Return to this vector's summary.
ID PBLCAT6 preliminary; circular DNA; SYN; 4256 BP.
XX
AC M80484; ATCC77413;
XX
DT 25-FEB-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Vertebrate/E.coli plasmid vector pBLCAT6 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-4256
RC pBLCAT5 from pBLCAT2 & SV40
RC pBLCAT6 from pBLCAT3 & SV40
RC [pBLCAT8+ from pBLCAT series]
RA Boshart M., Klueppel M., Schmidt A., Schuetz G., Luckow B.H.;
RT "CAT reporter constructs with low background activity";
RL Gene 110:129-130(1992).
XX
CC Promoter-cloning vector for analysis of promoters in vertebrate
CC cells, using the CAT reporter gene.
CC Constructed from pBLCAT3 (ATCC37528) by deletion of a 467 bp fragment
CC immediately upstream from the 5' polylinker and replacement by two
CC fragments, each containing the
CC polyadenylation signal of the SV40 large T gene.
CC The vector contains the following restriction sites (approximate kb
CC from nt 1): BglI--3.07;
CC ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33;
CC PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05.
CC The order of the major features in the plasmid is:
CC two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat -
CC SV40 small T splice site - SV40 polyadenylation
CC signal - ClaI/MCS/SstI - pMB1 ori - ampR. [1]
CC Deposited by: M.D.Boshart
CC Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC NM (pBLCAT6)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBLCAT3 remove AatII-HindIII 464bp
FT \ 4280..4344..400, 3880bp
FT 2. SV40 BamHI-HpaI 135bp 2534..2669, complement
FT 3. SV40 BamHI-BclI 237bp 2534..2771, complement
FT -> pBLCAT6 4256bp"
FT misc_binding 0..0
FT /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-BglII-
FT XhoI"
FT misc_binding 0..0
FT /note="MCS ClaI-SmaI-KpnI-SstI"
FT CDS 90..749
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (Cmr)"
FT misc_feature 839..1452
FT /note="SPL SV40 small T splice site"
FT polyA_signal 1449..1689
FT /note="PLA SV40 polyadenylation signal"
FT CDS complement(2886..3746)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp), partial"
FT polyA_signal 3878..4012
FT /note="PLA SV40 polyadenylation signal"
FT polyA_signal 4009..4249
FT /note="PLA SV40 polyadenylation signal"
XX
SQ Sequence 4256 BP; 1168 A; 954 C; 930 G; 1204 T; 0 other;
aagcttgcat gcctgcaggt cgactctaga ggatccagat ctggatctcg aggagcttgg
cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgccgga
tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
gtctggatcg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg taacttgttt attgcagctt
ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcataa
tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctcg
//