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ID PBLUBAC2 preliminary; circular DNA; SYN; 10271 BP.
XX
AC IG1160;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pBlueBac2 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC p2Bac from baculovirus
RC p2Blue from p2Bac
RC pBlueBac from AcNPV
RC pBlueBac2 from AcNPV
RC pBlueBacIII from AcNPV
RC pBlueBacHisA from AcNPV
RC pBlueBacHisB from AcNPV
RC pBlueBacHisC from AcNPV
RC pVL1392, pVL1393 from pAc series
RA ;
RT ;
RL The Digest 5:2-2(1992).
XX
CC NM (pBlueBac2)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(insect)
CC CP ()
CC FN (expression)(transfer)
CC SE (color blue/white)
CC PA ()
CC BR ()
CC OF (pBlueBacHisA)(pBlueBacHisB)(pBlueBacHisC)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 4361bp, ori/amp gene
FT 2. AcMNPV, polyhedrin/ETL promoters
FT 3. E. coli, lacZ gene
FT -> pBlueBac2 10271bp"
FT promoter 0..0
FT /note="PRO AcMNPV polyhedrin gene"
FT misc_binding 0..0
FT /note="MCS BamHI-BglII-PstI-NcoI-HindIII"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT transposon 0..0
FT /note="TRN AcMNPV"
FT misc_binding 226..226
FT /note="SIT SphI"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT misc_binding 2342..2342
FT /note="SIT XbaI"
FT misc_binding 6773..6773
FT /note="SIT SacI"
FT promoter complement(0..0)
FT /note="PRO AcMNPV ETL gene"
XX
SQ Sequence 10271 BP; 2735 A; 2489 C; 2441 G; 2606 T; 0 other;
tcgacagatc tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac
ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
acgaggccct ttcctctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac
ataaccttat gtatcataca catacgattt aggtgacact atagaactcg agtcgacgac
agcgaagtgt ctcgccacta caatgctgta gtaaaaatga aaaggcttgt tatcggcgtg
cggagcttga gacaatctct ttacaataca attgcttaca ttgagcggtt gttaaatatc
ggcacggtaa acgatagcga aattacaatg cttatagcag atttttacga tttgtattcc
aattataata ttgaattgcc accaccccaa gcgctgcctc gaagcagacg accttccgtt
gtgcagccag cggcgcctgc gccggtgccc acaatcgtgc gcgaacaaac taaaccagaa
caaattatac cggcggcacc gccgccacca ccttctcccg tgcctaacat tccagcgcct
ccaccaccac caccaccatc gatgtctgaa ttgccgcccg ctccaccaat gccgacggaa
cctcaacccg ctgcaccttt agacgacaga caacaattgt tggaagctat tagaaacgaa
aaaaatcgca ctcgtctcag accggtcaaa ccaaaaacgg cgcccgaaac cagtacaata
gttgaggtgc cgactgtgtt gcctaaagag acatttgagc ctaaaccgcc gtctgcatca
ccgccaccac ctccgcctcc gcctccgccg ccagccccgc ctgcgcctcc accgatggta
gatttatcat cagctccacc accgccgcca ttagtagatt tgccgtctga aatgttacca
ccgcctgcac catcgctttc taacgtgttg tctgaattaa aatcgggcac agttagattg
aaacccgccc aaaaacgccc gcaatcagaa ataattccaa aaagctcaac tacaaatttg
atcgcggacg tgttagccga cacaattaat aggcgtcgtg tggctatggc aaaatcgtct
tcggaagcaa cttctaacga cgagggttgg gacgacgacg ataatcggcc taataaagct
aacacgcccg atgttaaata tgtccaagct ttatttaacg tgtttacgtc gagtcaattg
tacactaacg acagtgatga aagaaataca aaagcgcata atattttgaa cgacgtcgaa
cctttattac aaaacaaaac acaaacgaat atcgacaaag ctagattgct gctacaagat
ttggcaagtt ttgtggcgtt gagcgaaaat ccattagata gtccagccat cggttcggaa
aaacaaccct tgtttgaaac taatcgaaac ctattttaca aatctattga ggatttaata
tttaaattca gatataaaga cgctgaaaat catttgattt tcgctctaac ataccaccct
aaagattata aatttaatga attattaaaa tacgtacaac aattgtctgt aaatcaacaa
cgcacagaat ctagcgctta ataaatgtac taataacaat gtatcgtgtt ttaatacgcc
ggaccagtga acagaggtgc gtctggtgca aactccttta ctttgaacac cagggaaact
tcaaggagaa tttcctcctc ttcagcagag tcggtaccga tgtaaacgat gggcttgtag
aagttctccc agatgacacg atcgatgaac tgttcgaacg agttggtgta ctcagagtga
aggttcatta ttgggcagcc gccgcccttc ttagccaggc tgatgcggta ctcgttgttg
ctgcccaccc atgaaggctc gacgatccta atcacgtcat gaggtacata gtcggggtcg
caacgcagag cgtgttgggc caggaatttg taacaacggt tgggtctagt gggacgcatg
ttgacaacaa ggaaaacatc catcacttct tggtcgttaa caatggggaa gctgtcttcc
atgaagcggg tccaagtttc cctgtagaac tcttttcctt tccatccaac gacaagcttc
atcgtgtcgg gtttaacatt acggatttcc ttgaagagag tgagtttttg gttcttgccg
ggtcccagga aaggatccgc tagcacgccc gatggtggga cggtatgaat aatccggaat
atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt atttatttgc
gagatggtta tcattttaat tatctccatg atatcaagcc acactggaca cgaaaaagtc
gaaggcactc gcttcgatca atggacaaag aatcaatcgt tcgtaggcaa cagaatgtcg
gaaagtttgc attggatgcg cggcgggtcc aacttgccgc aaaactgcgg cgagttcaac
gtggtgtcca gcctgttgat gtgcaacaat acgataatga aaaattgata acgcttgcac
gattgcaaac atgcacgctc ggttgaataa aagctcgcat cgtcgtcgta aaattagttg
tatcaaagag cagctgcaat tagaatcact gctaaaaatg atagatcccg tcgttttaca
acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag
tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga
ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggcg agggggatct
cgactctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
catgtctgga tctgatatca gcaactatat attgatagac atttccagtt tgtgatatta
gtttgtgcgt ctcattacaa tggctgttat ttttaacaac aaacaactgc tcgcagacaa
tagtatagaa aagggaggtg aactgttttt gtttaacggt tcgtacaaca ttttggaaag
ttatgttaat ccggtgctgc taaaaaatgg tgtaattgaa ctagaagaag ctgcgtacta
tgccggcaac atattgtaca aaaccgacga tcccaaattc attgattata taaatttaat
aattaaagca acacactccg aagaactacc agaaaatagc actgttgtaa attacagaaa
aactatgcgc agcggtacta tacaccccat taaaaaagac atatatattt atgacaacaa
aaaatttact ctatacgata gatacatata tggatacgat aataactatg ttaattttta
tgaggagaaa aatgaaaaag agaaggaata cgaagaagaa gacgacaagg cgtctagttt
atgtgaaaat aaaattatat tgtcgcaaat taactgtgaa tcatttgaaa atgattttaa
atattacctc agcgattata actacgcgtt ttcaattata gataatacta caaatgttct
tgttgcgttt ggtttgtatc gttaataaaa aacaaattta gcatttataa ttgttttatt
attcaataat tacaaatagg attgagaccc ttgcagttgc cagcaaacgg acagagcttg
tcgacagttt tcattttttg gcagtttggt ggtttataca catcttggga tttgattaaa
agatgcatga cttcacccac acacttggag tcgtggttgt agaaatgcgt cagagccttg
attaaacatc ggttttcaca cttggcacac gaaaccacgt ttgcaatgta acagtttagt
ataaacctct tcctgtacat accgccgcgc ggtacattgt tttctttcaa atcgttcaat
atttttttta tacacggagg catattaatc ttgttgttca gcatgtcgac gacatttttc
tttccttcta cgcattcttt catacaaatg tttctcttgc tgcgtttttt aaaattggaa
tttttaattt cattaatagt gggcggagcc gttaacagca tgtacaatcg cagtccggaa
attttgacca aaaccccgcc attgtagaat accgtgtatg gagttagggt caatttatca
ataaaatcat cgggatcaat taaatatgta gctttcttgt ctaaacatga cgctctaatg
agcggactcc acacgttata cgatgcattc gccatacttc gcggcttctc gcaccagcgg
tcctgattcg actaacggtc gattggatgg cttaatagtt ttaacgtcac cgacatactc
ggtaattttg tagtcggcag ttcttttggg cgtgatcgtt gtcactacgg gggtcatgtc
atcgcttcgt aaaaacttat tacaatcaaa gctcgtgccg gaacgcgtgc acagatcgtg
cacgttgcgt cgcaagctat ttaattcgcg ctttatttga tctctatcat agtcgtgttg
agatgcgaca acgggcgcat tgttaattct tgacgtgcga tacataatat attattggat
aaagttgcat taaatgaaac taacttacaa gatggctagt ttgttaaaat acgcgctgcg
cttgactcgg gaatacaaag aaaacattat tccacacttt gatcacttga ctcgattgcg
cgatttaatc gacggcatga ttaaaagcga ggatgtacaa cgttttaatc gcactaatcg
caatgattta atttcggctt gcatgcaaat caacgttcgg acgtacatgc ccaacgccac
gatagatatg cgcaaacaac ccaactgtat atattttcga atttgccaat attgccactt
ggaggccgac gtgccttcgc ccgacgatca ttcggtgtac agatacttgt gcgtcgcgtg
cggcacgccg ctggtcatcg accacccgct cgacgtgttc ggccacacgg aggaaggcgt
caacgaactg c
//