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ID PBD7 preliminary; circular DNA; SYN; 2913 BP.
XX
AC X05337; X02396; K02560; IG5133;
XX
DT 01-OCT-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pBD7 - complete, MCS.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pCB111 from coxsackievirus B3
RC pC1B1 from pBD7 & pCB111
RC pC11B9 from pC1B1 & oligo
RA Dasmahapatra B.;
RT "Cell-free expression vector: use of insect virus translational
RT initiation signal for in vitro gene expression";
RL Meth. Enzymol. 217:143-151(1993).
XX
RN [2]
RP 1-79
RC pBD7 from pGEM-2 & p1B9SP, BBV 5'UTR/ATG
RA Dasmahapatra B., Rozhon E.J., Schwartz J.;
RT "pBD7, a novel cell-free expression vector with efficient
RT translation initiation signal";
RL Nucleic Acids Res. 15:3933-3933(1987).
XX
RN [3]
RC pUC13::BBVRNA1 from pUC13 & BBV RNA 1
RC pUC13::BBVRNA2 from pUC13 & BBV RNA 2
RC p1B.SP series, p1B9SP, p1B13SP from pSP64 & pUC13::BBVRNA1
RC p2B.SP series, p2B10SP, p2B48SP from pSP64 & pUC13::BBVRNA2
RC p1B.PM series from pPM1 & pUC13::BBVRNA1
RC p2B.PM series, p2B10PM8 from pPM1 & pUC13::BBVRNA2
RA Dasmahapatra B., Dasgupta R., Saunders K., Selling B., Gallagher T.,
RA Kaesberg P.;
RT "Infectious RNA derived by transcription from cloned cDNA copies
RT of the genomic RNA of an insect virus";
RL Proc. Natl. Acad. Sci. U.S.A. 83:63-66(1986).
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CC Created by Moore, July 1995, under contract with NCBI.
CC NCBI gi: 58256
CC MCS plus UTR. oligonucleotide linker.
CC NM (pBD7)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pGEM-2)(BBV)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC13 SmaI 2680bp 268..268, MCS
FT 2. BBV
FT reverse transcriptase
FT PstI-XbaI, RNA 1
FT -> pUC13::BBVRNA1
FT 1. pSP64 XbaI 2999bp 29..29
FT 2. pUC13::BBVRNA1 PstI-XbaI, BBV RNA 1
FT -> p1B9SP
FT 1. pGEM-2 remove EcoRI-PstI 40bp 11..51, MCS/2829bp
FT 2. p1B9SP PstI-EcoRI 79bp
FT \ ctgcagttttcgaaacaaataaaacagaaaagcgaacctaaacaatgact
FT \ ctagaggatccccgggcgagctcgaattc, BBV 5'UTR/ATG
FT -> pBD7 2908bp"
FT - 1..10
FT /note="pGEM-2 1..10 10bp
FT EcoRI = G^AATTC"
FT - 11..94
FT /note="84bp
FT \ aattcgagctcgcccggggatcctctagagtcattgtttaggttcgcttt
FT \ tctgttttctgttttatttgtttcgaaaactgca
FT PstI = CTGCA^G"
FT - 95..2913
FT /note="pGEM-2 51..2869 2819bp"
FT CDS 0..0
FT /note="ANT E. coli amp gene"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO T7"
FT misc_binding 0..0
FT /note="MCS HindIII-PstI-BBV UTR-XbaI-BamHI-SmaI-
FT SacI-EcoRI"
FT misc_feature 45..47
FT /note="insect virus translation initiation codon"
FT promoter 0..0
FT /note="PRO Sp6"
XX
SQ Sequence 2913 BP; 717 A; 746 C; 734 G; 716 T; 0 other;
gaatacacgg aattcgagct cgcccgggga tcctctagag tcattgttta ggttcgcttt
tctgttttct gttttatttg tttcgaaaac tgcagcccaa gcttccggtc tccctatagt
gagtcgtatt aatttcgata agccagctgc attaatgaat cggccaacgc gcggggagag
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatc gacgctctcc
cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
gaccatttcc ggggtgcgga acggcgttac cagaaactca gaaggttcgt ccaaccaaac
cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
gtatcataca catacgattt aggtgacact ata
//