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ID PBD12 preliminary; circular DNA; SYN; 6667 BP.
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AC ATCC37101;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Bacillus/Staphylococcus plasmid vector pBD12 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pBD6 from pSA0501 & pUB110
RC pBD7 from B.licheniformis DNA & pUB110
RC pBD8 from pUB110 & pSA2100
RC pBD9 from pUB110 & pE194
RC pBD10, pBD11 from pBD8 & pE194
RC pBD12 from pUB110 & pCM194
RA Gryczan T.J., Dubnau D.;
RT "Construction and properties of chimeric plasmids in Bacillus
RT subtilis";
RL Proc. Natl. Acad. Sci. U.S.A. 75:1428-1432(1978).
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CC Created by Moore, July 1995, under contract with NCBI.
CC A general purpose plasmid vector for cloning in Bacillus subtilis. [2]
CC Medium is 1179 V Y medium.
CC NM (pBD12)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (Bacillus subtilis BD170)(Bacillus)(Staphylococcus aureus)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUB110)(pC194)
CC BR (pBD6)(pBD8)(pBD9)(pBD10)(pBD11)(pBD20)(pBD35)
CC OF (pBD64)
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pC194 2910bp
FT -> pCM194 2910bp
FT 1. pUB110 HpaII-HpaII 3757bp 2753..4548..1962,
FT \ kan gene/HpaII B fragment
FT 2. pCM194 HpaII 2910bp 974..974, cat gene
FT -> pBD12 6667bp"
FT - 1..973
FT /note="pC194 1..973 973bp
FT HpaII = C^CGG"
FT - 974..4730
FT /note="pUB110 2753..4548..1961 3757bp
FT HpaII = C^CGG"
FT - 4731..6667
FT /note="pC194 974..2910 1937bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-XbaI-BamHI-TacI-HindIII-
FT BglII"
FT rep_origin 0..0
FT /note="ORI Bacillus pUB110"
FT CDS 0..0
FT /note="ANT Bacillus chloramphenicol acetyltransferase
FT gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT CDS 0..0
FT /note="ANT Bacillus kanamycin resistance gene (kan)"
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SQ Sequence 6667 BP; 2037 A; 1301 C; 860 G; 2469 T; 0 other;
gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta gcctctttaa
ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat gcatcaaaga
ctttttgatt tatttattaa ataatcacta tctttaccag aatacttagc catttcatat
aattctttat tattattttg tcttattttt tgaacttgaa cttgtgttat ttctgaaatg
cccgttacat cacgccataa atctaaccat tcttgttggc taatataata tcttttatct
gtgaaatacg atttatttac tgcaattaac acatgaaaat gaggattata atcatctctt
tttttattat atgtaatctc taacttacga acatatccct ttataacact acctactttt
tttctcttta taagttttct aaaagaatta ttataacgtt ttatttcatt ttctaattca
tcactcatta cattaggtgt agtcaaagtt aaaaagataa actccttttt ctcttgctgc
ttaatatatt gcatcatcaa agataaaccc aatgcatctt ttctagcttt tctccaagca
cagacaggac aaaatcgatt tttacaagaa ttagctttat ataatttctg tttttctaaa
gttttatcag ctacaaaaga cagaaatgta ttgcaatctt caactaaatc catttgattc
tctccaatat gacgtttaat aaatttctga aatacttgat ttctttgttt tttctcagta
tacttttcca tgttataaca cataaaaaca acttagtttt cacaaactat gacaataaaa
aaagttgctt tttccccttt ctatgtatgt tttttactag tcatttaaaa cgatacatta
ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat aacttaaggg
taactagcct cgccggttgt ccattcatgg ctgaactctg cttcctctgt tgacatgaca
cacatcatct caatatccga atagggccca tcagtctgac gaccaagaga gccataaaca
ccaatagcct taacatcatc cccatattta tccaatattc gttccttaat ttcatgaaca
atcttcattc tttcttctct agtcattatt attggtccat tcactattct cattcccttt
tcagataatt ttagatttgc ttttctaaat aagaatattt ggagagcacc gttcttattc
agctattaat aactcgtctt cctaagcatc cttcaatcct tttaataaca attatagcat
ctaatcttca acaaactggc ccgtttgttg aactactctt taataaaata atttttccgt
tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc gtcatcatct
gtatgaatca aatcgccttc ttctgtgtca tcaaggttta attttttatg tatttctttt
aacaaaccac cataggagat taacctttta cggtgtaaac cttcctccaa atcagacaaa
cgtttcaaat tcttttcttc atcatcggtc ataaaatccg tatcctttac aggatatttt
gcagtttcgt caattgccga ttgtatatcc gatttatatt tatttttcgg tcgaatcatt
tgaactttta catttggatc atagtctaat ttcattgcct ttttccaaaa ttgaatccat
tgtttttgat tcacgtagtt ttctgtattc ttaaaataag ttggttccac acataccaat
acatgcatgt gctgattata agaattatct ttattattta ttgtcacttc cgttgcacgc
ataaaaccaa caagattttt attaattttt ttatattgca tcattcggcg aaatccttga
gccatatctg acaaactctt atttaattct tcgccatcat aaacattttt aactgttaat
gtgagaaaca accaacgaac tgttggcttt tgtttaataa cttcagcaac aaccttttgt
gactgaatgc catgtttcat tgctctcctc cagttgcaca ttggacaaag cctggattta
caaaaccaca ctcgatacaa ctttctttcg cctgtttcac gattttgttt atactctaat
atttcagcac aatcttttac tctttcagcc tttttaaatt caagaatatg cagaagttca
aagtaatcaa cattagcgat tttcttttct ctccatggtc tcacttttcc actttttgtc
ttgtccacta aaacccttga tttttcatct gaataaatgc tactattagg acacataata
ttaaaagaaa cccccatcta tttagttatt tgtttagtca cttataactt taacagatgg
ggtttttctg tgcaaccaat tttaagggtt ttcaatactt taaaacacat acataccaac
acttcaacgc acctttcagc aactaaaata aaaatgacgt tatttctata tgtatcaaga
taagaaagaa caagttcaaa accatcaaaa aaagacacct tttcaggtgc tttttttatt
ttataaactc attccctgat ctcgacttcg ttcttttttt acctctcggt tatgagttag
ttcaaattcg ttctttttag gttctaaatc gtgtttttct tggaattgtg ctgttttatc
ctttaccttg tctacaaacc ccttaaaaac gtttttaaag gcttttaagc cgtctgtacg
ttccttaagg aattccttag tgctttcata gattaaactc acatcacgct ttaaatcgct
tattttagac tttaaagact tgttttcttc aagcaactca ttataatcat ttacattttc
attaaatcgc tctacaagac cactatattt ttctttaact tgcccatgtt ctttacttaa
ttttttatat tctctcgcca tatcagtact catgagattt ctaacatgct gttttaacct
atcgttatct ctcgcagcag tcactaagtt tttataatca cgctccgata taacaacatt
tttggttggt ttcttttctg ttttcattat ttcttttccc aaaccaaaca tggacttttc
acccgttggc acttcaacac ttttcatgtg tcgtttcgct ggtacttcta aatctgattt
aactttatcg ctataagcag tccattcatc ttttttaact gctaaatttt tttctagaaa
atcaatctct ttttccaaag tttgtttttt aaatttagct gtctcaatat gtttacggtc
agagccacgt tcaccacgct tcaactcaaa accctgtttt ttcatatgct cggggaattt
atcttgtagc cataacagtt cttgacgatt aaacacattt tttccttgca gttttccatc
acgcataggc acaacaccta aatgcatgtg aggggtttgc tcatcattat gaactgttgc
ataagcaata ttttgcttgc catatcgttc ggaaaataat ttataacttt cctcaaaaaa
tcgtttttgt tctcctggat ccagttgctc aaaaaaatct cggtcagatg ttactagcaa
ctcatttaca agaacagcat ctttcctccg tttttcttgt acctgttttt tgtgattcaa
taatttcttt gacacgttcg ttgtaatcaa tatttttatc atttttcaaa tcataatttt
cacgtgttcg ctcatggtca atatcatcat tcgttctact ttttcgctct ctttgattat
gaaattgcat gccttttagt ccagctgatt tcactttttg cattctacaa actgcataac
tcatatgtaa atcgctcctt tttaggtggc acaaatgtga ggcattttcg ctctttccgg
caaccacttc caagtaaagt ataacacact atactttata ttcataaagt gtgtgctctg
cgaggctgtc ggcagtgccg accaaaacca taaaaccttt aagacctttc ttttttttac
gagaaaaaag aaacaaaaaa acctgccctc tgccacctca gcaaaggggg gttttgctct
cgtgctcgtt taaaaatcag caagggacag gtagtatttt ttgagaagat cactcaaaaa
atctccacct ttaaaccctt gccaattttt attttgtccg ttttgtctag cttaccgaaa
gccagactca gcaagaataa aatttttatt gtctttcggt tttctagtgt aacggacaaa
accactcaaa ataaaaaaga tacaagagag gtctctcgta tcttttattc agcaatcgcg
cccgattgct gaacagatta ataatagatt ttagcttttt atttgttgaa aaaagctaat
caaattgttg tcgggatcaa ttactgcaaa gtctcgttca tcccaccact gatcttttaa
tgatgtattg gggtgcaaaa tgcccaaagg cttaatatgt tgatataatt catcaattcc
ctctacttca atgcggcaac tagcagtacc agcaataaac gactccgcac ctgtacaaac
cggtgaatca ttactacgag agcgccagct tcatcacttg cctcccatag atgaatccga
acctcattac acattagaac tgcgaatcca tcttcatggt gaaccaaagt gaaacctagt
ttatcgcaat aaaaacctat actcttttta atatccccga ctggcaatgc cggcaatagt
tacccttatt atcaagataa gaaagaaaag gatttttcgc tacgctcaaa tcctttaaaa
aaacacaaaa gaccacattt tttaatgtgg tctttattct tcaactaaag cacccattag
ttcaacaaac gaaaattgga taaagtggga tatttttaaa atatatattt atgttacagt
aatattgact tttaaaaaag gattgattct aatgaagaaa gcagacaagt aagcctccta
aattcacttt agataaaaat ttaggaggca tatcaaatga actttaataa aattgattta
gacaattgga agagaaaaga gatatttaat cattatttga accaacaaac gacttttagt
ataaccacag aaattgatat tagtgtttta taccgaaaca taaaacaaga aggatataaa
ttttaccctg catttatttt cttagtgaca agggtgataa actcaaatac agcttttaga
actggttaca atagcgacgg agagttaggt tattgggata agttagagcc actttataca
atttttgatg gtgtatctaa aacattctct ggtatttgga ctcctgtaaa gaatgacttc
aaagagtttt atgatttata cctttctgat gtagagaaat ataatggttc ggggaaattg
tttcccaaaa cacctatacc tgaaaatgct ttttctcttt ctattattcc atggacttca
tttactgggt ttaacttaaa tatcaataat aatagtaatt accttctacc cattattaca
gcaggaaaat tcattaataa aggtaattca atatatttac cgctatcttt acaggtacat
cattctgttt gtgatggtta tcatgcagga ttgtttatga actctattca ggaattgtca
gataggccta atgactggct tttataatat gagataatgc cgactgtact ttttacagtc
ggttttctaa tgtcactaac ctgccccgtt agttgaagaa ggtttttata ttacagctcc
agatccatat ccttcttttt ctgaaccgac ttctcctttt tcgcttcttt attccaattg
ctttattgac gttgagcctc ggaaccctta acaatcccaa aacttgtcga atggtcggct
taatagctca cgctatgccg acattcgtct gcaagtttag ttaagggttc ttctcaacgc
acaataaatt ttctcggcat aaatgcgtgg tctaattttt atttttaata accttgatag
caaaaaatgc cattccaata caaaaccaca tacctataat cgataaccac ataacagtca
taaaaccact cctttttaac aaactttatc acaagaaata tttaaatttt aaatgccttt
attttgaatt ttaaggggca ttttaaagat ttaggggtaa atcatatagt tttatgccta
aaaacctaca gaagctttta aaaagcaaat atgagccaaa taaatatatt ctaattctac
aaacaaaaat ttgagcaaat tcagtgtcga ttttttaaga cactgcccag ttacatgcaa
attaaaattt tcatgatttt ttatagttcc taacagggtt aaaatttgta taacgaaagt
ataatgttta tataacgtta gtataataaa gcattttaac attatacttt tgataatcgt
ttatcgtcgt catcacaata acttttaaaa tactcgtgca taattcacgc tgacctccca
ataactacat ggtgttatcg ggaggtcagc tgttagcact tatattttgt tattgttctt
cctcgatttc gtctatcatt ttgtgattaa tttctctttt ttcttgttct gttaagtcat
aaagttcact agctaaatac tctttttgtt tccaaatata aaaaatttga tagatatatt
acggttg
//