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ID PBLUE2SKP preliminary; circular DNA; SYN; 2961 BP.
XX
AC X52328;
XX
DT 10-MAY-1990 (Rel. 5, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pBluescript II SK(+) - complete.
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KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-2961
RC pBluescript II SK(+)
RA Thomas E.A.;
RT ;
RL Submitted (20-FEB-1990) on tape to the EMBL Data Library by:
RL Thomas E.A., Stratagene Cloning Systems,
RL 11099 North Torrey Pines Rd., La Jolla, CA 92037, USA.
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RN [2]
RC pBluescript II from pBluescript
RC pBluescript II KS, pBluescript II SK, from pBluescript
RC pBluescript II KS+ from pBluescript KS+ & linker
RC pBluescript II KS- from pBluescript KS- & linker
RC pBluescript II SK+ from pBluescript SK+ & linker
RC pBluescript II SK- from pBluescript SK- & linker
RA Alting-Mees M.A., Short J.M.;
RT "pBluescript II: gene mapping vectors";
RL Nucleic Acids Res. 17:9494-9494(1989).
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CC The SK designation indicates the polylinker is oriented such
CC that beta-galactosidase (lacZ) transcription proceeds through the SacI
CC site first and the KpnI site last.
CC pBluescript SK(+) carries an F1 origin of replication,
CC oriented such that transcription proceeds in
CC the same direction as beta-galactosidase (lacZ) transcription.
CC NM (pBluescript II SK+)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (coding)
CC TY (phagemid)
CC SP (Stratagene)
CC HO (E.coli)
CC CP ()
CC FN (mapping gene)(expression)
CC SE (color blue/white)
CC PA (pUC19)(lambda ZAPII)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBluescript SK+ 2964bp, 5' to T3 promoter
FT BssHII/BsePI linker 6bp gcgcgc
FT -> plasmid 2964bp
FT 1. plasmid 2964bp, 3' to T7 promoter
FT BssHII/BsePI linker 6bp gcgcgc
FT -> pBluescript II SK+ 2961bp"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1 intergenic region"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT misc_binding 0..0
FT /note="MCS (21 unique restriction sites)"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
XX
SQ Sequence 2961 BP; 746 A; 739 C; 754 G; 722 T; 0 other;
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca c
//