Return to this vector's summary.
ID PBACPAK8 preliminary; circular DNA; SYN; 5538 BP.
XX
AC IG1433;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pBacPAK8 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pBacPAK1, pBacPAK8, pBacPAK9 from AcNPV
RA Kitts P.A., Possee R.D.;
RT "A method for producing recombinant baculovirus expression vectors
RT at high frequency";
RL Biotechniques 14:810-817(1993).
XX
CC NM (pBacPAK8)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (CLONTECH)
CC HO (Spodoptera frugiperda IPLB-SF21)
CC CP ()
CC FN (transfer)(expression)
CC SE ()
CC PA (pAcYM1)(pAcCL29)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. AcNPV, polyhedrin gene
FT 2. pBR322, ori/amp gene
FT 3. M13, ori
FT -> pBacPAK8 5538bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT transposon 0..0
FT /note="TRN AcMNPV"
FT misc_binding 314..314
FT /note="SIT BstXI"
FT misc_binding 546..546
FT /note="SIT MluI"
FT misc_binding 1158..1158
FT /note="SIT EcoRV"
FT promoter 0..0
FT /note="PRO AcMNPV polyhedrin gene"
FT misc_binding 1253..1253
FT /note="SIT BamHI"
FT misc_binding 1324..1324
FT /note="SIT SnaBI"
FT polyA_signal 0..0
FT /note="PLA AcMNPV polyhedrin gene"
FT misc_binding 1687..1687
FT /note="SIT HindIII"
FT rep_origin 0..0
FT /note="ORI bacteriophage M13"
FT misc_binding 2998..2998
FT /note="SIT DraIII"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3913..3913
FT /note="SIT ScaI"
FT misc_binding 4873..4873
FT /note="SIT AlwNI"
XX
SQ Sequence 5538 BP; 1449 A; 1205 C; 1278 G; 1606 T; 0 other;
aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccctg
caggcctcga gttcgaatct agaagatctg gtaccgagct cgaattcccg ggcggccgct
taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc
ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc
gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
gccgattcat taatgcag
//