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ID PBLCAT5 preliminary; circular DNA; SYN; 4404 BP.
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AC M80483; ATCC77412;
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DT 25-FEB-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Vertebrate/E.coli plasmid vector pBLCAT5 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-4404
RC pBLCAT5 from pBLCAT2 & SV40
RC pBLCAT6 from pBLCAT3 & SV40
RA Boshart M., Klueppel M., Schmidt A., Schuetz G., Luckow B.H.;
RT "CAT reporter constructs with low background activity";
RL Gene 110:129-130(1992).
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CC Reporter plasmid for analysis of enhancer sequences in vertebrate
CC cells, using the herpes simplex virus (HSV) thymidine kinase promoter
CC to drive expression of the cat reporter gene.
CC Constructed from pBLCAT2 (ATCC 37527) by deletion of a 467 bp fragment
CC immediately upstream of the 5' polylinker and replacement by two
CC fragments, each containing the polyadenylation signal of the SV40
CC large T gene.
CC The vector contains the following restriction sites (approximate kb
CC from nt 1): BamHI--0.03;
CC BglI--3.23; ClaI--1.84; EcoRI--0.06, 0.46, 1.86; HindIII--0.0;
CC NcoI--0.76; PstI--0.01, 0.16; PvuI--3.48; PvuII--0.35, 2.04;
CC SacI--1.86; XhoI--0.20.
CC The order of the major features in the plasmid is: two SV40
CC polyadenylation signals - HindIII/MCS/BamHI - HSV tk promoter - cat -
CC SV40 small T splice signal - SV40 polyadenylation signal -
CC ClaI/MCS/SstI - pMB1 ori - ampR. [1]
CC Deposited by: M.D.Boshart
CC Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC NM (pBLCAT5)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBLCAT2 remove AatII-HindIII 466bp
FT \ 4428..4496..398/4030bp
FT 2. SV40 BamHI-HpaI 135bp 2534..2669, complement
FT 3. SV40 BamHI-BclI 237bp 2534..2771, complement
FT -> pBLCAT5 4402bp"
FT misc_binding 0..0
FT /note="MCS HindIII-SphI-SalI-XbaI-BamHI"
FT misc_binding 0..0
FT /note="MCS ClaI-SmaI-KpnI-SstI"
FT promoter 31..200
FT /note="PRO HSV thymidine kinase gene"
FT CDS 242..901
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (Cmr)"
FT misc_feature 991..1604
FT /note="SPL SV40 small T splice site"
FT polyA_signal 1601..1841
FT /note="PLA SV40 polyadenylation signal"
FT CDS complement(3038..3898)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp), partial"
FT polyA_signal 4030..4164
FT /note="PLA SV40 polyadenylation signal"
FT polyA_signal 4161..4401
FT /note="PLA SV40 polyadenylation signal"
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SQ Sequence 4404 BP; 1196 A; 1005 C; 976 G; 1227 T; 0 other;
aagcttgcat gcctgcaggt cgactctaga ggatccggcc ccgcccagcg tcttgtcatt
ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
caacagcgtg ccgcagatct cgaggagctt ggcgagattt tcaggagcta aggaagctaa
aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca
tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat
aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccgg gtaccgagct
cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
tgccacctga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
aatgtatctt atcatgtctg gatcataatc agccatacca catttgtaga ggttttactt
gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
gtatcttatc atgtctggat ctcg
//