Return to this vector's summary.
Return to this vector's summary.
LOCUS CVU47120 5474 bp DNA circular SYN 20-FEB-1996
DEFINITION Cloning vector pCI-neo, mammalian expression vector, complete
sequence.
ACCESSION U47120
NID g1197674
KEYWORDS .
SOURCE Cloning vector pCI-neo.
ORGANISM Cloning vector pCI-neo
artificial sequence; cloning vectors.
REFERENCE 1 (bases 1 to 5474)
AUTHORS Brondyk,W.H.
TITLE New Mammalian Expression Vector with a selectable marker: pCI-neo
JOURNAL Promega Notes 51, 10-14 (1995)
REFERENCE 2 (bases 1 to 5474)
AUTHORS Brondyk,W.H. and Groskreutz,D.J.
TITLE Direct Submission
JOURNAL Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation,
5445 East Cheryl Parkway, Madison, WI 53711, USA
COMMENT
NCBI gi: 1197674
FEATURES Location/Qualifiers
source 1..5474
/organism="Cloning vector pCI-neo"
enhancer 1..659
/note="Cytomegalovirus immediate-early enhancer"
promoter 669..750
/note="Cytomegalovirus immediate-early promoter"
intron 890..1022
/note="chimeric intron"
promoter 1067..1085
/note="T7 promoter"
misc_feature 1085..1137
/note="multiple cloning sites"
promoter 1137..1158
/note="T3 promoter"
polyA_signal 1167..1388
/note="SV40 late polyadenylation signal"
rep_origin 1483..1938
/note="phage f1 region"
promoter 2002..2420
/note="SV40 enhancer and early promoter - neomycin
selectable marker"
rep_origin 2318..2383
/note="SV40 minimum origin of replication - neomycin
selectable marker"
CDS 2465..3259
/gene="NeoR"
/note="NCBI gi: 1197675"
/codon_start=1
/function="neomycin resistance"
/product="neomycin phosphotransferase"
/db_xref="PID:g1197675"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3323..3371
/note="Synthetic polyadenylation signal - neomycin
selectable marker"
CDS 3782..4642
/gene="ampR"
/note="NCBI gi: 1197676"
/codon_start=1
/function="ampicillin resistance"
/product="beta-lactamase"
/db_xref="PID:g1197676"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1323 a 1362 c 1371 g 1418 t
ORIGIN
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
1081 ataggctagc ctcgagaatt cacgcgtggt acctctagag tcgacccggg cggccgcttc
1141 cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca
1201 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
1261 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
1321 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
1381 atgtggtaaa atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc
1441 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
1501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
1561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
1621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagagcttta cggcacctcg
1681 accgcaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
1741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
1801 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
1861 cggcctattg gttaaaaaat gagctgattt aacaaatatt taacgcgaat tttaacaaaa
1921 tattaacgtt tacaatttcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
1981 tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa
2041 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
2101 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
2161 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
2221 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
2281 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
2341 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
2401 taggcttttg caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagagc
2461 caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
2521 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
2581 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
2641 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
2701 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
2761 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
2821 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
2881 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
2941 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
3001 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
3061 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
3121 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
3181 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
3241 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
3301 aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
3361 ttttttgtgt gaatcgatag cgataaggat ccgcgtatgg tgcactctca gtacaatctg
3421 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
3481 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
3541 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
3601 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
3661 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
3721 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
3781 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
3841 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
3901 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
3961 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
4021 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
4081 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
4141 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
4201 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
4261 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
4321 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
4381 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
4441 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
4501 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
4561 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
4621 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
4681 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
4741 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
4801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
4861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
4921 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
4981 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
5041 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
5101 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
5161 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
5221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
5281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
5341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
5401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
5461 ggctcgacag atct
//