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ID PBSSKM preliminary; circular DNA; SYN; 2964 BP.
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AC L08786; VB0079;
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DT 04-JUN-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector BlueScribe SK- - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-2964
RC BlueScribe SK-
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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RN [2]
RC pJF3 from pUC19 & T7 oligo
RC pBS or BlueScribe from pJF3 & T3 oligo
RC pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC pBluescript KS(+) from pBS+ & linker
RC pBluescript KS(-) from pBS- & linker
RC pBST-B from pBS & pEMBL8
RC pBSITO#12 from pBST-B & pEMBL8
RC pPreB from pBSITO#12 & pBluescript SK(-)
RC lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC lambda LongD from lambda LongC
RC lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT excision properties";
RL Nucleic Acids Res. 16:7583-7600(1988).
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CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Sequence correction according to Stratagene.
CC The stand shown corresponds to pUC19c.
CC As in the published sequence of pUC19c, The M13mp19 lacZ region
CC is on the complementary strand.
CC This vector contains the f1 origin so that the minus strand
CC can be obtained upon f1 superinfection.
CC NM (BlueScribe SK-)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE (color)
CC PA (BlueScribe M13-)(pUC19)(PromT7)(PromT3)(bGalKS)(PF1)
CC BR (BlueScribe KS-)(BlueScribe SK-)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBS- remove EcoRI-HindIII 51bp 882..933,
FT \ MCS/3153bp
FT Klenow:Klenow
FT linker SacI-KpnI 275bp
FT \ ggaaacagctatgaccatgattacgccaagctcgaaatta
FT \ accctcactaaagggaacaaaagctggagctccaccgcggtggcggccgc
FT \ tctagaactagtggatcccccgggctgcaggaattcgatatcaagcttat
FT \ cgataccgtcgacctcgaggggggcccggtacccaattcgccctatagtg
FT \ agtcgtattacaattcactggccgtcgttttacaa
FT -> Bluescribe SK- 2964bp"
FT misc_feature 3..458
FT /note="from bacteriophage f1"
FT misc_feature complement(460..624)
FT /note="from pUC19"
FT promoter 626..645
FT /note="PRO bacteriophage T7"
FT misc_binding complement(653..760)
FT /note="MCS
FT SacI-SacII-BstXI-XmaIII-NotI-XbaI-SpeI-BamHI-SmaI-
FT PstI-EcoRI-EcoRV-HindIII-ClaI-SalI-XhoI-ApaI-DraII-
FT KpnI; BlueScribe KS- polylinker"
FT promoter complement(772..791)
FT /note="PRO bacteriophage T3"
FT misc_feature complement(795..1031)
FT /note="from pUC19"
FT misc_feature complement(1032..2964)
FT /note="from pUC19"
FT misc_feature 643..643
FT /note="start of T7 RNA synthesis"
FT misc_feature complement(774..774)
FT /note="start of T3 RNA synthesis"
FT CDS complement(1976..2764)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 2964 BP; 707 A; 757 C; 734 G; 766 T; 0 other;
ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
taacgcgaat tttaacaaaa tattaacgtt tacaatttcg cgccattcgc cattcaggct
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctg
//