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ID PCR1000 preliminary; circular DNA; SYN; 2922 BP.
XX
AC IG1060;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pCR1000 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pKK421-1 from pKK233-2
RC pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC pSL180 from pUC18 & linker
RC pSL189 from pUC19 & linker
RC pSL190 from pSL189
RC pSL1180 from pUC118 & linker
RC pSL1190 from pUC119 & linker
RC pSL300 from pSLJ10 & linker
RC pSL250 from pSL300
RC pSL260 from pSL250
RC pSL270 from pSL250
RC pSL251 from pSL250
RC pSL261 from pSL260
RC pSL271 from pSL270
RC pSL301 from pSL300
RC pSE1200 from pUC120 & linker
RC pSE1201 from pUC120 & linker
RC pSE1202 from pUC120 & linker
RC pSE219 from pKK233-2
RC pSE220 from pSE219
RC pSE280 from pKK421-1
RC pSE380 from pTrc99A
RA Brosius J.;
RT "Superpolylinkers in cloning and expression vectors";
RL DNA 8:759-777(1989).
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CC NM (pCR1000)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (E.coli)
CC CP ()
CC FN (cloning cDNA)(deletions nested)
CC SE ()
CC PA (pUC19)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC19
FT -> pCR1000 2922bp"
FT CDS complement(0..0)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="MCS ApaI-XbaI-NsiI-SphI-XhoI-XmaIII-NotI-
FT BstXI-EcoRV-PstI-EcoRI-BstXI-XmaIII-SpeI-BamHI-
FT SacI-KpnI-HindIII-NsiI"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT misc_binding 511..511
FT /note="SIT AflIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1910..1910
FT /note="SIT SacII"
FT misc_binding 1999..1999
FT /note="SIT XmnI"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage f1"
XX
SQ Sequence 2922 BP; 677 A; 848 C; 765 G; 632 T; 0 other;
tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
cttttctact gaacgtgatc cccaccggaa ttgcggccgc ccaatacgca aaccgcctct
ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
aggaaacagc tatgaccatg attacgccaa gctcaatacg actcactata gggcccggta
ccgagctcac tagtttaatt aaaagcttat cggccgaggt gagaagggtt tcgatatcga
gagaaacggt tctcaccgcg gccgcgaatt cactggccgt cgttttacaa cgtcgtgact
gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
gcgaatggcg gccgcccggg gtgggcgaag aactccagca tgagatcccc gcgctggagg
atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
caatatcacg ggtatccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
agtcgttgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
catggatcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
gagcaaggcg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc
ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata
aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg
cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
gtttctgcgg actggctttc tacgtgttcc ggttccttta gcagcccttg cgccctgagt
gcttgcggca gcgtgaagct aattcatggt taaatttttg ttaaatcagc tcatttttta
accaataggc cgaaatcgca aaaatccctt aataaatcaa aagaatagcc cgagataggg
ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
aaagggcgaa aaaccgtcta tcagggcgat ggcggatcag ct
//