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ID PBLCAT3F1 preliminary; circular DNA; SYN; 4865 BP.
XX
AC IG9828;
XX
DT 01-JUL-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Vertebrate/E.coli phagemid vector pBLCAT3.f1 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pBLCAT3.f1 from pBLCAT3 & pUC-f1
RC pUC.A.1.5 from pUC18
RC pJFCAT1 from pBLCAT3.f1 & pUC.A.1.5
RC pGEM2-human-beta-globin from pGEM-2 & human beta-globin gene
RC pbetaGloXH from pGEM-7 series & pGEM2-human-beta-globin
RC pbetaGlo4 from pbetaGloXH & mouse tk promoter
RC pbetaGlo4pA from pbetaGlo4 & pJFCAT1
RC pTAG-1 from pbetaGlo4pA & pBLCAT3
RA Fridovich-Keil J.L., Gudas J.M., Bryan I.B., Pardee A.B.;
RT "Improved expression vectors for eukaryotic promoter/enhancer
RT studies";
RL Biotechniques 11:572-579(1991).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pBLCAT3.f1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (E.coli)(E.coli HB101)(vertebrate cells)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBLCAT3)(pUC-f1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBLCAT3 remove SmaI-KpnI 6bp 2095..2101,
FT \ 4338bp
FT 2. pUC-f1 KpnI-BamHI 519bp 413..932, phage f1 ori
FT :Klenow
FT -> pBLCAT3.f1 4857bp"
FT - 1..2094
FT /note="pBLCAT3 1..2094 2094bp
FT SmaI = CCC^GGG
FT BamHI = G^GATCC"
FT - 2095..2621
FT /note="pUC-f1 409..935 527bp complement
FT KpnI = GGTAC^C"
FT - 2622..4865
FT /note="pBLCAT3 2101..4344 2244bp"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT polyA_signal 0..0
FT /note="PLA SV40 major late gene trimer"
FT misc_binding 0..0
FT /note="MCS BamHI-HindIII-SphI-PstI-SalI-BamHI-BglII-
FT XhoI"
FT misc_binding 0..0
FT /note="SIT unique SphI-PstI-SalI-BglII-XhoI"
FT CDS 0..0
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (cat); chloramphenicol resistance gene (cmr/cml),
FT from pBLCAT3"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene, from pBLCAT3"
FT misc_feature 0..0
FT /note="SV40 small T intron splice site, from pBLCAT3"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT misc_binding 0..0
FT /note="SIT unique KpnI"
FT misc_feature 0..0
FT /note="from pBLCAT3 KpnI-SmaI"
FT misc_binding 0..0
FT /note="SIT unique SstI"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp), from pBLCAT3"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT from pBLCAT3"
XX
SQ Sequence 4865 BP; 1269 A; 1128 C; 1135 G; 1333 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
actctagagg atccagatct ggatctcgag gagcttggcg agattttcag gagctaagga
agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata
agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt
ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat ccccgatccc
cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta tacaatcttc
ctgtttttgg ggcttttctg attatcaacc ggggtgggta ccgagctcga attcgtaatc
atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
tcgtc
//