binding 1158..1158
FT /note="SIT EcoRV"
FT promoter 0..0
FT /note="PRO AcMNPV polyhedrin gene"
FT misc_binding 1253..1253
FT /note="SIT BamHI"
FT misc_binding 1324..1324
FT /note="SIT SnaBI"
FT polyA_signal 0..0
FT /note="PLA AcMNPV polyhedrin gene"
FT misc_binding 1687..1687
FT /note="SIT HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage M13"
FT misc_binding 2998..2998
FT /note="SIT DraIII"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3913..3913
FT /note="SIT ScaI"
FT misc_binding 4873..4873
FT /note="SIT AlwNI"
XX
SQ Sequence 5461 BP; 1432 A; 1184 C; 1258 G; 1587 T; 0 other;
aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccggg
ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
aaataaagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
aattatttct gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa
ttcagacaac acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc
tactaatggc ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg
ggctggcggc ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc
aaatgtctct ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgccgt
ttttggtttg accggtctga gacgagtgcg atttttttcg tttctaatag cttccaacaa
ttgttgtctg tcgtctaaag gtgcagcggg ttgaggttcc gtcggcattg gtggagcggg
cggcaattca gacatcgatg gtggtggtgg tggtggaggc gctggaatgt taggcacggg
agaaggtggt ggcggcggtg ccgccggtat aatttgttct ggtttagttt gttcgcgcac
gattgtgggc accggcgcag gcgccgctgg ctgcacaacg gaaggtcgtc tgcttcgagg
cagcgcttgg ggtggtggca attcaatatt ataattggaa tacaaatcgt aaaaatctgc
tataagcatt gtaatttcgc tatcgtttac cgtgccgata tttaacaacc gctcaatgta
agcaattgta ttgtaaagag attgtctcaa gctcggatcg atcccgcacg ccgataacaa
gccttttcat ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgcctgatg
cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
g
//