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ID PCDNAIAMP preliminary; circular DNA; SYN; 4801 BP.
XX
AC IG1048; M59925; M16445; X06296;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pcDNAIAmp - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC [pCDM8 from pBR322 & polyoma & CMV & SV40 & M13]
RC [pcDNAI or pcDNA1 from pCDM8]
RC from pCDM8
RA Seed B.;
RT "An LFA-3 cDNA encodes a phospholipid-linked membrane protein
RT homologous to its receptor CD2";
RL Nature 329:840-842(1987).
XX
CC NM (pcDNAIAmp)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (E.coli)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pCDM8)
CC BR (pcDNAI)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pcDNAI 4033bp
FT 2. pBR322 800bp, amp gene
FT -> pcDNAIAmp 4801bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 587..587
FT /note="SIT NheI"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage M13"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1794..1794
FT /note="SIT PvuI"
FT promoter 0..0
FT /note="PRO human cytomegalovirus (CMV)
FT immediate early gene"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="MCS HindIII-BamHI-BstXI-EcoRI-EcoRV-
FT BstXI-NotI-XhoI-SphI-NsiI-XbaI"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT rep_origin 0..0
FT /note="ORI SV40"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 3816..3816
FT /note="SIT HpaI"
FT rep_origin 0..0
FT /note="ORI polyoma virus"
FT misc_feature 0..0
FT /note="SPL SV40 splicing sequences"
FT terminator 0..0
FT /note="TER SV40 early gene"
FT misc_binding 4880..4880
FT /note="SIT SfiI"
XX
SQ Sequence 4801 BP; 1300 A; 1126 C; 1133 G; 1242 T; 0 other;
ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
aagggatttt agacaggaac ggtacgccag ctggattacc aaagggcctc gtgatacgcc
tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
aacttcattt ttaatttctc tagcgcgttg acattgatta ttgactagtt attaatagta
atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccact agtaacggcc
gccagtgtgc tggaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct
agagggccct attctatagt gtcacctaaa tgctagagga tctttgtgaa ggaaccttac
ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat
agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
ttatcatgtc tggatcatcc cgccatggta tcaacgccat atttctattt acagtaggga
cctcttcgtt gtgtaggtac cgctgtattc ctagggaaat agtagaggca ccttgaactg
tctgcatcag ccatatagcc cccgctgttc gacttacaaa cacaggcaca gtactgacaa
acccatacac ctcctctgaa atacccatag ttgctagggc tgtctccgaa ctcattacac
cctccaaagt cagagctgta atttcgccat caagggcagc gagggcttct ccagataaaa
tagcttctgc cgagagtccc gtaagggtag acacttcagc taatccctcg atgaggtcta
ctagaatagt cagtgcggct cccattttga aaattcactt acttgatcag cttcagaaga
tggcggaggg cctccaacac agtaattttc ctcccgactc ttaaaataga aaatgtcaag
tcagttaagc aggaagtgga ctaactgacg cagctggccg tgcgacatcc tcttttaatt
agttgctagg caacgccctc cagagggcgt gtggttttgc aagaggaagc aaaagcctct
ccacccaggc ctagaatgtt tccacccaat cattactatg acaacagctg ttttttttag
tattaagcag aggccgggga cccctgggcc cgcttactct ggagaaaaag aagagaggca
ttgtagaggc ttccagaggc aacttgtcaa aacaggactg cttctatttc tgtcacactg
tctggccctg tcacaaggtc cagcacctcc ataccccctt taataagcag tttgggaacg
ggtgcgggtc ttactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaatt
c
//