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ID PBLUEKSM preliminary; circular DNA; SYN; 2958 BP.
XX
AC X52326;
XX
DT 10-MAY-1990 (Rel. 5, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pBluescript KS(-) - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-2958
RC pBluescript KS(-)
RA Thomas E.A.;
RT ;
RL Submitted (20-FEB-1990) on tape to the EMBL Data Library by:
RL Thomas E.A., Stratagene Cloning Systems,
RL 11099 North Torrey Pines Rd., La Jolla, CA 92037, USA.
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RN [2]
RC pJF3 from pUC19 & T7 oligo
RC pBS or BlueScribe from pJF3 & T3 oligo
RC pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC pBluescript KS(+) or pBLS from pBS+ & linker
RC pBluescript KS(-) from pBS- & linker
RC pBluescript, pBluescript KS, pBluescript SK from pBS
RC pBST-B from pBS & pEMBL8
RC pBSITO#12 from pBST-B & pEMBL8
RC pPreB from pBSITO#12 & pBluescript SK(-)
RC lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC lambda LongD from lambda LongC
RC lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT excision properties";
RL Nucleic Acids Res. 16:7583-7600(1988).
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CC The KS designation indicates the polylinker is oriented such
CC that beta-galactosidase (lacZ) transcription proceeds through the KpnI
CC site first and the SacI site last.
CC pBluescript KS(-) carries an F1 origin of replication,
CC oriented such that transcription proceeds in the opposite direction
CC as beta-galactosidase (lacZ) transcription.
CC NM (pBluescript KS-)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (non-coding)
CC TY (phagemid)
CC SP (Stratagene)
CC HO (E.coli)
CC CP ()
CC FN (mapping gene)(expression)
CC SE (color blue/white)
CC PA (pUC19)(lambda ZAP)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBS- remove EcoRI-HindIII 51bp 882..933,
FT \ MCS/3153bp
FT Klenow:Klenow
FT linker KpnI-SacI 275bp
FT \ ggaaacagctatgaccatgattacgccaagctcgaaatta
FT \ accctcactaaagggaacaaaagctggagctccaccgcggtggcggccgc
FT \ tctagaactagtggatcccccgggctgcaggaattcgatatcaagcttat
FT \ cgataccgtcgacctcgaggggggcccggtacccaattcgccctatagtg
FT \ agtcgtattacaattcactggccgtcgttttacaa
FT -> pBluescript KS- 2958bp"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1 intergenic region"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT misc_binding 0..0
FT /note="MCS (21 unique restriction sites)"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
XX
SQ Sequence 2958 BP; 708 A; 754 C; 731 G; 765 T; 0 other;
cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgc
//