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ID PCDM8 preliminary; circular DNA; SYN; 4356 BP.
XX
AC IG0331; M59925; M16445; X06296;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pCDM8 - complete.
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KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC from pCDM8
RA Bernot A., Auffray C.;
RT "Primary structure and ontogeny of an avian CD3 transcript";
RL Proc. Natl. Acad. Sci. U.S.A. 88:2550-2554(1991).
XX
RN [2]
RC pcDNA-IIb from pcDNA1 & synapsin IIb
RC from pMAMneo
RA Han H.Q., Nichols R.A., Rubin M.R., Bahler M., Greengard P.;
RT "Induction of formation of presynaptic terminals in neuroblastoma
RT cells by synapsin IIb";
RL Nature 349:697-700(1991).
XX
RN [3]
RC pT22 from human H-ras
RC pIA from adenovirus E1A
RA Prendergast G.C., Ziff E.B.;
RT "Methylation-sensitive sequence-specific DNA binding by the c-Myc
RT basic region";
RL Science 251:186-189(1991).
XX
RN [4]
RC [pCDM8 from pBR322 & polyoma & CMV & SV40 & M13]
RC [pcDNAI or pcDNA1 from pCDM8]
RC from pCDM8
RA Seed B.;
RT "An LFA-3 cDNA encodes a phospholipid-linked membrane protein
RT homologous to its receptor CD2";
RL Nature 329:840-842(1987).
XX
RN [5]
RC from pcDNA1
RA Thomsen G., Woolf T., Whitman M., Sokol S., Vaughan J., Vale W.,
RA Melton D.A.;
RT "Activins are expressed early in Xenopus embryogenesis and can
RT induce axial mesoderm and anterior structures";
RL Cell 63:485-493(1990).
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CC NM (pCDM8)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (E.coli MC1061P3)
CC CP ()
CC FN (expression)
CC SE ()
CC PA ()
CC BR ()
CC OF (pcDNAI)(pcDNAIAmp)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. piVX, ori
FT 2. M13, ori
FT 3. supF gene
FT 4. CMV, promoter
FT 5. SV40, splice/polyA
FT 6. polyoma, ori
FT 7. SV40, ori
FT -> pCDM8 4356bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 587..587
FT /note="SIT NheI"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage M13"
FT misc_binding 1179..1179
FT /note="SIT SacII"
FT CDS complement(0..0)
FT /note="GEN E. coli supF gene"
FT promoter 0..0
FT /note="PRO human cytomegalovirus (CMV)
FT immediate early gene"
FT misc_binding 1790..1790
FT /note="SIT NdeI"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="MCS HindIII-XbaI-XhoI-BstXI...-
FT BstXI-XhoI-PstI-NotI-XbaI"
FT repeat_unit 0..0
FT /note="RPT stuffer (358 bp)"
FT rep_origin 0..0
FT /note="ORI SV40"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 3220..3220
FT /note="SIT HpaI"
FT misc_binding 3353..3353
FT /note="SIT BamHI"
FT rep_origin 0..0
FT /note="ORI polyoma virus"
FT misc_binding 3742..3742
FT /note="SIT BclI"
FT misc_binding 4024..4024
FT /note="SIT ApaI"
FT misc_feature 0..0
FT /note="SPL SV40 splicing sequences"
FT terminator 0..0
FT /note="TER SV40 early gene"
FT misc_binding 3955..3955
FT /note="SIT SfiI"
XX
SQ Sequence 4356 BP; 1149 A; 1056 C; 1066 G; 1085 T; 0 other;
ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
acggggggtt cgtgcacaga gcccagcttg gagcgaacga cctacaccga actgagatac
ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
tgctatcttt atgatcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
aagggatttt agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac
actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc
agtaaaagca ttacccgtgg tggggttccc gagcggccaa agggagcaga ctctaaatct
gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt caaaagtccg
aaagaatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta
agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
tatcgaaatt aatacgactc actataggga gacccaagct tctagagatc cctcgacctc
gagatccatt gtgctggcgc ggattcttta tcactgataa gttggtggac atattatgtt
tatcagtgat aaagtgtcaa gcatgacaaa gttgcagccg aatacagtga tccgtgccgg
ccctggactg ttgaacgagg tcggcgtaga cggtctgacg acacgcaaac tggcggaacg
gttgggggtg cagcagccgg cgctttactg gcacttcagg aacaagcggg cgctgctcga
cgcactggcc gaagccatgc tggcggagaa tcatacgctt cggtgccgag agccgacgac
gactggcgct catttctgat cgggaatccc gcagcttcag gcaggcgctg ctcgcctacc
gccagcacaa tggatctcga gggatcttcc atacctacca gttctgcgcc tgcaggtcgc
ggccgcgact ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
gtgttaaact actgattcta attgttgtgg tattttagat tccaacctat ggaacttatg
aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
gaaaggtaga agaccccaag gactttcctt cagaattggt aagttttttg agtcatgctg
tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata
atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttatcactg
cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcccgcca
tggtatcaac gccatatttc tatttacagt agggacctct tcgttgtgta ggtaccgctg
tattcctagg gaaatagtag aggcaccttg aactgtctgc atcagccata tagcccccgc
tgttcgactt acaaacacag gcacagtact gacaaaccca tacacctcct ctgaaatacc
catagttgct agggctgtct ccgaactcat tacaccctac caagtgagag ctgtaatttc
gcgatcaagg gcagcgaggg cttctccaga taaaatagct tctgccgaga gtcccgtaag
ggtagacact tcagctaatc cctcgatgag gtctactaga atagtcagtg cggctcccat
tttgaaaatt cacttacttg atcagcttca gaagatgggc gagggcctcc aacacagtaa
ttttcctccc gactcttaaa atagaaaatg tcaagtcagt taaggaggaa gtggactaac
tgacgcagct ggccgtgcga catcctcttt taattagttg ctaggcaacg ccctccagag
ggcgtgtggt tttgcaagag gaagcaaaag cctctccacc caggcctaga atgtttccac
ccaatcatta ctatgacaac agctgttttt tttagtatta agcagaggcc ggggacccct
gggcccgctt actctggaga aaaagaagag aggcattgta gaggcttcca gaggcaactt
gtcaaaacag gactgcttct atttctgtca cactgtctgg ccctgtcaca aggtccagca
cctccatacc ccctttaata agcagtttgg gaacgggtgc gggtcttact ccgcccatcc
cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
tttttggagg cctaggcttt tgcaaaaagc taattc
//