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ID PBLCAT3 preliminary; circular DNA; SYN; 4344 BP.
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AC X64409; ATCC37528;
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DT 17-JUN-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pBLCAT3 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-4344
RC pBLCAT3 from pUC18 & cat gene & SV40 small t intron/polyA & linker
RC pBLCAT2 from pBLCAT3 & HSV tk gene promoter
RA Luckow B.H., Schutz G.;
RT "CAT constructions with multiple unique restriction sites for
RT functional analysis of eukaryotic promoters and regulatory elements";
RL Nucleic Acids Res. 15:5490-5490(1987).
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CC Promoterless CAT vector for transient transfection experiments with
CC eukaryotic cells. Allows the analysis of foreign promoters and
CC enhancers.
CC
CC Developed to simplify construction of hybrid CAT genes.
CC Restriction digests of the clone give the following sizes (kb):
CC BamHI--4.4; HindIII--4.4; BglII--4.4; ClaI--uncut. (ATCC staff)
CC The ClaI site appears to have been destroyed during cloning.
CC (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NM (pBLCAT3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(E.coli DH1)(E.coli DH5)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (E.coli)(SV40)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 BamHI 2686bp 430..430, ori/amp gene
FT 2. E. coli BamHI-BamHI 1400bp, cat gene/no promoter
FT 3. SV40 BamHI-MboI 237bp 2534..2771,
FT \ small t intron/polyA
FT -> plasmid 4300bp
FT 1. plasmid BamHI 4300bp, 3' to cat
FT BglII-XhoI linker 12bp agatctctcgag
FT BamHI 4300bp, 5' to SV40/pUC18 430..430
FT fill in, to make ClaI
FT -> pBLCAT3 4344bp"
FT misc_binding 0..0
FT /note="MCS unique HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT BglII-XhoI-SmaI-KpnI-SstI-(EcoRI)"
FT misc_feature 1237..1850
FT /note="SV40 small T intron splice site"
FT polyA_signal 1847..2087
FT /note="PLA SV40 early"
FT CDS 488..1147
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (Cmr),
FT promoterless"
FT CDS complement(3284..4144)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ Sequence 4344 BP; 1159 A; 993 C; 1023 G; 1169 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
actctagagg atccagatct ggatctcgag gagcttggcg agattttcag gagctaagga
agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata
agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt
ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat ccccgggtac
cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
ggcgtatcac gaggcccttt cgtc
//