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ID PCRII preliminary; circular DNA; SYN; 3932 BP.
XX
AC IG1150;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pCRII - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pKK421-1 from pKK233-2
RC pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC pSL180 from pUC18 & linker
RC pSL189 from pUC19 & linker
RC pSL190 from pSL189
RC pSL1180 from pUC118 & linker
RC pSL1190 from pUC119 & linker
RC pSL300 from pSLJ10 & linker
RC pSL250 from pSL300
RC pSL260 from pSL250
RC pSL270 from pSL250
RC pSL251 from pSL250
RC pSL261 from pSL260
RC pSL271 from pSL270
RC pSL301 from pSL300
RC pSE1200 from pUC120 & linker
RC pSE1201 from pUC120 & linker
RC pSE1202 from pUC120 & linker
RC pSE219 from pKK233-2
RC pSE220 from pSE219
RC pSE280 from pKK421-1
RC pSE380 from pTrc99A
RA Brosius J.;
RT "Superpolylinkers in cloning and expression vectors";
RL DNA 8:759-777(1989).
XX
CC NM (pCRII)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (E.coli)
CC CP ()
CC FN (cloning cDNA)(deletions nested)
CC SE ()
CC PA (pUC19)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC19
FT -> pCRII 3932bp"
FT CDS complement(0..0)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="MCS ApaI-XbaI-NsiI-SphI-XhoI-XmaIII-NotI-
FT BstXI-EcoRV-PstI-EcoRI-BstXI-XmaIII-SpeI-BamHI-
FT SacI-KpnI-HindIII-NsiI"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT misc_binding 511..511
FT /note="SIT AflIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1910..1910
FT /note="SIT SacII"
FT misc_binding 1999..1999
FT /note="SIT XmnI"
FT rep_origin complement(0..0)
FT /note="ORI bacteriophage f1"
XX
SQ Sequence 3932 BP; 916 A; 1011 C; 1064 G; 941 T; 0 other;
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
ttgtgagcga ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc
gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
aatcgggggc tccctttagg gttccgattt agagctttac ggcacctcga ccgcaaaaaa
cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
aaccctatcg cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
ttaaaaaatg agctgattta acaaattcag ggcgcaaggg ctgctaaagg aaccggaaca
cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
atctcctgtc atctcgcctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
gcgaggatct cgtcgtgatc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
gccgcttttc tggattcaac gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
tagcgttgga tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
acgagttctt ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
taaagttctg ctatgtgata cactattatc ccgtattgac gccgggcaag agcaactcgg
tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
cataccaaac gacgagagtg acaccacgat gcctgtagca atgccaacaa cgttgcgcaa
actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actgaatgga
ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
tggtaagcgc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
cctgggcttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
gcgcagcgag tcagtgagcg aggaagcgga ag
//