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ID PBI101 preliminary; DNA; SYN; 5350 BP.
XX
AC U12639; IG1034; X04996; X04995; V00087; J01541; M61152; M38354;
AC S54410; M14641;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Higher plants/E.coli plasmid vector pBI101 - incomplete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RC pBI121 from pBI101.1 & CMV 35S promoter
RC pBI131 from pBI101.1 & tobacco rbcS promoter
RA Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
RT "GUS fusions: beta-glucuronidase as a sensitive and versatile gene
RT fusion marker in higher plants";
RL EMBO J. 6:3901-3907(1987).
XX
RN [2]
RP 1-5350
RC pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RA Wei W., Lindsey K.;
RT "T-DNA sequence of the GUS gene fusion vector pBI101";
RL Unpublished (1994).
XX
RN [3]
RP 1-5350
RC pBI101.1
RA Wei W.;
RT ;
RL Submitted (22-JUL-1994) by:
RL Wei W., Botany, University of Leicester, University Road,
RL Leicester, LE1 7RH, UK.
XX
CC NM (pBI101)
CC CM (no)
CC NA (ds-DNA)
CC TP (linear)
CC ST ()
CC TY (plasmid)
CC SP (CLONTECH)
CC HO (E.coli)(plant)
CC CP ()
CC FN (expression)(reporter)
CC SE ()
CC PA (pBIN19)
CC BR (pBI101.2)(pBI101.3)
CC OF (pBI121)(pBI221)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBIN19 EcoRI 11777bp 6773..6773, MCS
FT 2. pRAJ260 EcoRI-EcoRI, GUS gene
FT -> pBI101 5350bp"
FT rep_origin 0..0
FT /note="ORI E. coli RK2"
FT promoter 0..0
FT /note="PRO Agrobacterium Ti plasmid nopaline synthase"
FT CDS 386..1180
FT /note="ANT E. coli neomycin phosphotransferase II gene
FT (NPTII); neomycin resistance gene (neo)
FT E. coli kanamycin resistance gene (kan)"
FT misc_binding 0..0
FT /note="SIT PstI"
FT misc_binding 0..0
FT /note="SIT SphI"
FT terminator 0..0
FT /note="TER Agrobacterium Ti plasmid nopaline synthase"
FT misc_binding 2498..2538
FT /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI"
FT misc_feature 0..0
FT /note="ATG"
FT CDS 2552..4360
FT /note="GEN E. coli beta-glucuronidase gene (GUS)"
FT misc_binding 0..0
FT /note="SIT SnoBI"
FT misc_binding 0..0
FT /note="SIT SstI"
FT 3'UTR 4431..4683
FT /note="TER 3'UTR of the nopaline synthase gene"
FT repeat_unit 5326..5350
FT /note="the left border repeat"
FT misc_binding 0..0
FT /note="SIT EcoRI"
XX
SQ Sequence 5350 BP; 1273 A; 1345 C; 1420 G; 1312 T; 0 other;
gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
ctctagagga tccccgggtg gtcagtccct tatgttacgt cctgtagaaa ccccaacccg
tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat
tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag
ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg tctggtatca
gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc
ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg
ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat
caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac
cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccggaat
ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt
gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg
tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac
tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta
tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc ttcgcgtcgg
catccggtca gtggcagtga agggccaaca gttcctgatt aaccacaaac cgttctactt
tactggcttt ggtcgtcatg aagatgcgga cttacgtggc aaaggattcg ataacgtgct
gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca
ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga
tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa
gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca
ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat
tgccaacgaa ccggataccc gtccgcaagt gcacgggaat atttcgccac tggcggaagc
aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
gaacttcggt gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc
accatcgtcg gctacagcct cgggaattgc taccgagctc gaatttcccc gatcgttcaa
acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
atcgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt
cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
gggctattct tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat
tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat
taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
tatcctgcca
//