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ID PBSM13M preliminary; circular DNA; SYN; 3204 BP.
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AC L08782; VB0043; IG0029;
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DT 11-DEC-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pBSM13- or BlueScribe M13- - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-3204
RC pBSM13- or BlueScribe M13-
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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RN [2]
RC pJF3 from pUC19 & T7 oligo
RC pBS or BlueScribe from pJF3 & T3 oligo
RC pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC pBluescript KS(+) from pBS+ & linker
RC pBluescript KS(-) from pBS- & linker
RC pBST-B from pBS & pEMBL8
RC pBSITO#12 from pBST-B & pEMBL8
RC pPreB from pBSITO#12 & pBluescript SK(-)
RC lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC lambda LongD from lambda LongC
RC lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT excision properties";
RL Nucleic Acids Res. 16:7583-7600(1988).
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CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC The rescued strand will contain the non-coding strand
CC of the beta-galactosidase (lacZ) gene.
CC NM (pBSM13-)(BlueScribe M13-)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (non-coding)
CC TY (phagemid)
CC SP (Stratagene)
CC HO (E.coli JM101)(E.coli JM109)
CC CP ()
CC FN (cloning)(transcription)(sequencing dideoxy)
CC SE (color blue/white)
CC PA (pBS)(pUC19)(PromT7)(PromT3)(PF1)
CC BR (pBSM13+)
CC OF (BlueKSm)(BlueSKm)
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBS NarI 2746bp 237..237
FT 2. M13 ?RsaI-DraI 454bp 5490..5944, ori
FT -> pBSM13- 3204bp"
FT misc_feature complement(1..238)
FT /note="from pUC19"
FT rep_origin complement(239..694)
FT /note="ORI bacteriophage M13 intergenic region,
FT RsaI-DraI fragment (454 bp) in NarI site"
FT misc_feature complement(695..858)
FT /note="from pUC19"
FT promoter complement(860..879)
FT /note="PRO bacteriophage T7"
FT misc_binding 881..937
FT /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-
FT KpnI-SacI-EcoRI"
FT promoter complement(943..962)
FT /note="PRO bacteriophage T3"
FT misc_feature complement(966..1202)
FT /note="from pUC19"
FT misc_feature complement(987..987)
FT /note="ATG E. coli lacZ gene start codon"
FT promoter complement(1110..1203)
FT /note="PRO E. coli lac gene"
FT misc_feature complement(1203..3204)
FT /note="from pUC19"
FT rep_origin 1379..1379
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(2147..2935)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 3204 BP; 776 A; 808 C; 802 G; 818 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgac
gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
gcgaatttta acaaaatatt aacgtttaca atttcgccat tcgccattca ggctgcgcaa
ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg
gggatcctct agagtcgacc tgcaggcatg caagcttttg ttccctttag tgagggttaa
ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
ggcgtatcac gaggcccttt cgtc
//