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ID PBR3226 preliminary; circular DNA; SYN; 5527 BP.
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AC IG9819;
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DT 01-JUL-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli plasmid vector pBR322-6 or pBR322-8 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pFL1, pFL2, pFL3, pFL4 from pBR322 & yeast 2 micron
RA Chevalier M.R., Bloch J.C., Lacroute F.;
RT "Transcriptional and translational expression of a chimeric
RT bacterial-yeast plasmid in yeasts";
RL Gene 11:11-19(1980).
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CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pBR322-6 or pBR322-8)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)(Saccharomyces cerevisiae)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR (pBR322)(YEp24)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 HindIII 4361bp 30..30
FT 2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT -> pBR322-6 5527bp"
FT - 1..29
FT /note="pBR322 1..29 29bp
FT HindIII = A^AGCTT"
FT - 30..1195
FT /note="YEp24 2273..3438 1166bp
FT HindIII = A^AGCTT"
FT - 1196..5527
FT /note="pBR322 30..4361 4332bp"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="ANT yeast URA3 gene"
FT misc_binding 0..0
FT /note="SIT PstI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT PstI"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT misc_binding 0..0
FT /note="SIT HindIII"
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SQ Sequence 5527 BP; 1365 A; 1396 C; 1404 G; 1362 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gcttttcaat tcaattcatc attttttttt
tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag
aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg
aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaagctt
taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca
cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
tcaagaa
//